Ligand (2S,5S)-5-carboxymethylproline

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Basic Ligand Information

Molecular Structure
Picture of (2S,5S)-5-carboxymethylproline (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C7H11NO4
(2S,5S)-5-carboxymethylproline
LIZWYFXJOOUDNV-WHFBIAKZSA-N
Synonyms:
(2S,5S)-carboxymethylproline, (2S,5S)-trans-Carboxymethylproline


Show all pahtways known for Show all BRENDA pathways known for (2S,5S)-5-carboxymethylproline

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
ATP + (2S,5S)-5-carboxymethylproline = AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-

In Vivo Product in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
ATP + (2S,5S)-5-carboxymethylproline = AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-

Product in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (11 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
pH 7.8, 22°C
0.28
-
pH 8.0, 25°, wild-type enzyme
0.36
-
pH 8.0, 25°C, mutant enzyme E380A
0.0067
-
pH 8.0, 25°C, mutant enzyme E380D
0.26
-
pH 8.0, 25°C, mutant enzyme E380Q
0.02
-
pH 8.0, 25°C, mutant enzyme Y345A
0.011
-
pH 8.0, 25°C, mutant enzyme Y345F
0.17
-
pH 8.0, 25°C, mutant enzyme Y345F/E380A
0.0051
-
pH 8.0, 25°C, mutant enzyme Y345F/E380D
0.0059
-
pH 8.0, 25°C, mutant enzyme Y345F/E380Q
0.011
-
pH 8.0, 25°C, wild-type enzyme
0.36
-

KM Value (10 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
pH 7.8, 22°C
0.23
-
pH 8.0, 25°C, mutant enzyme E380A
0.49
-
pH 8.0, 25°C, mutant enzyme E380D
0.36
-
pH 8.0, 25°C, mutant enzyme E380Q
0.075
-
pH 8.0, 25°C, mutant enzyme Y345A
1.7
-
pH 8.0, 25°C, mutant enzyme Y345F
0.27
-
pH 8.0, 25°C, mutant enzyme Y345F/E380A
0.4
-
pH 8.0, 25°C, mutant enzyme Y345F/E380D
0.49
-
pH 8.0, 25°C, mutant enzyme Y345F/E380Q
0.9
-
pH 8.0, 25°C, wild-type enzyme
0.35
-

References & Links

Links to other databases for (2S,5S)-5-carboxymethylproline

ChEBI
PubChem
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PubChem