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Ligand 3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Substrate (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.19

3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside + D-glucose = 3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltotetraoside + maltose

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2.4.1.19

3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside + maltose = 3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltotetraoside + maltotriose

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2.4.1.19

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (14 results)

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Show Turnover Number (14 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.4.1.19

0.122

-

mutant strain F283L, acceptor glucose, wild-type

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2.4.1.19

0.137

-

strain 1011, acceptor maltose, mutant F283L

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2.4.1.19

0.345

-

mutant strain F259L, acceptor glucose, wild-type

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2.4.1.19

0.423

-

strain 1011, acceptor maltose, mutant F259L

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2.4.1.19

1.97

-

mutant strain Y195L, acceptor glucose, wild-type

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2.4.1.19

2.2

-

strain 1011, acceptor maltose, mutant Y195L

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2.4.1.19

2.32

-

mutant strain Y195F, acceptor glucose, wild-type

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2.4.1.19

2.63

-

strain 1011, acceptor maltose, mutant F183L/F259L

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2.4.1.19

3.2

-

strain 1011, acceptor maltose, mutant Y195F

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2.4.1.19

3.38

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strain 1011, acceptor maltose, wild-type

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2.4.1.19

3.8

-

mutant strain F183L/F259L, acceptor glucose, wild-type

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2.4.1.19

4.07

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strain 1011, acceptor glucose, wild-type

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2.4.1.19

5.55

-

strain 1011, acceptor maltose, mutant F183L

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2.4.1.19

15.5

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mutant strain F183L, acceptor glucose, wild-type

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2.4.1.19

14 entries

K_M Value (12 results)

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Show KM Value (12 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.1.19

0.08

-

strain 1011, acceptor maltose, mutant F183L

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2.4.1.19

0.12

-

strain 1011, acceptor maltose, mutant F283L

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2.4.1.19

0.15

-

strain 1011, acceptor glucose, mutant F283L

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2.4.1.19

0.16

-

strain 1011, acceptor maltose, wild-type

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2.4.1.19

0.2

-

strain 1011, acceptor glucose, wild-type and mutant Y195F

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2.4.1.19

0.22

-

strain 1011, acceptor maltose, mutant Y195F

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2.4.1.19

0.23

-

strain 1011, acceptor glucose, mutant F183L

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2.4.1.19

0.24

-

strain 1011, acceptor maltose, mutant F183L/F259L

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2.4.1.19

0.31

-

strain 1011, acceptor maltose, mutant Y195L

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2.4.1.19

0.34

-

strain 1011, acceptor glucose, mutant Y195L and F183L/F259L

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2.4.1.19

0.48

-

strain 1011, acceptor maltose, mutant F259L

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2.4.1.19

0.56

-

strain 1011, acceptor glucose, mutant F259L

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2.4.1.19

12 entries

References & Links

Literature References (1)

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Show Reference (1 entry)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

488851

8061001

Four aromatic residues in the active center of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011: effects of replacements on substrate binding and cyclization characteristics

1994

Nakamura, A.; Haga, K.; Yamane, K.

Biochemistry

33

9929-9936

Links to other databases for 3-ketobutylidene-beta-2-chloro-4-nitrophenylmaltopentaoside

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Show Links (1 entry)

ChEBI

PubChem

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