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0.0168
-
pH 7.0, 25°C, recombinant enzyme
0.0168
-
wild-type, pH not specified in the publication, temperature not specified in the publication
0.05
-
mutant F159W, pH not specified in the publication, temperature not specified in the publication
0.07
-
mutant F200W, pH not specified in the publication, temperature not specified in the publication
9.2
-
presence of 50 mM phosphate, pH 7.0
12.5
-
presence of 50 mM phosphate, pH 7.0
13
-
presence of 100 mM phosphate, pH 7.0
13.9
-
presence of 100 mM phosphate, pH 7.0
16.3
-
presence of 100 mM phosphate, pH 7.0
39.3
-
presence of 50 mM phosphate, pH 7.0
0.0005
-
wild type enzyme, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.0021
-
wild-type, pH 7.0, 35°C
0.0024
-
mutant R252A, pH 7.0, 35°C
0.008
-
mutant D40A, pH 7.0, 35°C
0.037
-
wild-type, pH 7.5, 25°C
0.051
-
mutant C253A, ApH 7.5, 25°C
0.053
-
mutant C42A, pH 7.5, 25°C
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-
N-methyl nucleosidase from tea leaves
1988
Negishi, O.; Ozawa, T.; Imagawa, H.
Agric. Biol. Chem.
52
169-175
Formycins A and B and some analogues: selective inhibitors of bacterial (Escherichia coli) purine nucleoside phosphorylase
1992
Bzowska, A.; Kulikowska, E.; Shugar, D.
Biochim. Biophys. Acta
1120
239-247
Purine nucleoside phosphorylase. Structure-activity relationships for substrate and inhibitor properties of N-1-, N-7-, and C-8-substituted analogues; differentiation of mammalian and bacterial enzymes with N-1-methylinosine and guanosine
1988
Bzowska, A.; Kulikowska, E.; Darzynkiewicz, E.; Shugar, D.
J. Biol. Chem.
263
9212-9217
Properties of two unusual, and fluorescent, substrates of purine-nucleoside phosphorylase: 7-methylguanosine and 7-methylinosine
1986
Kulikowska, E.; Bzowska, A.; Wierzchowski, J.; Shugar, D.
Biochim. Biophys. Acta
874
355-363
Purine nucleoside phosphorylases: properties, functions, and clinical aspects
2000
Bzowska, A.; Kulikowska, E.; Shugar, D.
Pharmacol. Ther.
88
349-425
Nucleoside phosphotransferase from malt sprouts. I. Isolation, characterization and specificity of the enzyme
1986
Billich, A.; Stockhowe, U.; Witzel, H.
Biol. Chem. Hoppe-Seyler
367
267-278
Interactions of potent multisubstrate analogue inhibitors with purine nucleoside phosphorylase from calf spleen--kinetic and spectrofluorimetric studies
2003
Iwanow, M.; Magnowska, L.; Yokomatsu, T.; Shibuya, S.; Bzowska, A.
Nucleosides Nucleotides Nucleic Acids
22
1567-1570
Transition-state complex of the purine-specific nucleoside hydrolase of T. vivax: enzyme conformational changes and implications for catalysis
2006
Versees, W.; Barlow, J.; Steyaert, J.
J. Mol. Biol.
359
331-346
Probing the mechanism of purine nucleoside phosphorylase by steady-state kinetic studies and ligand binding characterization determined by fluorimetric titrations
2006
Wielgus-Kutrowska, B.; Bzowska, A.
Biochim. Biophys. Acta
1764
887-902
Purine nucleoside phosphorylase: a potential target for the development of drugs to treat T-cell- and apicomplexan parasite-mediated diseases
2007
Silva, R.G.; Nunes, J.E.; Canduri, F.; Borges, J.C.; Gava, L.M.; Moreno, F.B.; Basso, L.A.; Santos, D.S.
Curr. Drug Targets
8
413-422
Kinetic properties of Cellulomonas sp. purine nucleoside phosphorylase with typical and non-typical substrates: implications for the reaction mechanism
2005
Wielgus-Kutrowska, B.; Bzowska, A.
Nucleosides Nucleotides Nucleic Acids
24
471-476
Calcium-stimulated guanosine--inosine nucleosidase from yellow lupin (Lupinus luteus)
2006
Szuwart, M.; Starzynska, E.; Pietrowska-Borek, M.; Guranowski, A.
Phytochemistry
67
1476-1485
Interactions of calf spleen purine nucleoside phosphorylase with formycin B and its aglycone - spectroscopic and kinetic studies
2007
Wierzchowski, J.; Iwanska, B.; Bzowska, A.; Shugar, D.
Nucleosides Nucleotides Nucleic Acids
26
849-854
Overexpression, purification and characterization of functional calf purine nucleoside phosphorylase (PNP)
2008
Breer, K.; Girstun, A.; Wielgus-Kutrowska, B.; Staro?, K.; Bzowska, A.
Protein Expr. Purif.
61
122-130
A flexible loop as a functional element in the catalytic mechanism of nucleoside hydrolase from Trypanosoma vivax
2008
Vandemeulebroucke, A.; De Vos, S.; Van Holsbeke, E.; Steyaert, J.; Versees, W.
J. Biol. Chem.
283
22272-22282
Effect of the phosphate substrate on drug-inhibitor binding to human purine nucleoside phosphorylase
2008
Todorova, N.A.; Schwarz, F.P.
Arch. Biochem. Biophys.
480
122-131
Substrate specificity and kinetic mechanism of purine nucleoside phosphorylase from Mycobacterium tuberculosis
2009
Ducati, R.G.; Santos, D.S.; Basso, L.A.
Arch. Biochem. Biophys.
486
155-164
Structure and mechanism of the 6-oxopurine nucleosidase from Trypanosoma brucei brucei
2010
Vandemeulebroucke, A.; Minici, C.; Bruno, I.; Muzzolini, L.; Tornaghi, P.; Parkin, D.W.; Versees, W.; Steyaert, J.; Degano, M.
Biochemistry
49
8999-9010
Validation of the catalytic mechanism of Escherichia coli purine nucleoside phosphorylase by structural and kinetic studies
2011
Mikleusevic, G.; Stefanic, Z.; Narczyk, M.; Wielgus-Kutrowska, B.; Bzowska, A.; Luic, M.
Biochimie
93
1610-1622
Trimeric purine nucleoside phosphorylase: exploring postulated one-third-of-the-sites binding in the transition state
2012
Wielgus-Kutrowska, B.; Breer, K.; Hashimoto, M.; Hikishima, S.; Yokomatsu, T.; Narczyk, M.; Dyzma, A.; Girstun, A.; Staro?, K.; Bzowska, A.
Bioorg. Med. Chem.
20
6758-6769
Homooligomerization is needed for stability: a molecular modelling and solution study of Escherichia coli purine nucleoside phosphorylase
2014
Bertosa, B.; Mikleusevic, G.; Wielgus-Kutrowska, B.; Narczyk, M.; Hajnic, M.; Lescic Asler, I.; Tomic, S.; Luic, M.; Bzowska, A.
FEBS J.
281
1860-1871
Structural and biochemical characterization of the nucleoside hydrolase from C. elegans reveals the role of two active site cysteine residues in catalysis
2017
Singh, R.K.; Steyaert, J.; Versees, W.
Protein Sci.
26
985-996
Helicobacterpylori purine nucleoside phosphorylase shows new distribution patterns of open and closed active site conformations and unusual biochemical features
2018
Narczyk, M.; Bertosa, B.; Papa, L.; Vukovic, V.; Lescic Asler, I.; Wielgus-Kutrowska, B.; Bzowska, A.; Luic, M.; Stefanic, Z.
FEBS J.
285
1305-1325
Structural characterization of purine nucleoside phosphorylase from human pathogen Helicobacter pylori
2017
Stefanic, Z.; Mikleusevic, G.; Luic, M.; Bzowska, A.; Lescic Asler, I.
Int. J. Biol. Macromol.
101
518-526
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