Ligand nitroblue tetrazolium

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Basic Ligand Information

Molecular Structure
Picture of nitroblue tetrazolium (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C40H33ClN10O6
nitroblue tetrazolium
UZMZDVXLUXDHFH-UHFFFAOYSA-M
Synonyms:
2,2'-di-(p-nitrophenyl-5,5')-(diphenyl)-3,3'-(3,3'-dimethoxy-4,4'-diphenylene) ditetrazolium chloride, 5-nitroblue tetrazolium chloride, Nitro blue tetrazolium, nitrobluetetrazolium, nitroblue tetrazolium hydrochloride, nitroblue tetrazolium[side 2], p-nitrobluetetrazolium, reduced nitro blue tetrazolium, reduced nitroblue tetrazolium, reduced nitrobluetetrazolium, reduced p-nitrobluetetrazolium

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (34 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
methanol + nitroblue tetrazolium = formaldehyde + reduced nitroblue tetrazolium
show the reaction diagram
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polyvinyl alcohol + nitro blue tetrazolium = polyvinylketone + reduced nitro blue tetrazolium
show the reaction diagram
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D-fructose + nitro blue tetrazolium = 5-dehydro-D-fructose + ?
show the reaction diagram
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2-dehydro-D-gluconate + nitro blue tetrazolium = 2,5-didehydro-D-gluconate + ?
show the reaction diagram
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nitroblue tetrazolium + 2 NADPH = nitroblue tetrazolium formazan + 2 NADP+
show the reaction diagram
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formate + nitrobluetetrazolium = CO2 + reduced nitrobluetetrazolium
show the reaction diagram
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formate + nitroblue tetrazolium = CO2 + reduced nitroblue tetrazolium
show the reaction diagram
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NADH + nitroblue tetrazolium = NAD+ + reduced nitroblue tetrazolium
show the reaction diagram
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NADH + nitroblue tetrazolium = NAD+ + reduced nitroblue tetrazolium
show the reaction diagram
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succinate + nitro blue tetrazolium = fumarate + formazan
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glycine + 2,2'-di-(p-nitrophenyl-5,5')-(diphenyl)-3,3'-(3,3'-dimethoxy-4,4'-diphenylene) ditetrazolium chloride = HCN + ?
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reduced electron-transferring flavoprotein + nitro blue tetrazolium = electron transferring-flavoprotein + reduced nitro blue tetrazolium
show the reaction diagram
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5-bromo-4-chloro-3-indolyl phosphate + nitroblue tetrazolium = ?
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NADH + nitroblue tetrazolium = NAD+ + reduced nitroblue tetrazolium
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ascorbate[side 1] + nitroblue tetrazolium[side 2] = dehydroascorbate + ?
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Product in Enzyme-catalyzed Reactions (24 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
methanol + nitroblue tetrazolium = formaldehyde + reduced nitroblue tetrazolium
show the reaction diagram
-
-
polyvinyl alcohol + nitro blue tetrazolium = polyvinylketone + reduced nitro blue tetrazolium
show the reaction diagram
-
-
formate + nitrobluetetrazolium = CO2 + reduced nitrobluetetrazolium
show the reaction diagram
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formate + nitroblue tetrazolium = CO2 + reduced nitroblue tetrazolium
show the reaction diagram
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NADH + nitroblue tetrazolium = NAD+ + reduced nitroblue tetrazolium
show the reaction diagram
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oxidized Nitro Blue tetrazolium + NADH = reduced Nitro Blue tetrazolium + NAD+ + H+
show the reaction diagram
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pyruvate + CoA + oxidized nitro blue tetrazolium = acetyl-CoA + CO2 + reduced nitro blue tetrazolium + H+
show the reaction diagram
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reduced electron-transferring flavoprotein + nitro blue tetrazolium = electron transferring-flavoprotein + reduced nitro blue tetrazolium
show the reaction diagram
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NADH + oxidized nitroblue tetrazolium = NAD+ + H+ + reduced nitroblue tetrazolium
show the reaction diagram
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NADH + nitroblue tetrazolium = NAD+ + reduced nitroblue tetrazolium
show the reaction diagram
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Enzyme Cofactor/Cosubstrate (1 result)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activation
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Inhibitor in Enzyme-catalyzed Reactions (10 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
slight inhibition
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (2 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.65
-
NADPH-diaphorase activity
9.55
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cosubstrate xanthine

KM Value (5 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.15
-
at pH 7.0 and 30°C
0.016
-
-
0.0113
-
electron donor: xanthine

Ki Value (2 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.04
-
-
0.007
-
-

References & Links

Links to other databases for nitroblue tetrazolium

ChEBI
PubChem
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PubChem