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Ligand N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.86

UDPgalactose + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide = UDP + 1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide

639628, 639630, 639633

-

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.206

UDP-N-acetyl-D-glucosamine + lactosylceramide = UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

0

-

Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Substrate (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.179

UDP-galactose + lactotriaosylceramide = UDP + lactotetraosylceramide

638379, 638381, 638380

-

2.4.1.275

UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->)-ceramide = ?

715137

-

2.4.1.86

UDPgalactose + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide = UDP + 1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide

639630, 639632, 639628, 639633, 639629

-

Product in Enzyme-catalyzed Reactions (3 results)

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Show Product (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.206

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1-4)-beta-D-glucosyl-(1-1)-ceramide = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide

0

-

2.4.1.206

UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide = UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

638235, 638236, 638234, 638230, 638231, 638232, 638233

-

2.4.1.206

UDP-N-acetyl-D-glucosamine + lactosylceramide = UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide

0

-

2.4.1.206

3 entries

Enzyme Kinetic Parameters

K_M Value (6 results)

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Show KM Value (6 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.1.179

0.013

-

-

638380

2.4.1.275

0.11

-

pH 7.2, 37°C

715137

2.4.1.86

0.034

-

-

639633

2.4.1.86

0.11

-

-

639632

2.4.1.86

0.119

-

-

639630

2.4.1.86

0.5

-

-

639629

2.4.1.86

4 entries

References & Links

Literature References (16)

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Show Reference (16 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

638230

1825612

Glycolipids and glycosyltransferases in permanent cell lines established from human medulloblastomas

1991

Gottfries, J.; Percy, A.K.; Mansson, J.E.; Fredman, P.; Wikstrand, C.J.; Friedman, H.S.; Bigner, D.D.; Svennerholm, L.

Biochim. Biophys. Acta

1081

253-261

638231

2960671

Synthesis of type 1 and 2 lacto series glycolipid antigens in human colonic adenocarcinoma and derived cell lines is due to activation of a normally unexpressed beta 1-3N-acetylglucosaminyltransferase

1987

Holmes, E.H.; Hakomori, S.i.; Ostrander, G.K.

J. Biol. Chem.

262

15649-15658

638232

8408027

N-acetylglucosaminyltransferase regulates the expression of neolactoglycolipids including sulfoglucuronylglycolipids in the developing nervous system

1993

Chou, D.K.H.; Jungalwala, F.B.

J. Biol. Chem.

268

21727-21733

638233

10561117

Glycolipid glycosyltransferase activities during early development of Xenopus: effect of retinoic acid

1999

Rossi, F.; Gornati, R.; Rizzo, A.M.; Venturini, L.; Bernadini, G.; Berra, B.

Cell Biol. Int.

23

91-95

638234

8910533

N-acetylglucosaminyl Transferase regulates the expression of sulfoglucuronyl glycolipids in specific cell types in cerebellum during development

1996

Chou, D.K.H.; Jungalwala, F.B.

J. Biol. Chem.

271

28868-28874

638235

11283017

Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T5), an essential enzyme for the expression of HNK-1 and lewis X epitopes on glycolipids

2001

Togayachi, A.; Akashima, T.; Ookubo, R.; Kudo, T.; Nishihara, S.; Iwasaki, H.; Natsume, A.; Mio, H.; Inokuchi, J.i.; Irimura, T.; Sasaki, K.; Narimatsu, H.

J. Biol. Chem.

276

22032-22040

638236

11384981

Cloning of a mouse beta1,3 N-acetylglucosaminyltransferase GlcNAc(beta1,3)Gal(beta1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis

2001

Henion, T.R.; Zhou, D.; Wolfer, D.P.; Jungalwala, F.B.; Hennet, T.

J. Biol. Chem.

276

30261-30269

638379

2505675

Preparative in vitro generation of lacto-series type 1 chain glycolipids catalyzed by beta 1-3-galactosyltransferase from human colonic adenocarcinoma Colo 205 cells

1989

Holmes, E.H.; Levery, S.B.

Arch. Biochem. Biophys.

274

14-25

638380

2495770

Characterization and membrane organization of beta 1-3- and beta 1-4-galactosyltransferases from human colonic adenocarcinoma cell lines Colo 205 and SW403: basis for preferential synthesis of type 1 chain lacto-series carbohydrate structures

1989

Holmes, E.H.

Arch. Biochem. Biophys.

270

630-646

638381

1429882

Stable expression of a cDNA encoding a human beta1 -> 3galactosyltransferase responsible for lacto-series type 1 core chain synthesis in non-expressing cells: variation in the nature of cell surface antigens expressed

1992

Sherwood, A.L.; Greene, T.G.; Holmes, E.H.

J. Cell. Biochem.

50

165-177

639628

291963

Differential activities of glycolipid glycosyltransferases in Tay-Sachs disease: studies in cultured cells from cerebrum

1979

Basu, M.; Presper, K.A.; Basu, S.; Hoffmann, L.M.; Brooks, S.E.

Proc. Natl. Acad. Sci. USA

76

4270-4274

639629

4335001

Enzymatic synthesis of a tetraglycosylceramide by a galactosyltransferase from rabbit bone marrow

1972

Basu, M.; Basu, S.

J. Biol. Chem.

247

1489-1495

639630

9821875

Purification and characterization of avian glycolipid: beta-galactosyltransferases (GalT-4 and GalT-3): cloning and expression of truncated bGalT-4

1998

Basu, S.S.; Dastgheib, S.; Ghosh, S.; Basu, M.; Kelly, P.; Basu, S.

Acta Biochim. Pol.

45

451-467

639632

8781973

Biosynthesis in vitro of neolactotetraosylceramide by a galactosyltransferase from mouse T-lymphoma: purification and kinetic studies; synthesis of neolacto and polylactosamine core

1996

Basu, M.; Weng, S.A.; Tang, H.; Khan, F.; Rossi, F.; Basu, S.

Glycoconjugate J.

13

423-432

639633

8263530

Characterization and developmental expression of lactotriosylceramide:galactosyltransferase for the synthesis of neolactotetraosylceramide in the nervous system

1994

Chou, D.K.; Jungalwala, F.B.

J. Neurochem.

62

307-314

715137

8781973

Biosynthesis in vitro of neolactotetraosylceramide by a galactosyltransferase from mouse T-lymphoma: purification and kinetic studies; synthesis of neolacto and polylactosamine core

1996

Basu, M.; Weng, S.A.; Tang, H.; Khan, F.; Rossi, F.; Basu, S.

Glycoconj. J.

13

423-432

Links to other databases for N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide

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