Ligand 5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2
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Basic Ligand Information
Molecular Structure

C25H30N8O9
5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2
AHTGOXIPWBOHFC-YVZMLIKISA-N
5,10-methylenetetrahydropteroyldiglutamate
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (1 result)
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ATP + 5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2 + L-glutamate = ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)3
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Product in Enzyme-catalyzed Reactions (1 result)
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tetrahydropteroyldiglutamate + 2-dehydropantoate = 5,10-methylenetetrahydropteroyldiglutamate + 3-methyl-2-oxobutanoate + H2O
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Enzyme Kinetic Parameters
KM Value (2 results)
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0.01
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mutant D151A, pH 9.8
0.204
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mutant R15E, pH 9.8
References & Links
Literature References (1)
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Mutagenesis of folylpolyglutamate synthetase indicates that dihydropteroate and tetrahydrofolate bind to the same site
2008
Sheng, Y.; Khanam, N.; Tsaksis, Y.; Shi, X.M.; Lu, Q.S.; Bognar, A.L.
Biochemistry
47
2388-2396
Links to other databases for 5,10-methylene-5,6,7,8-tetrahydrofolyl-(L-Glu)2