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Ligand GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (5 results)

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Show Substrate (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.145

UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine = ?

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2.4.1.145

UDP-N-acetyl-D-glucosamine + GlcNAcbeta1-2Manalpha1-3(GlcNAcbeta1-2Manalpha1-6)Manbeta1-4GlcNAcbeta1-4GlcNAc-2-aminopyridine = UDP + ?

688325

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2.4.1.145

2 entries

2.4.1.244

UDP-GalNAc + GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside = UDP + GalNAc-beta(1,4)-GlcNAc-beta-(1,2)-Man-alpha-(1,6)-[GlcNAc-beta-(1,2)-Man-alpha-(1,3)]Man-beta-(1,4)-GlcNAc-beta-(1,4)-GlcNAc-2-pyridylaminoside

658735, 669203

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3.2.1.52

GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O = GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + N-acetyl-beta-D-glucosamine

665721

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3.2.1.52

GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + H2O = Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside + N-acetyl-beta-D-glucosamine

665721

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3.2.1.52

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (2 results)

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Show Turnover Number (2 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.52

0.0005

-

product MGn-2-aminopyridine

665721

3.2.1.52

0.0025

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product GnM-2-aminopyridine

665721

3.2.1.52

2 entries

K_M Value (4 results)

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Show KM Value (4 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.1.145

0.971

-

37°C, pH 7.5, recombinant full-length N-acetylglucosaminyltransferase-IVa

673896

2.4.1.145

1.04

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37°C, pH 7.5, recombinant soluble human flag-GnTIVa

673896

2.4.1.145

5.72

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37°C, pH 7.5, recombinant full-length N-acetylglucosaminyltransferase-IVb

673896

2.4.1.145

6.94

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37°C, pH 7.5, recombinant soluble human flag-GnTIVa

673896

2.4.1.145

4 entries

References & Links

Literature References (5)

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Show Reference (5 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

658735

15044014

Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine

2004

Gotoh, M.; Sato, T.; Kiyohara, K.; Kameyama, A.; Kikuchi, N.; Kwon, Y.D.; Ishizuka, Y.; Iwai, T.; Nakanishi, H.; Narimatsu, H.

FEBS Lett.

562

134-140

665721

16684772

Purification, characterization, and cloning of a spodoptera frugiperda SF9 beta-N-acetylhexosaminidase that hydrolyzes terminal N-acetylglucosamine on N-glycan core

2006

Tomiya, N.; Narang, S.; Park, J.; Abdul-Rahman, B.; Choi, O.; Singh, S.; Hiratake, J.; Sakata, S.; Betenbaugh, M.J.; Palter, K.B.; Lee, Y.C.

J. Biol. Chem.

281

19545-19560

669203

12966086

Molecular cloning and characterization of a novel human beta1,4-N-acetylgalactosaminyltransferase, beta4GalNAc-T3, responsible for the synthesis of N,N'-diacetyllactosediamine, GalNAc beta1-4GlcNAc

2003

Sato, T.; Gotoh, M.; Kiyohara, K.; Kameyama, A.; Kubota, T.; Kikuchi, N.; Ishizuka, Y.; Iwasaki, H.; Togayachi, A.; Kudo, T.; Ohkura, T.; Nakanishi, H.; Narimatsu, H.

J. Biol. Chem.

278

47534-47544

673896

17006639

Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes

2006

Oguri, S.; Yoshida, A.; Minowa, M.T.; Takeuchi, M.

Glycoconj. J.

23

473-480

688325

18309288

Expression and characterization of human N-acetylglucosaminyltransferases and alpha2,3-sialyltransferase in insect cells for in vitro glycosylation of recombinant erythropoietin

2008

Kim, N.Y.; Kim, H.G.; Kim, Y.H.; Chung, I.S.; Yang, J.M.

J. Microbiol. Biotechnol.

18

383-391

Links to other databases for GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-pyridylaminoside

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ChEBI

PubChem

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