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Ligand Na+
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Basic Ligand Information
Na+/[side 1], Na+/[side 2], Na+in, Na+ in, Na+out, Na+ out, Na+out (n = 1-2), Na+[side 1], Na+[side 2], Na/+in, Na/+out, sodium
KEGG
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open all tablesRoles as Enzyme Ligand
In Vivo Substrate in Enzyme-catalyzed Reactions (37 results)
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EC NUMBER 
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
deamino-NADH + H+ + ubiquinone + 2 Na+/in = deamino-NAD+ + ubiquinol + 2 Na+/out
deamino-NADH + H+ + ubiquinone + n Na+/in = deamino-NAD+ + ubiquinol + n Na+/out
NADH + H+ + ubiquinone + 2 Na+/in = NAD+ + ubiquinol + 2 Na+/out
NADH + H+ + ubiquinone + n Na+/in = NAD+ + ubiquinol + n Na+/out
thio-NADH + H+ + ubiquinone + 2 Na+/in = thio-NAD+ + ubiquinol + 2 Na+/out
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in = 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
2 reduced ferredoxin [iron-sulfur] cluster + methanophenazine + Na+[side 1] = 2 oxidized ferredoxin [iron-sulfur] cluster + reduced methanophenazine + Na+[side 2]
-
2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+[side 1] = 2 oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+[side 2]
-
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1] = oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
ATP + H2O + Na+/in = ADP + phosphate + Na+/out
-
ADP + phosphate + Na+/out = ATP + H2O + Na+/in
ADP + phosphate + Na+/out = ATP + H2O + Na+/in
ADP + phosphate + Na+/out = ATP + H2O + Na+/in
ATP + H2O + Na+/in + K+/out = ADP + phosphate + Na+/out + K+/in
735014, 713011, 735173, 712794, 711156, 711774, 712134, 712538, 712805, 734675, 710811, 713007, 713111, 733517, 734325, 734529, 734530, 734538, 735119, 735146, 735302, 710778, 711155, 734421, 734774, 711739, 734355, 734580, 734356, 734461, 733681, 713398, 735190, 734462, 735008, 733313, 735111, 246955, 246960, 246967, 713119
ATP + H2O + Na+/in + K+/out = ADP + phosphate + Na+/out + K+/in
735014, 713011, 735173, 712794, 711156, 711774, 712134, 712538, 712805, 734675, 710811, 713007, 713111, 733517, 734325, 734529, 734530, 734538, 735119, 735146, 735302, 710778, 711155, 734421, 734774, 711739, 734355, 734580, 734356, 734461, 733681, 713398, 735190, 734462, 735008, 733313, 735111, 246955, 246960, 246967, 713119
ATP + H2O + Na+/in + K+/out = ADP + phosphate + Na+/out + K+/in
735014, 713011, 735173, 712794, 711156, 711774, 712134, 712538, 712805, 734675, 710811, 713007, 713111, 733517, 734325, 734529, 734530, 734538, 735119, 735146, 735302, 710778, 711155, 734421, 734774, 711739, 734355, 734580, 734356, 734461, 733681, 713398, 735190, 734462, 735008, 733313, 735111, 246955, 246960, 246967, 713119
ATP + H2O + 3 Na+/in + 2 K+/out = ADP + phosphate + 3 Na+/out + 2 K+/in
ATP + H2O + 3 Na+/in + 2 K+/out = ADP + phosphate + 3 Na+/out + 2 K+/in
ATP + H2O + 3 Na+/in + 2 K+/out = ADP + phosphate + 3 Na+/out + 2 K+/in
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
ATP + H2O + Na+/in = ADP + phosphate + Na+/out
711297, 711004, 711011, 711013, 711296, 713191, 210468, 210464, 210458, 210459, 210271, 210276, 685317
-
ATP + H2O + Na+[side 1] = ADP + phosphate + Na+[side 2]
-
(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] = propanoyl-CoA + CO2 + Na+[side 2]
-
glutaconyl-CoA + Na+[side 1] = but-2-enoyl-CoA + CO2 + Na+[side 2]
-
In Vivo Product in Enzyme-catalyzed Reactions (34 results)
EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
deamino-NADH + H+ + ubiquinone + 2 Na+/in = deamino-NAD+ + ubiquinol + 2 Na+/out
-
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in = 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
-
2 reduced ferredoxin [iron-sulfur] cluster + methanophenazine + Na+[side 1] = 2 oxidized ferredoxin [iron-sulfur] cluster + reduced methanophenazine + Na+[side 2]
-
-
2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+[side 1] = 2 oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+[side 2]
-
-
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1] = oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
-
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
-
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
-
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
-
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
-
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
-
ATP + H2O + Na+/in = ADP + phosphate + Na+/out
-
Substrate in Enzyme-catalyzed Reactions (77 results)
EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
deamino-NADH + H+ + ubiquinone + 2 Na+/in = deamino-NAD+ + ubiquinol + 2 Na+/out
deamino-NADH + H+ + ubiquinone + n Na+/in = deamino-NAD+ + ubiquinol + n Na+/out
NADH + H+ + ubiquinone + 2 Na+/in = NAD+ + ubiquinol + 2 Na+/out
NADH + H+ + ubiquinone + n Na+/in = NAD+ + ubiquinol + n Na+/out
NADH + H+ + ubiquinone-1 + n Na+/in = NAD+ + ubiquinol-1 + n Na+/out
thio-NADH + H+ + ubiquinone + 2 Na+/in = thio-NAD+ + ubiquinol + 2 Na+/out
2 ferricyanide + NADH + n Na+/in = 2 ferrocyanide + NAD+ + H+ + n Na+/out
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in = 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
2 reduced ferredoxin [iron-sulfur] cluster + methanophenazine + Na+[side 1] = 2 oxidized ferredoxin [iron-sulfur] cluster + reduced methanophenazine + Na+[side 2]
-
2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+[side 1] = 2 oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+[side 2]
-
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1] = oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1] = oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
ATP + H2O + Na+/in = ADP + phosphate + Na+/out
-
ADP + phosphate + Na+/out = ATP + H2O + Na+/in
ADP + phosphate + Na+/out = ATP + H2O + Na+/in
ADP + phosphate + Na+/out = ATP + H2O + Na+/in
ATP + H2O + Na+/in + K+/out = ADP + phosphate + Na+/out + K+/in
246966, 246969, 735014, 246962, 713011, 735173, 246961, 246953, 712794, 246952, 246960, 246968, 246973, 711156, 711774, 712134, 712538, 712805, 734675, 246950, 246954, 246958, 246959, 246964, 246972, 695469, 695495, 710811, 713007, 713111, 713119, 733517, 734325, 734529, 734530, 734538, 735119, 735146, 735302, 246965, 246971, 695408, 710778, 711155, 734421, 734774, 246963, 700145, 246970, 711739, 246957, 734355, 246967, 246951, 246974, 734580, 734356, 246955, 734461, 697455, 733681, 713398, 735190, 734462, 735008, 733313, 735111, 246949, 246956, 246948
ATP + H2O + Na+/in + K+/out = ADP + phosphate + Na+/out + K+/in
246966, 246969, 735014, 246962, 713011, 735173, 246961, 246953, 712794, 246952, 246960, 246968, 246973, 711156, 711774, 712134, 712538, 712805, 734675, 246950, 246954, 246958, 246959, 246964, 246972, 695469, 695495, 710811, 713007, 713111, 713119, 733517, 734325, 734529, 734530, 734538, 735119, 735146, 735302, 246965, 246971, 695408, 710778, 711155, 734421, 734774, 246963, 700145, 246970, 711739, 246957, 734355, 246967, 246951, 246974, 734580, 734356, 246955, 734461, 697455, 733681, 713398, 735190, 734462, 735008, 733313, 735111, 246949, 246956, 246948
ATP + H2O + Na+/in + K+/out = ADP + phosphate + Na+/out + K+/in
246966, 246969, 735014, 246962, 713011, 735173, 246961, 246953, 712794, 246952, 246960, 246968, 246973, 711156, 711774, 712134, 712538, 712805, 734675, 246950, 246954, 246958, 246959, 246964, 246972, 695469, 695495, 710811, 713007, 713111, 713119, 733517, 734325, 734529, 734530, 734538, 735119, 735146, 735302, 246965, 246971, 695408, 710778, 711155, 734421, 734774, 246963, 700145, 246970, 711739, 246957, 734355, 246967, 246951, 246974, 734580, 734356, 246955, 734461, 697455, 733681, 713398, 735190, 734462, 735008, 733313, 735111, 246949, 246956, 246948
ATP + 3 Na+/in = ADP + phosphate + 3 Na+/out
ATP + 3 Na+/in = ADP + phosphate + 3 Na+/out
ATP + 3 Na+/in = ADP + phosphate + 3 Na+/out
ATP + H2O + 3 Na+/in + 2 K+/out = ADP + phosphate + 3 Na+/out + 2 K+/in
ATP + H2O + 3 Na+/in + 2 K+/out = ADP + phosphate + 3 Na+/out + 2 K+/in
ATP + H2O + 3 Na+/in + 2 K+/out = ADP + phosphate + 3 Na+/out + 2 K+/in
ATP + H2O + Na+/in + Rb+/out = ADP + phosphate + Na+/out + Rb+/in
ATP + H2O + Na+/in + Rb+/out = ADP + phosphate + Na+/out + Rb+/in
ATP + H2O + Na+/in + Rb+/out = ADP + phosphate + Na+/out + Rb+/in
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
ATP + H2O + Na+[side 1] + Rb+[side 2] = ADP + phosphate + Na+[side 2] + Rb+[side 1]
ATP + H2O + Na+[side 1] + Rb+[side 2] = ADP + phosphate + Na+[side 2] + Rb+[side 1]
ATP + H2O + Na+[side 1] + Rb+[side 2] = ADP + phosphate + Na+[side 2] + Rb+[side 1]
ATP + H2O + Na+/in = ADP + phosphate + Na+/out
711297, 701427, 711004, 711011, 711013, 711296, 713191, 718694, 656725, 210468, 210464, 655506, 695869, 210458, 210459, 210271, 210276, 655394, 655392, 685317, 719531, 718973, 719166, 719727
-
ATP + H2O + Na+[side 1] = ADP + phosphate + Na+[side 2]
-
(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] = propanoyl-CoA + CO2 + Na+[side 2]
-
4-carboxybut-2-enoyl-CoA + Na+[side 1] = but-2-enoyl-CoA + CO2 + Na+[side 2]
-
glutaconyl-CoA + Na+[side 1] = but-2-enoyl-CoA + CO2 + Na+[side 2]
-
(2E)-4-carboxybut-2-enoyl-CoA + Na+[side 1] = (2E)-but-2-enoyl-CoA + CO2 + Na+[side 2]
-
Product in Enzyme-catalyzed Reactions (78 results)
EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ATP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 = ADP + phosphate + dethiobiotin + Na+ + OH-
-
CTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 = CDP + phosphate + dethiobiotin + Na+ + OH-
-
GTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 = GDP + phosphate + dethiobiotin + Na+ + OH-
-
ITP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 = IDP + phosphate + dethiobiotin + Na+ + OH-
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TTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 = TDP + phosphate + dethiobiotin + Na+ + OH-
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UTP + (7R,8S)-7,8-diaminopelargonic acid + NaHCO3 = UDP + phosphate + dethiobiotin + Na+ + OH-
-
deamino-NADH + H+ + ubiquinone + 2 Na+/in = deamino-NAD+ + ubiquinol + 2 Na+/out
-
2 reduced ferredoxin iron-sulfur cluster + NAD+ + H+ + Na+/in = 2 oxidized ferredoxin iron-sulfur cluster + NADH + Na+/out
-
-
2 reduced ferredoxin [iron-sulfur] cluster + methanophenazine + Na+[side 1] = 2 oxidized ferredoxin [iron-sulfur] cluster + reduced methanophenazine + Na+[side 2]
-
-
2 reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+[side 1] = 2 oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+[side 2]
-
-
reduced ferredoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1] = oxidized ferredoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
reduced flavodoxin [iron-sulfur] cluster + NAD+ + H+ + Na+/[side 1] = oxidized flavodoxin [iron-sulfur] cluster + NADH + Na+/[side 2]
-
-
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
-
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
-
ATP + H2O + 3 Na+[side 1] + 2 K+[side 2] = ADP + phosphate + 3 Na+[side 2] + 2 K+[side 1]
-
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
-
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
-
ATP + H2O + Na+[side 1] + K+[side 2] = ADP + phosphate + Na+[side 2] + K+[side 1]
-
ATP + H2O + Na+[side 1] + Rb+[side 2] = ADP + phosphate + Na+[side 2] + Rb+[side 1]
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ATP + H2O + Na+[side 1] + Rb+[side 2] = ADP + phosphate + Na+[side 2] + Rb+[side 1]
-
ATP + H2O + Na+[side 1] + Rb+[side 2] = ADP + phosphate + Na+[side 2] + Rb+[side 1]
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ATP + H2O + Na+/in = ADP + phosphate + Na+/out
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(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] = propanoyl-CoA + CO2 + Na+[side 2]
-
Activator in Enzyme-catalyzed Reactions (1043 results)
EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
wild-type, no activation, engineering of wild-type into a Na+-activated enzyme by replacing residues in the 170, 186, and 220 loops to those of coagulation factor Xa
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
binding of Na+ serves to stabilize the enzyme in a more open and rigid conformation. Na+-bound enzyme is more stable to denaturation by urea and more resistant to limited proteolysis by subtilisin
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
in Na+-free form, enzyme is in an equilibrium between an inert species and an active form, where the addition of Na+ populates the active state
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
increase in ratio kcat/KM from 0.0018 per mM and s in absence of Na+ to 0.125 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
not activating in wild-type. Mutant K222D, increase in ratio kcat/KM from 0.039 per mM and s in absence of Na+ to 0.069 per mM and s in presence of saturating concentration of Na+
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
bound by Tyr225 of the wild-type enzyme, stimulation of amidolytic activity cleaving small synthetic substrates, kinetics, temperature dependence of dissociation constants in absence and presence of Ca2+, mutant GDFXa Y225P is 2fold less stimulatable, can only partly be replaced by other monovalent cations
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-aminoacrylate Schiff base at the beta-site
wild-type enzyme alpha-site is activated by the formation of the alpha-