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Ligand 4-nitrotoluene

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (6 results)

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Show Substrate (6 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.11.2.1

4-nitrotoluene + H2O2 = 4-nitrobenzyl alcohol + H2O

711105

-

1.14.12.23

4-nitrotoluene + NADH + O2 = 4-methylcatechol + nitrite + NAD+

733176, 733150

-

1.14.12.24

4-nitrotoluene + NADH + H+ + O2 = 4-nitrobenzyl alcohol + NAD+ + ?

734055

-

1.14.12.24

4-nitrotoluene + NADH + O2 = 4-methylcatechol + nitrite + NAD+

733548

-

1.14.12.24

2 entries

1.5.1.30

4-nitrotoluene + NADH + H+ = ? + NAD+

699057

-

1.7.1.16

4-nitrotoluene + 2 NADPH + 2 H+ = N-hydroxy-4-methylaniline + 2 NADP+ + H2O

740989

-

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

1.11.2.1

the low extent of 4-nitrotoluene oxidation is attributable to inhibition of the enzyme by the substrate

711105

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Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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Show Turnover Number (1 entry)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.7.1.16

0.13

-

pH 8.0, 37°C

740989

K_M Value (1 result)

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Show KM Value (1 entry)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.7.1.16

0.282

-

pH 8.0, 37°C

740989

References & Links

Literature References (7)

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Show Reference (7 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

699057

19147107

Characterization of a flavin reductase from a thermophilic dibenzothiophene-desulfurizing bacterium, Bacillus subtilis WU-S2B

2009

Takahashi, S.; Furuya, T.; Ishii, Y.; Kino, K.; Kirimura, K.

J. Biosci. Bioeng.

107

38-41

711105

20470751

Stepwise oxygenations of toluene and 4-nitrotoluene by a fungal peroxygenase

2010

Kinne, M.; Zeisig, C.; Ullrich, R.; Kayser, G.; Hammel, K.E.; Hofrichter, M.

Biochem. Biophys. Res. Commun.

397

18-21

733150

11823201

Molecular characterization and substrate specificity of nitrobenzene dioxygenase from Comamonas sp. strain JS765

2002

Lessner, D.; Johnson, G.; Parales, R.; Spain, J.; Gibson, D.

Appl. Environ. Microbiol.

68

634-641

733176

25344236

Selection for growth on 3-nitrotoluene by 2-nitrotoluene-utilizing Acidovorax sp. strain JS42 identifies nitroarene dioxygenases with altered specificities

2015

Mahan, K.M.; Penrod, J.T.; Ju, K.S.; Al Kass, N.; Tan, W.A.; Truong, R.; Parales, J.V.; Parales, R.E.

Appl. Environ. Microbiol.

81

309-319

733548

16028295

Saturation mutagenesis of 2,4-DNT dioxygenase of Burkholderia sp. strain DNT for enhanced dinitrotoluene degradation

2005

Leungsakul, T.; Keenan, B.G.; Yin, H.; Smets, B.F.; Wood, T.K.

Biotechnol. Bioeng.

92

416-426

734055

8759857

2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase

1996

Suen, W.C.; Haigler, B.E.; Spain, J.C.

J. Bacteriol.

178

4926-4934

740989

27138989

Heterologous overexpression and biochemical characterization of a nitroreductase from Gluconobacter oxydans 621H

2016

Yang, Y.; Lin, J.; Wei, D.

Mol. Biotechnol.

58

428-440

Links to other databases for 4-nitrotoluene

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Show Links (1 entry)

ChEBI

PubChem