Ligand azide

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Basic Ligand Information

Molecular Structure
Picture of azide (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
HN3
azide
JUINSXZKUKVTMD-UHFFFAOYSA-N

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
azide + ATP + H2O = ?
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
beta-D-galactosyl azide + H2O = azide + beta-D-galactose
show the reaction diagram
-
-
L-leucine hydrazide + H2O = L-leucine + azide
show the reaction diagram
-
-

Activator in Enzyme-catalyzed Reactions (6 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
1 mM, 30-40% increase in activity, 2,6-dichlorophenol as electron acceptor
-
at pH 4.0, infra-micromolar concentrations of azide enhance enzyme activity
-
0.005 M
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50 mM-0.5 M, stimulation of mutant enzyme E451A
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increases the rate of substrate cleavage in depending of azide concentration
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1 mM, 125% of initial activity
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Inhibitor in Enzyme-catalyzed Reactions (178 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
5 mM, 50% inhibition
-
typical inhibitors of catecholoxidase, also inhibit the phenoloxidase activity of activated hemocyanin
-
75 mM, 50% inhibition
-
weak, binds to the six-coordinate resting enzyme to form a new six-coordinate Fe2+ species
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9% inhibition at 1 mM
-
12 mM, 50% inhibition
-
weak
-
competitive inhibitor
-
0.3 mM NaN3, about 80% inhibition
-
slight inhibition
-
previously reported, not confirmed
-
36% inhibition at 2 mM
-
binding mode, overview
-
50% inhibition at 3.2 mM
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5 mM, 95-98% inhibition
-
weak inhibition of wild-type activity
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92% inhibition at 10 mM
-
weak
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complete inhibition at the ionic strength of 2 mM
-
inhibitory at 20 mM
-
slight inhibition
-
2 mM, 20% inhibition
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the translocation step across thylakoid membranes is inhibited by the SecA inhibitor azide
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1.5 mM azide decrease the transport of the cell-surface glycoprotein mutant GspB736flag, 15 mM nearly prevent transport of GspB736flag, azide have no effect on ATP hydrolysis activity of the recombinant protein 6His-SecA; 1.5 mM azide markedly decrease the transport of the cell-surface glycoprotein mutant GspB736flag, 15 mM nearly prevent transport of GspB736flag, azide have no effect on ATP hydrolysis activity of the recombinant protein 6His-SecA2
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Enzyme Kinetic Parameters

Ki Value (33 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
1.33
-
-
0.051
-
in 67 mM phosphate buffer, pH 7.0, at 30°C
0.001
0.008
depending on substrate
0.5
-
-
0.039
-
pH 7.5, temperature not specified in the publication
0.044
-
at low concentration
2
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recombinant enzyme, pH 6.5, 25°C

IC50 Value (38 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.82
-
pH 6.5, 25°C

References & Links

Links to other databases for azide

ChEBI
PubChem
ChEBI
PubChem