Ligand N-formimino-L-glutamate

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Basic Ligand Information

Molecular Structure
Picture of N-formimino-L-glutamate (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C6H10N2O4
N-formimino-L-glutamate
NRXIKWMTVXPVEF-BYPYZUCNSA-N
Synonyms:
formimino-L-glutamic acid, formiminoglutamate, formiminoglutamic acid, N-formimidoyl-L-glutamate, N-formimino-L-glutamic acid, N-formiminoglutamate, N-forminio-L-glutamate


Show all pahtways known for Show all BRENDA pathways known for N-formimino-L-glutamate

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (6 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE

In Vivo Product in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
5-formimidoyltetrahydrofolate + L-glutamate = tetrahydrofolate + N-formimidoyl-L-glutamate
-
-
show the reaction diagram
(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+

Substrate in Enzyme-catalyzed Reactions (12 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE

Product in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
5-formimidoyltetrahydrofolate + L-glutamate = tetrahydrofolate + N-formimidoyl-L-glutamate
-

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (16 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
Cd2+-reconstituted wild type enzyme, at pH 8.0 and 30°C
31
-
Cu2+-reconstituted wild type enzyme, at pH 8.0 and 30°C
8.5
-
mutant enzyme E235A, at pH 8.0 and 30°C
0.1
-
mutant enzyme E235D, at pH 8.0 and 30°C
0.005
-
mutant enzyme E235Q, at pH 8.0 and 30°C
0.12
-
Ni2+-reconstituted wild type enzyme, at pH 8.0 and 30°C
21.3
-
pH 8.0, 30°C
13.2
-
Zn2+ has been reconstituted with Cd2+, pH 8.0, 30°C
31
-
Zn2+ has been reconstituted with Cu2+, pH 8.0, 30°C
8.5
-
Zn2+ has been reconstituted with Ni2+, pH 8.0, 30°C
21.3
-
Zn2+-reconstituted wild type enzyme, at pH 8.0 and 30°C
13.2
-
mutant enzyme N114H, at pH 8.5 and 22°C
80
-
mutant enzyme R144A, at pH 8.5 and 22°C
3.2
-
mutant enzyme R144E, at pH 8.5 and 22°C
0.05
-
mutant enzyme R144K, at pH 8.5 and 22°C
190
-
wild type enzyme, at pH 8.5 and 22°C
200
-

KM Value (26 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
-
11
-
wild-type bifunctional formiminotransferase cyclodeaminase
5.8
-
tetrahydropteroic acid as substrate
2
-
tetrahydrofolate as substrate
12
-
recombinant His-tagged formiminotransferase domain
8.3
-
recombinant His-tagged bifunctional formiminotransferase cyclodeaminase
6.7
-
-
0.1
-
Zn2+ has been reconstituted with Ni2+, pH 8.0, 30°C
0.0027
-
Zn2+ has been reconstituted with Cu2+, pH 8.0, 30°C
0.0012
-
Zn2+ has been reconstituted with Cd2+, pH 8.0, 30°C
0.0021
-
wild-type, pH 8.0, 30°C
0.22
-
wild type enzyme, at pH 8.0 and 30°C
0.22
-
Ni2+-reconstituted wild type enzyme, at pH 8.0 and 30°C
2.7
-
mutant enzyme E235Q, at pH 8.0 and 30°C
19
-
mutant enzyme E235D, at pH 8.0 and 30°C
0.66
-
mutant enzyme E235A, at pH 8.0 and 30°C
2.2
-
Cu2+-reconstituted wild type enzyme, at pH 8.0 and 30°C
1.2
-
Cd2+-reconstituted wild type enzyme, at pH 8.0 and 30°C
2.1
-
pH 7.4
39
-
pH 7.8
530
-
mutant enzyme R144K, at pH 8.5 and 22°C
160
-
mutant enzyme R144E, at pH 8.5 and 22°C
9
-
mutant enzyme R144A, at pH 8.5 and 22°C
90
-
mutant enzyme N114H, at pH 8.5 and 22°C
20
-
wild type enzyme, at pH 8.5 and 22°C
40
-
-
14
-

References & Links

Links to other databases for N-formimino-L-glutamate

ChEBI
PubChem
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PubChem