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Ligand 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C8 H7 NO4
5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate
JTWNWNJMNSJYDL-UHFFFAOYSA-N
Roles as Enzyme Ligand
In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)
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5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O = 3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
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In Vivo Product in Enzyme-catalyzed Reactions (2 results)
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4-pyridoxate + 2,6-dichloroindophenol = 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol
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4-pyridoxate + NAD+ = 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+
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Substrate in Enzyme-catalyzed Reactions (2 results)
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5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+ = 4-pyridoxate + NAD+
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5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NAD+ + H2O = 3-hydroxy-2-methylpyridine-4,5-dicarboxylate + NADH + H+
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Product in Enzyme-catalyzed Reactions (3 results)
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4-pyridoxate + 2,6-dichloroindophenol = 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced 2,6-dichloroindophenol
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4-pyridoxate + NAD+ = 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + NADH + H+
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4-pyridoxate + phenazine methosulfate = 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate + reduced phenazine methosulfate
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (6 results)
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78
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mutant E149Q, pH 8.0, 30°C
217
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wild-type, pH 8.0, 30°C
300
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mutant E149Q, pH 5.0, 30°C
25
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mutant E149Q, pH 8.0, 30°C
204
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wild-type, pH 8.0, 30°C
400
670
pH 8.0, temperature not specified in the publication
KM Value (6 results)
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0.02
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mutant E149Q, pH 8.0, 30°C
0.0249
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wild-type, pH 8.0, 30°C
0.03
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mutant E149Q, pH 5.0, 30°C
0.0428
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wild-type, pH 8.0, 30°C
0.1
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pH 8.0, temperature not specified in the publication
0.241
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mutant E149Q, pH 8.0, 30°C
References & Links Literature References (3)
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Crystal structure of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase from Mesorhizobium loti
2015
Mugo, A.N.; Kobayashi, J.; Mikami, B.; Yoshikane, Y.; Yagi, T.; Ohnishi, K.
Biochem. Biophys. Res. Commun.
456
35-40
Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase
2009
Yokochi, N.; Yoshikane, Y.; Matsumoto, S.; Fujisawa, M.; Ohnishi, K.; Yagi, T.
J. Biochem.
145
493-503
Enzymes of vitamin B6 degradation. Purification and properties of isopyridoxal dehydrogenase and 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylic-acid dehydrogenase
1986
Lee, Y.; Nelson, M.; Snell, E.
J. Biol. Chem.
261
15106-15111
Links to other databases for 5-formyl-3-hydroxy-2-methylpyridine-4-carboxylate