Ligand 4-amino-2,6-dinitrotoluene
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Basic Ligand Information
Molecular Structure
C7H7N3O4
4-amino-2,6-dinitrotoluene
KQRJATLINVYHEZ-UHFFFAOYSA-N
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (2 results)
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4-amino-2,6-dinitrotoluene + NADH + H+ = 2-hydroxyamino-4-aminotoluene + NAD+ + H2O
-
4-amino-2,6-dinitrotoluene + NADPH + H+ = ? + NADP+ + H2O
-
Enzyme Kinetic Parameters
kcat Value (Turnover Number) (9 results)
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0.76
-
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
3.25
-
pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
4.74
-
pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
8.06
-
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
9.4
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pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
11.29
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pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
11.48
-
pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
14.01
-
pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
1.05
-
pH 7.0, 37°C, recombinant His6-tagged enzyme
KM Value (9 results)
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0.172
-
pH 7.0, 37°C, recombinant His6-tagged mutant N71S, with NADH
0.265
-
pH 7.0, 37°C, recombinant His6-tagged mutant F123A/F124W, with NADH
0.306
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pH 7.0, 37°C, recombinant His6-tagged mutant F124W, with NADH
0.38
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pH 7.0, 37°C, recombinant His6-tagged mutant F123A, with NADH
0.518
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pH 7.0, 37°C, recombinant His6-tagged wild-type enzyme, with NADH
0.732
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pH 7.0, 37°C, recombinant His6-tagged mutant F124A, with NADH
1.069
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pH 7.0, 37°C, recombinant His6-tagged mutant F124N, with NADH
1.168
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pH 7.0, 37°C, recombinant His6-tagged mutant T41L, with NADH
0.6427
-
pH 7.0, 37°C, recombinant His6-tagged enzyme
References & Links
Literature References (1)
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Transformation pathway of 2,4,6-trinitrotoluene by Escherichia coli nitroreductases and improvement of activity using structure-based mutagenesis
2015
Bai, J.; Yang, J.; Liu, P.; Yang, Q.
Process Biochem.
50
705-711
Links to other databases for 4-amino-2,6-dinitrotoluene
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