Ligand 1,2-dihydroxy-3-oxo-1-hexene
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Basic Ligand Information
Molecular Structure

C6H10O3
1,2-dihydroxy-3-oxo-1-hexene
ZMSYAHMNCHZGFQ-XQRVVYSFSA-N
(1Z)-1,2-dihydroxyhex-1-en-3-one, 1,2-dihydroxy-3-oxo-hex-1-ene
Roles as Enzyme Ligand
In Vivo Product in Enzyme-catalyzed Reactions (2 results)
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2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O = 1,2-dihydroxy-3-oxo-1-hexene + phosphate
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2,3-dioxohexyl phosphate + H2O = 1,2-dihydroxy-3-oxo-hex-1-ene + phosphate
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Substrate in Enzyme-catalyzed Reactions (2 results)
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(1Z)-1,2-dihydroxyhex-1-en-3-one + O2 = n-butanoic acid + formic acid + CO
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(1Z)-1,2-dihydroxyhex-1-en-3-one + O2 = 2-oxovalerate + formic acid
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Product in Enzyme-catalyzed Reactions (2 results)
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2-hydroxy-3-oxo-hex-1-enyl phosphate + H2O = 1,2-dihydroxy-3-oxo-1-hexene + phosphate
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2,3-dioxohexyl phosphate + H2O = 1,2-dihydroxy-3-oxo-hex-1-ene + phosphate
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (3 results)
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7.87
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Co2+ bound enzyme, pH 7.0, 25°C
17.1
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Ni2+ bound enzyme, pH 7.0, 25°C
111.7
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Fe2+ bound enzyme, pH 7.0, 25°C
KM Value (3 results)
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0.0493
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Co2+ bound enzyme, pH 7.0, 25°C
0.2871
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Ni2+ bound enzyme, pH 7.0, 25°C
0.1185
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Fe2+ bound enzyme, pH 7.0, 25°C
References & Links
Literature References (2)
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Metal-dependent function of a mammalian acireductone dioxygenase
2016
Deshpande, A.R.; Wagenpfeil, K.; Pochapsky, T.C.; Petsko, G.A.; Ringe, D.
Biochemistry
55
1398-1407
Dual chemistry catalyzed by human acireductone dioxygenase
2017
Deshpande, A.R.; Pochapsky, T.C.; Petsko, G.A.; Ringe, D.
Protein Eng. Des. Sel.
30
197-204
Links to other databases for 1,2-dihydroxy-3-oxo-1-hexene