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Ligand dGTP

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Basic Ligand Information

Molecular Structure
Picture of dGTP (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
Molfile
C10H16N5O13P3
dGTP
HAAZLUGHYHWQIW-KVQBGUIXSA-N
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Synonyms:
2'-deoxy-GTP, 2'-deoxy-L-GTP, 2'-deoxyguanosine 5'-triphosphate, 2'-deoxyguanosine triphosphate, 2'-dGTP, 5'-dGTP, deoxyGTP, deoxyguanosine triphosphate

Related pathways

Pathway Source
Pathways
BRENDA
purine metabolism
KEGG
Nucleotide metabolism, Purine metabolism
MetaCyc
8-oxo-(d)GTP detoxification I, 8-oxo-(d)GTP detoxification II, dZTP biosynthesis, guanosine deoxyribonucleotides de novo biosynthesis I, guanosine deoxyribonucleotides de novo biosynthesis II more


Show all pahtways known for Show all BRENDA pathways known for dGTP

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (12 results)

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EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
dGTP + H2O = deoxyguanosine + triphosphate
show the reaction diagram
-
dGTP + H2O = dGDP + phosphate
show the reaction diagram
-
dGTP + H2O = dGMP + diphosphate
show the reaction diagram
-
wound dsDNA + dGTP + H2O = unwound ssDNA + dGDP + phosphate
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (11 results)

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EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
GTP + thioredoxin = dGTP + thioredoxin disulfide + H2O
show the reaction diagram
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dGDP + (phosphate)n+1 = dGTP + (phosphate)n
show the reaction diagram
-
ATP + dGDP = ADP + dGTP
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (483 results)

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EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
S-adenosyl-L-methionine + dGTP = S-adenosyl-L-homocysteine + m7dGTP
show the reaction diagram
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dGTP + D-ribose = dGDP + D-ribose 5-phosphate
show the reaction diagram
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dGTP + adenosylcobinamide = adenosylcobinamide phosphate + dGDP
show the reaction diagram
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dGTP + adenosine = dGDP + AMP
show the reaction diagram
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dGTP + thymidine = dGDP + dTMP
show the reaction diagram
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dGTP + 2-deoxy-D-ribose = dGDP + 2-deoxy-D-ribose 5-phosphate
show the reaction diagram
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dGTP + NAD+ = dGDP + NADP+
show the reaction diagram
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pantothenate + dGTP = 4'-phosphopantothenate + dGDP
show the reaction diagram
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dGTP + mevalonate = dGDP + phosphomevalonate
show the reaction diagram
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dGTP + uridine = dGDP + UMP
show the reaction diagram
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dGTP + 4-methyl-5-(2-hydroxyethyl)thiazole = dGDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
show the reaction diagram
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dGTP + D-galactose = dGDP + alpha-D-galactose 1-phosphate
show the reaction diagram
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dGTP + dAMP = dGDP + dADP
show the reaction diagram
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dGTP + AMP = dGDP + ADP
show the reaction diagram
-
dGTP + dTMP = dGDP + dTDP
show the reaction diagram
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dGTP + UMP = dGDP + UDP
show the reaction diagram
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ATP + dGTP = diphosphate + ?
show the reaction diagram
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dGTP + dGTP = P1,P4-bis(5'-(2'-deoxyguanosyl) tetraphosphate + diphosphate
show the reaction diagram
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dGTP + tobramycin = diphosphate + 2''-deoxyguanosylyltobramycin
show the reaction diagram
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dGTP + pp(5')RNA = dG(5')ppp(5')RNA + diphosphate
show the reaction diagram
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dGTP + RNAn = diphosphate + RNAn+1
show the reaction diagram
(2S)-2-phospholactate + dGTP = (2S)-lactyl-2-diphospho-5'-(2'-deoxy)guanosine + diphosphate
show the reaction diagram
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p-tRNAHis + ATP + dGTP = ?
show the reaction diagram
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dGTP + alpha-D-glucose 1-phosphate = diphosphate + dGDP-alpha-D-glucose
show the reaction diagram
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dGTP + H2O = deoxyguanosine + triphosphate
show the reaction diagram
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dGTP + dihydrouracil + H2O = ?
show the reaction diagram
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dGTP + H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-deoxyribosylamino)pyrimidine + diphosphate
show the reaction diagram
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dGTP + H2O = 2-amino-5-formylamino-6-hydroxy-4-(deoxy-5-phosphoribosylamino)-pyrimidine + phosphate
show the reaction diagram
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dGTP + H2O = dGDP + phosphate
show the reaction diagram
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dGTP + H2O = ?
show the reaction diagram
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dGTP + H2O = dGMP + diphosphate
show the reaction diagram
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dGTP + H2O = dGMP + diphosphate
show the reaction diagram
-
dGTP + H2O = dGMP + diphosphate
show the reaction diagram
-
dGTP + H2O = dGMP + diphosphate
show the reaction diagram
-
dGTP + H2O = dGMP + diphosphate
show the reaction diagram
-
2'-deoxy-GTP = ?
show the reaction diagram
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dGTP + H2O + closed Cl- channel = dGDP + phosphate + open Cl- channel
show the reaction diagram
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dGTP + H2O = dGDP + phosphate
show the reaction diagram
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dGTP + H2O + wound DNA = dGDP + phosphate + unwound DNA
show the reaction diagram
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dGTP + aminopterin + Glu = dGDP + phosphate + aminopteryl-Glu
show the reaction diagram
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dGTP + L-glutamate + L-cysteine = dGDP + phosphate + gamma-L-glutamyl-L-cysteine
show the reaction diagram
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L-glutamate + dGTP + coenzyme F420-0 = dGDP + phosphate + coenzyme F420-1
show the reaction diagram
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deoxyGTP + UTP + NH4+ = deoxyGDP + phosphate + CTP
show the reaction diagram
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2'-dGTP + IMP + L-aspartate = 2'-dGDP + phosphate + adenylosuccinate
show the reaction diagram
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ATP + dGTP = P1-(5'-adenosyl),P4-[5'-(2'-deoxyguanosyl)]tetraphosphate + diphosphate
show the reaction diagram
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(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + dGTP = (ribonucleotide)20 + dGMP + diphosphate
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (17 results)

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EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
GTP + formate = dGTP + CO2 + H2O
show the reaction diagram
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dGDP + phosphoenolpyruvate = dGTP + pyruvate
show the reaction diagram
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dGDP + (phosphate)n+1 = dGTP + (phosphate)n
show the reaction diagram
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Enzyme Cofactor/Cosubstrate (8 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activation
-
can partly substitute for ATP
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Activator in Enzyme-catalyzed Reactions (152 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
highly activating, no effect on Km values
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heterotrophic allosteric activator
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very slight activation
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reactivation of ODC at pH values above 7.0
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at least 2fold enhancement of enzyme activity, maximal at 20 microM, similar results with all four deoxynucleoside triphosphates
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poor allosteric activator
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Inhibitor in Enzyme-catalyzed Reactions (126 results)

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EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
21% inhibition at 0.5 mM
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24% inhibition
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inhibits CDP reduction
more than 95% inhibition at 0.3 mM
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100 nM, 12% inhibition
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5 mM, 28°C, presence of MgCl2, 40% residual activity
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dGTP at a molar ratio of dGTP to dATP or rATP of 10:1 inhibits both DNA and RNA synthesis. Lower molar ratios of dGTP:rATP (0.1:1) inhibit ATP incorporation by 91%, whereas dATP incorporation is reduced by 8%
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purine triphosphate nucleotides (RTP) behave as non-competitive inhibitors, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs
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0.2 mM, 13% residual activity
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included along with 2 mM GTP in the incubation mixture. 28% inhibition
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competitive to ATP
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22% residual activity at 5 mM
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92% and 70% inhibition of RNA and DNA helicase activity at 2.5 mM, respectively
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inhibition of NTPase activity of NS3 protein by NTP derivatives
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92% and 70% inhibition of RNA and DNA helicase activity at 2.5 mM, respectively
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1 mM, inhibition is more pronounced at 37°C compared to 70°C
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no guanosine, kact: 1.5/sec, KA: 0.21 mM, Ki: 0.36 mM; the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
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slight
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3D Structure of Enzyme-Ligand-Complex (PDB) (1915 results)

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EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (65 results)

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EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.8
-
-
110
-
-
0.53
-
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
6.67
-
pH and temperature not specified in the publication
0.94
-
pH 8.0, 70°C
7.5
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pH 6.6, 25°C
1.5
-
no guanosine

KM Value (87 results)

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EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.017
-
-
0.117
-
-
0.041
-
pH 7.5
0.45
-
pH 7.2, 37°C
0.05
-
pH 8.0, 60°C, in presence of oligo(dC)
0.182
-
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
1.1
-
pH 8.0, 30°C
0.14
-
pH and temperature not specified in the publication
1
-
pH 8.0, 70°C
0.35
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pH 6.6, 25°C

Ki Value (22 results)

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EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
2
-
at pH 7.0, 30°C
0.4
-
-
3.5
-
pH 7.5, 25°C, inhibition of ATPase activity
0.277
-
inhibition of NTPase activity of NS3 protein by NTP derivatives
0.36
-
-

IC50 Value (5 results)

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EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.1
-
IC50: 0.1 mM
1.998
-
at pH 7.5 and 37°C
1.8
-
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction

References & Links

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Links to other databases for dGTP

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EXTERNAL LINKS
ChEBI
PubChem
UniChem