Ligand dGTP

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Basic Ligand Information

Molecular Structure
Picture of dGTP (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C10H16N5O13P3
dGTP
HAAZLUGHYHWQIW-KVQBGUIXSA-N
Synonyms:
2'-deoxy-GTP, 2'-deoxy-L-GTP, 2'-deoxyguanosine 5'-triphosphate, 2'-deoxyguanosine triphosphate, 2'-dGTP, 5'-dGTP, deoxyGTP, deoxyguanosine triphosphate


Show all pahtways known for Show all BRENDA pathways known for dGTP

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
dGTP + DNAn = diphosphate + DNAn+1
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-
dGTP + H2O = deoxyguanosine + triphosphate
show the reaction diagram
dGTP + H2O = dGDP + phosphate
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
dGDP + (phosphate)n+1 = dGTP + (phosphate)n
show the reaction diagram
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ATP + dGDP = ADP + dGTP
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (324 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
S-adenosyl-L-methionine + dGTP = S-adenosyl-L-homocysteine + m7dGTP
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dGTP + D-ribose = dGDP + D-ribose 5-phosphate
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dGTP + adenosylcobinamide = adenosylcobinamide phosphate + dGDP
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dGTP + adenosine = dGDP + AMP
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dGTP + thymidine = dGDP + dTMP
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dGTP + 2-deoxy-D-ribose = dGDP + 2-deoxy-D-ribose 5-phosphate
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dGTP + NAD+ = dGDP + NADP+
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pantothenate + dGTP = 4'-phosphopantothenate + dGDP
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dGTP + mevalonate = dGDP + phosphomevalonate
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dGTP + uridine = dGDP + UMP
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dGTP + 4-methyl-5-(2-hydroxyethyl)thiazole = dGDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
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dGTP + D-galactose = dGDP + alpha-D-galactose 1-phosphate
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dGTP + dAMP = dGDP + dADP
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dGTP + AMP = dGDP + ADP
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-
dGTP + dTMP = dGDP + dTDP
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dGTP + UMP = dGDP + UDP
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ATP + dGTP = diphosphate + ?
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dGTP + alpha-D-glucose 1-phosphate = dGDP-glucose + diphosphate
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dGTP + dGTP = P1,P4-bis(5'-(2'-deoxyguanosyl) tetraphosphate + diphosphate
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dGTP + pp(5')RNA = dG(5')ppp(5')RNA + diphosphate
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dGTP + RNAn = diphosphate + RNAn+1
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(2S)-2-phospholactate + dGTP = (2S)-lactyl-2-diphospho-5'-(2'-deoxy)guanosine + diphosphate
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p-tRNAHis + ATP + dGTP = ?
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dGTP + alpha-D-glucose 1-phosphate = diphosphate + dGDP-alpha-D-glucose
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dGTP + alpha-D-glucose 1-phosphate = diphosphate + dGDP-alpha-D-glucose
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dGTP + H2O = ?
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dGTP + H2O = deoxyguanosine + triphosphate
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dGTP + H2O = deoxyguanosine + triphosphate
show the reaction diagram
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dGTP + dihydrouracil + H2O = ?
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dGTP + H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-deoxyribosylamino)pyrimidine + diphosphate
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dGTP + H2O = 2-amino-5-formylamino-6-hydroxy-4-(deoxy-5-phosphoribosylamino)-pyrimidine + phosphate
show the reaction diagram
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dGTP + H2O = dGDP + phosphate
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dGTP + H2O = ?
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dGTP + H2O = dGMP + diphosphate
show the reaction diagram
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dGTP + H2O = dGMP + diphosphate
show the reaction diagram
-
dGTP + H2O = dGMP + diphosphate
show the reaction diagram
-
dGTP + H2O = dGMP + diphosphate
show the reaction diagram
-
dGTP + H2O = dGDP + phosphate
show the reaction diagram
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2'-deoxy-GTP = ?
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dGTP + H2O + closed Cl- channel = dGDP + phosphate + open Cl- channel
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dGTP + aminopterin + Glu = dGDP + phosphate + aminopteryl-Glu
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L-glutamate + dGTP + coenzyme F420-0 = dGDP + phosphate + coenzyme F420-1
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deoxyGTP + UTP + NH4+ = deoxyGDP + phosphate + CTP
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2'-dGTP + IMP + L-aspartate = 2'-dGDP + phosphate + adenylosuccinate
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ATP + dGTP = P1-(5'-adenosyl),P4-[5'-(2'-deoxyguanosyl)]tetraphosphate + diphosphate
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(ribonucleotide)10-3'-phosphate + 5'-hydroxy-(ribonucleotide)10 + dGTP = (ribonucleotide)20 + dGMP + diphosphate
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Product in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
GTP + formate = dGTP + CO2 + H2O
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-
GTP + reduced thioredoxin = dGTP + oxidized thioredoxin + H2O
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dGDP + phosphoenolpyruvate = dGTP + pyruvate
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dGDP + (phosphate)n+1 = dGTP + (phosphate)n
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Enzyme Cofactor/Cosubstrate (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activation
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Activator in Enzyme-catalyzed Reactions (140 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
highly activating, no effect on Km values
-
heterotrophic allosteric activator
-
very slight activation
-
reactivation of ODC at pH values above 7.0
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at least 2fold enhancement of enzyme activity, maximal at 20 microM, similar results with all four deoxynucleoside triphosphates
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poor allosteric activator
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Inhibitor in Enzyme-catalyzed Reactions (129 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
21% inhibition at 0.5 mM
-
24% inhibition
-
inhibits CDP reduction
more than 95% inhibition at 0.3 mM
-
100 nM, 12% inhibition
-
5 mM, 28°C, presence of MgCl2, 40% residual activity
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dGTP at a molar ratio of dGTP to dATP or rATP of 10:1 inhibits both DNA and RNA synthesis. Lower molar ratios of dGTP:rATP (0.1:1) inhibit ATP incorporation by 91%, whereas dATP incorporation is reduced by 8%
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purine triphosphate nucleotides (RTP) behave as non-competitive inhibitors, furthermore PARN does not discriminate whether there is ribose or deoxyribose in the nucleotides, Mg2+ releases the inhibition by RDPs and RTPs, but not by RMPs
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0.2 mM, 13% residual activity
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competitive inhibitor
included along with 2 mM GTP in the incubation mixture. 28% inhibition
-
competitive to ATP
-
inhibition of NTPase activity of NS3 protein by NTP derivatives
-
1 mM, inhibition is more pronounced at 37°C compared to 70°C
-
no guanosine, kact: 1.5/sec, KA: 0.21 mM, Ki: 0.36 mM; the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
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slight
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3D Structure of Enzyme-Ligand-Complex (PDB) (799 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (62 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.8
-
-
110
-
-
4000
-
-
0.53
-
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
6.67
-
pH and temperature not specified in the publication
0.94
-
pH 8.0, 70°C
7.5
-
pH 6.6, 25°C
1.5
-
no guanosine

KM Value (84 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.017
-
-
0.117
-
-
0.041
-
pH 7.5
0.45
-
pH 7.2, 37°C
0.05
-
pH 8.0, 60°C, in presence of oligo(dC)
0.182
-
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
1.1
-
pH 8.0, 30°C
0.14
-
pH and temperature not specified in the publication
1
-
pH 8.0, 70°C
0.35
-
pH 6.6, 25°C

Ki Value (22 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
2
-
at pH 7.0, 30°C
0.4
-
-
3.5
-
pH 7.5, 25°C, inhibition of ATPase activity
0.277
-
inhibition of NTPase activity of NS3 protein by NTP derivatives
0.36
-
-

IC50 Value (5 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.1
-
IC50: 0.1 mM
1.998
-
at pH 7.5 and 37°C
1.8
-
pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction

References & Links