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Ligand 3-(2'-methylthio)ethylmalate

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.2.1.170

2-(2-methylthio)ethylmalate = 2-(2-methylthio)ethylmaleate + H2O

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In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.3.17

2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalate + CoA

0

-

Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Substrate (2 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.85

3-(2'-methylthio)ethylmalate + NAD+ = ? + NADH + H+

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4.2.1.170

2-(2-methylthio)ethylmalate = 2-(2-methylthio)ethylmaleate + H2O

706255

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Product in Enzyme-catalyzed Reactions (4 results)

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Show Product (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.3.17

2-oxo-4-methylthiobutanoate + acetyl-CoA + H2O = 2-(2'-methylthio)ethylmalate + CoA

0

-

2.3.3.17

4-methylthio-2-oxobutanoate + acetyl-CoA + H2O = 2-(2'-methylthioethyl)-malate + CoA

0

-

2.3.3.17

4-methylthio-2-oxobutanoate + acetyl-CoA + H2O = 2-(2'-methylthioethyl)malate + CoA

0

-

2.3.3.17

4-methylthio-2-oxobutyrate + acetyl-CoA + H2O = 2-(2'-methylthioethyl)malate + CoA

0

-

2.3.3.17

4 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (6 results)

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Show Turnover Number (6 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.1.85

0.016

-

wild-type, pH not specified in the publication, temperature not specified in the publication

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1.1.1.85

0.0167

-

wild-type, pH not specified in the publication, temperature not specified in the publication

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1.1.1.85

0.033

-

mutant F137L, pH not specified in the publication, temperature not specified in the publication

725437

1.1.1.85

0.27

-

mutant L134F, pH not specified in the publication, temperature not specified in the publication

725437

1.1.1.85

0.367

-

mutant L133F, pH not specified in the publication, temperature not specified in the publication

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1.1.1.85

0.85

-

wild-type, pH not specified in the publication, temperature not specified in the publication

725437

1.1.1.85

6 entries

K_M Value (6 results)

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Show KM Value (6 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.1.85

0.045

-

wild-type, pH not specified in the publication, temperature not specified in the publication

725437

1.1.1.85

0.077

-

mutant L133F, pH not specified in the publication, temperature not specified in the publication

725437

1.1.1.85

0.103

-

mutant L134F, pH not specified in the publication, temperature not specified in the publication

725437

1.1.1.85

0.323

-

mutant F137L, pH not specified in the publication, temperature not specified in the publication

725437

1.1.1.85

0.436

-

wild-type, pH not specified in the publication, temperature not specified in the publication

725437

1.1.1.85

0.502

-

wild-type, pH not specified in the publication, temperature not specified in the publication

725437

1.1.1.85

6 entries

References & Links

Literature References (2)

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Show Reference (2 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

706255

19597944

Arabidopsis thaliana encodes a bacterial-type heterodimeric isopropylmalate isomerase involved in both Leu biosynthesis and the Met chain elongation pathway of glucosinolate formation

2009

Knill, T.; Reichelt, M.; Paetz, C.; Gershenzon, J.; Binder, S.

Plant Mol. Biol.

71

227-239

725437

21697089

Structural and functional evolution of isopropylmalate dehydrogenases in the leucine and glucosinolate pathways of Arabidopsis thaliana

2011

He, Y.; Galant, A.; Pang, Q.; Strul, J.; Balogun, S.; Jez, J.; Chen, S.

J. Biol. Chem.

286

28794-28801

Links to other databases for 3-(2'-methylthio)ethylmalate

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Show Links (1 entry)

ChEBI

PubChem