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Ligand [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH

Basic Ligand Information

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.8.1.15

ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor = AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor

737395, 738569, 738570, 739609

-

2.8.1.15

ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O = AMP + diphosphate + 5-methyl-2-thiouracil54 in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly

741346

-

2.8.1.15

2 entries

Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Substrate (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.8.1.15

ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyluridine54 in tRNA + acceptor = AMP + diphosphate + 5-methyl-2-thiouridine54 in tRNA + [TtuB sulfur-carrier protein] + reduced acceptor

737395, 738569, 738570, 739609

-

2.8.1.15

ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O = AMP + diphosphate + 2-thioribothymidine in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly

741379

-

2.8.1.15

ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH + 5-methyuracil54 in tRNA + H2O = AMP + diphosphate + 5-methyl-2-thiouracil54 in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly

741346

-

2.8.1.15

3 entries

Enzyme Kinetic Parameters

References & Links

Literature References (6)

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Show Reference (6 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

737395

27710943

Identification Crystallographic study of the 2-thioribothymidine-synthetic complex TtuA-TtuB from Thermus thermophilus

2016

Chen, M.; Narai, S.; Omura, N.; Shigi, N.; Chimnaronk, S.; Tanaka, Y.; Yao, M.

Acta Crystallogr. Sect. F

72

777.781

738569

16547008

Identification of two tRNA thiolation genes required for cell growth at extremely high temperatures

2006

Shigi, N.; Sakaguchi, Y.; Suzuki, T.; Watanabe, K.

J. Biol. Chem.

281

14296-306

738570

16317006

Identification Temperature-dependent biosynthesis of 2-thioribothymidine of Thermus thermophilus tRNA

2006

Shigi, N.; Suzuki, T.; Terade, T.; Shirouzu, M.; Yokoyama, S.; Watanabe, K.

J. Biol. Chem.

281

2104-2113

739609

23444054

Crystallographic and mutational studies on the tRNA thiouridine synthetase TtuA

2013

Nakagawa, H.; Kuratani, M.; Goto-Ito, S.; Ito, T.; Katsura, K.; Terada, T.; Shirouzu, M.; Sekine, S.; Shigi, N.; Yokoyama, S.

Proteins

81

1232-1244

741346

28439027

Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by an iron-sulfur protein TtuA

2017

Chen, M.; Asai, S.; Narai, S.; Nambu, S.; Omura, N.; Sakaguchi, Y.; Suzuki, T.; Ikeda-Saito, M.; Watanabe, K.; Yao, M.; Shigi, N.; Tanaka, Y.

Proc. Natl. Acad. Sci. USA

114

4954-4959

741379

23444054

Crystallographic and mutational studies on the tRNA thiouridine synthetase TtuA

2013

Nakagawa, H.; Kuratani, M.; Goto-Ito, S.; Ito, T.; Katsura, K.; Terada, T.; Shirouzu, M.; Sekine, S.; Shigi, N.; Yokoyama, S.

Proteins Struct. Funct. Bioinform.

81

1232-1244

Links to other databases for [TtuB sulfur-carrier protein]-Gly-NH-CH2-C(O)SH

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Show Links (1 entry)