Ligand N7-ribosyl-2,6-diamino-8-azapurine
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Basic Ligand Information
Molecular Structure

C9H13N7O4
N7-ribosyl-2,6-diamino-8-azapurine
ZBPCUFFVAWIZTM-UMMCILCDSA-N
N7-D-ribosyl-2,6-diamino-8-azapurine
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (1 result)
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N7-D-ribosyl-2,6-diamino-8-azapurine + phosphate = 2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
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Product in Enzyme-catalyzed Reactions (2 results)
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2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate = N7-D-ribosyl-2,6-diamino-8-azapurine + phosphate
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2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate = N8-ribosyl-2,6-diamino-8-azapurine + N7-ribosyl-2,6-diamino-8-azapurine + phosphate
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Enzyme Kinetic Parameters
KM Value (3 results)
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0.05
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about, pH 6.5, 25°C, recombinant mutant N243D
0.052
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pH 6.5, 25°C, recombinant wild-type enzyme
0.08
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about, pH 6.5, 25°C, recombinant wild-type enzyme
References & Links
Literature References (1)
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Site-selective ribosylation of fluorescent nucleobase analogs using purine-nucleoside phosphorylase as a catalyst effects of point mutations
2016
Stachelska-Wierzchowska, A.; Wierzchowski, J.; Bzowska, A.; Wielgus-Kutrowska, B.
Molecules
21
44
Links to other databases for N7-ribosyl-2,6-diamino-8-azapurine