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Enzyme Kinetic Parameters
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Ligand NMNH

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Basic Ligand Information

Molecular Structure

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Molecular Formula

BRENDA Name

InChIKey

C₁₁H₁₇N₂O₈P

NMNH

XQHMUSRSLNRVGA-TURQNECASA-N

Synonyms:

4-dihydronicotinamide mononucleotide, beta-NMNH, deamino-NMNH, nicotinamide mononucleotide-H2, nicotinamide phosphate, NMN(H), reduced nicotinamide mononucleotide

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Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (5 results)

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EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.6.1.13

NADH + H2O = AMP + NMNH

3.6.1.22

NADH + H2O = NMNH + AMP

209957, 209959

3.6.1.9

NADH + H2O = NMNH + AMP

246827, 208391, 246812, 246832, 246828, 246825, 246831, 0, 246801, 246834, 246833

3.6.1.9

NADPH + H2O = NMNH + adenosine 2',5'-bisphosphate

3.6.1.9

NADPH + H2O = NMNH + adenosine 2',5'-phosphate

246831, 246801

3.6.1.9

3 entries

Substrate in Enzyme-catalyzed Reactions (6 results)

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EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.2.3.1

clothianidin + NMNH = nitroso-clothianidin + amino-clothianidin + ?

673063

1.2.3.1

imidacloprid + NMNH = nitroso-imidacloprid + amino-imidacloprid + ?

673063

1.2.3.1

nitenpyram + NMNH = nitroso-nitenpyram + amino-nitenpyram + ?

NMNH + thio-NADP+ = NMN + thio-NADPH

392615

2.7.7.1

ATP + beta-NMNH = diphosphate + ?

642792

2.7.7.1

ATP + NMNH = diphosphate + NADH

672248

2.7.7.1

2 entries

Product in Enzyme-catalyzed Reactions (11 results)

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EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.7.7.50

NAD(H) + H2O = AMP + NMN(H)

3.6.1.13

NADH + H2O = AMP + NMNH

3.6.1.18

NADH + H2O = NMNH + AMP

3.6.1.22

deamino-NADH + H2O = deamino-NMNH + AMP

3.6.1.22

NADH + H2O = AMP + NMNH

209957, 209959, 0

3.6.1.22

NADH + H2O = NMNH + AMP

0, 209957, 209959

3.6.1.22

NADPH + H2O = NMNH + AMP

3.6.1.22

4 entries

3.6.1.45

NADH + H2O = NMNH + AMP

3.6.1.9

NADH + H2O = NMNH + AMP

246827, 208391, 246812, 246832, 0, 246828, 246825, 246831, 246801, 246834, 246833

3.6.1.9

NADPH + H2O = NMNH + adenosine 2',5'-bisphosphate

3.6.1.9

NADPH + H2O = NMNH + adenosine 2',5'-phosphate

246831, 0, 246801

3.6.1.9

3 entries

Inhibitor in Enzyme-catalyzed Reactions (4 results)

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EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

strong, kinetics

competitive inhibitor with Ki: 4.2 mM, no inhibition with NMN+

209957

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (3 results)

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EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.7.7.1

3.3

pH 7.5, 37°C, isozyme NMNAT2

672248

2.7.7.1

7.2

pH 7.5, 37°C, isozyme NMNAT3

672248

2.7.7.1

18.6

pH 7.5, 37°C, isozyme NMNAT1

672248

2.7.7.1

3 entries

K_M Value (10 results)

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EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.6.1.2

2.6

reduction of thio-NADP+ in energized submitochondrial particles

392615

1.6.1.2

3.4

reduction of thio-NADP+ in non energized submitochondrial particles

0.0304

0.13

NMNAT3

672248

2.7.7.1

0.13

pH 7.5, 37°C, isozyme NMNAT3

672248

2.7.7.1

0.294

NMNAT1

672248

2.7.7.1

0.294

pH 7.5, 37°C, isozyme NMNAT1

672248

2.7.7.1

0.304

NMNAT2

672248

2.7.7.1

0.304

pH 7.5, 37°C, isozyme NMNAT2

672248

2.7.7.1

0.625

37°C, pH 7.4

K_i Value (1 result)

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EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.6.1.22

4.1

competitive inhibitor, no inhibiton with NMN+

209957

References & Links

Literature References (20)

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Show Reference (20 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

208391

3001038

Purification and properties of nucleotide pyrophosphatase from human placenta

1985

Yano, T.; Funakoshi, I.; Yamashina, I.

J. Biochem.

1097-1107

209957

7829480

Cloning, purification, and properties of a novel NADH pyrophosphatase

1995

Frick, D.N.; Bessman, M.J.

J. Biol. Chem.

270

1529-1534

209959

10873676

Cloning and characterization of the NADH pyrophospatases from Caenorhabditis elegans and Saccharomyces cerevisiae, members of a nudix hydrolase subfamily

2000

Xu, W.; Dunn, C.A.; Bessman, M.J.

Biochem. Biophys. Res. Commun.

273

753-758

246801

10981813

Purification and properties of a nucleotide pyrophosphatase from lentil seddlings

2000

Medda, R.; Padiglia, A.; Lorrai, A.; Murgia, B.; Agro, A.F.; Castagnola, M.; Floris, G.

J. Protein Chem.

209-214

246812

2822037

Immunoaffinity purification and characterization of nucleotide pyrophosphatase from human placenta

1987

Yano, T.; Horie, K.; Kanamoto, R.; Kitagawa, H; Funakoshi, I.; Yamashina, I.

Biochem. Biophys. Res. Commun.

147

1061-1069

246825

1149736

Nucleotide pyrophosphatase of rat liver. A comparative study on the enzymes solubilized and purified from plasma membrane and endoplasmic reticulum

1975

Bischof, E.; Tran-Thi, T.A.; Decker, K.F.A.

Eur. J. Biochem.

353-361

246827

4360250

Purification and properties of a mouse liver plasma-membrane glycoprotein hydrolysing nucleotide pyrophosphate and phosphodiester bonds

1973

Evans, W.H.; Hood, D.; Gurd, J.W.

Biochem. J.

135

819-826

246828

4401058

Purification and properties of a yeast nucleotide pyrophosphatase

1972

Haroz, R.K.; Twu, J.S.; Bretthauer, R.K.

J. Biol. Chem.

247

1452-1457

246831

4403504

The purification and properties of detergent-solubilized rat liver nucleotide pyrophosphatase

1972

Bachorik, P.S.; Dietrich, L.S.

J. Biol. Chem.

247

5071-5078

246832

13549486

A "heat-activated" diphosphopyridine nucleotide pyrophosphatase from Proteus vulgaris

1958

Swartz, M.N.; Kaplan, N.O.; Lamborg, M.F.

J. Biol. Chem.

232

1051-1063

246833

13428775

A reduced pyridine nucleotide pyrophosphatase

1957

Jacobson, K.B.; Kaplan, N.O.

J. Biol. Chem.

226

427-437

246834

Nucleotide pyrophosphatase

1950

Kornberg, A.; Pricer, W.E.

J. Biol. Chem.

182

763-778

392615

4062305

Mitochondrial nicotinamide nucleotide transhydrogenase: nonidentical modification by N,N-dicyclohexylcarbodiimide and N-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline at the NAD(H) binding site

1985

Phelps, D.C.; Hatefi, Y.

Arch. Biochem. Biophys.

243

298-304

489739

4402158

Inhibition of nicotinamide phosphoribosyltransferase by pyridine nucleotides

1972

Dietrich, L.S.; Muniz, O.

Biochemistry

1691-1695

642779

1524439

NAD biosynthesis in human placenta: purification and characterization of homogeneous NMN adenylyltransferase

1992

Emanuelli, M.; Natalini, P.; Raffaelli, N.; Ruggieri, S.; Vita, A.; Magni, G.

Arch. Biochem. Biophys.

298

29-34

642792

7654174

NMN adenylyltransferase from bull testis: purification and properties

1995

Balducci, E.; Orsomando, G.; Polzonetti, V.; Vita, A.; Emanuelli, M.; Raffaelli, N.; Ruggieri, S.; Magni, G.; Natalini, P.

Biochem. J.

310

395-400

672248

17402747

Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis

2007

Sorci, L.; Cimadamore, F.; Scotti, S.; Petrelli, R.; Cappellacci, L.; Franchetti, P.; Orsomando, G.; Magni, G.

Biochemistry

4912-4922

673063

16411654

Substrate specificity of rabbit aldehyde oxidase for nitroguanidine and nitromethylene neonicotinoid insecticides

2006

Dick, R.A.; Kanne, D.B.; Casida, J.E.

Chem. Res. Toxicol.

38-43

703814

19273075

The NMN/NaMN adenylyltransferase (NMNAT) protein family

2009

Lau, C.; Niere, M.; Ziegler, M.

Front. Biosci.

410-431

741785

27769837

Ligand binding phenomena that pertain to the metabolic function of renalase

2016

Beaupre, B.A.; Roman, J.V.; Hoag, M.R.; Meneely, K.M.; Silvaggi, N.R.; Lamb, A.L.; Moran, G.R.

Arch. Biochem. Biophys.

612

46-56

Links to other databases for NMNH

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ChEBI

PubChem

Ligand NMNH

Basic Ligand Information

Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (5 results)

Substrate in Enzyme-catalyzed Reactions (6 results)

Product in Enzyme-catalyzed Reactions (11 results)

Inhibitor in Enzyme-catalyzed Reactions (4 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (3 results)

KM Value (10 results)

Ki Value (1 result)

References & Links

Literature References (20)

Links to other databases for NMNH

k_cat Value (Turnover Number) (3 results)

K_M Value (10 results)

K_i Value (1 result)