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Ligand 4-nitroaniline

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Basic Ligand Information

Molecular Structure
Picture of 4-nitroaniline (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
Molfile
C6H6N2O2
4-nitroaniline
TYMLOMAKGOJONV-UHFFFAOYSA-N
Synonyms:
4-aminonitrobenzene, 4-nitroanilide, 4-nitrolaniline, 4-nitronaniline, 4-nitrophenylamine, p-nitoaniline, p-nitroaniline, p-pitroaniline, para-nitroaniline, PNA

Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (9 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
L-Glu-4-nitroanilide + glutathione = 4-nitroaniline + 5-L-glutamyl-glutathione
show the reaction diagram
-
-
Glu-p-nitroanilide + H2O = glutamic acid + p-nitroaniline
show the reaction diagram
-
-
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O = Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
butyloxycarbonyl-L-Gln-L-Ala-L-Arg-4-nitroanilide + H2O = butyloxycarbonyl-L-Gln-L-Ala-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
D-Leu-Phg-Arg-4-nitroanilide + H2O = D-Leu-Phg-Arg + 4-nitroaniline
show the reaction diagram
-
-
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O = methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroanilide
show the reaction diagram
-
-
Z-Leu-Leu-Glu-NH-4-nitroanilide + H2O = Z-Leu-Leu-Glu + 4-nitroaniline
show the reaction diagram
-
-
4-nitroacetanilide + H2O = acetate + 4-nitroaniline
show the reaction diagram
-
-
4-nitrophenyl-3-ketovalidamine = 4-nitroaniline + 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxycyclohex-2-en-1-one
show the reaction diagram
-
-

Substrate in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
4-nitroaniline + NADH + H+ = ? + NAD+
show the reaction diagram
-
malonyl-CoA + 4-nitroaniline = CoA + N-(4-nitrophenyl)-malonamate
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (1959 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
5'-phospho-pyridoxyl-4-aminonitrobenzene + H2O + O2 = pyridoxal 5'-phosphate + 4-aminonitrobenzene + H2O2
show the reaction diagram
-
1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene + NAD(P)H = 4-nitrophenylamine + NAD(P)+ + 1-amino-2-hydroxy-6-sulfonaphthalene
show the reaction diagram
-
p-nitroacetanilide + aniline = p-nitroaniline + acetanilide
show the reaction diagram
-
gamma-glutamyl-p-nitroanilide + H2O = L-glutamate + p-nitroaniline
show the reaction diagram
-
L-gamma-glutamyl-p-nitroanilide + H2O = L-glutamine + p-nitroaniline
show the reaction diagram
-
-
L-gamma-glutamine-4-nitroanilide + H2O = L-glutamate + 4-nitroaniline
show the reaction diagram
-
-
Leu-4-nitroanilide + H2O = Leu + 4-nitroaniline
show the reaction diagram
-
L-leucyl-4-nitroanilide + H2O = L-leucine + 4-nitroaniline
show the reaction diagram
-
-
L-Asp-4-nitroanilide + H2O = L-Asp + 4-nitroaniline
show the reaction diagram
-
S-benzyl-L-Cys-4-nitroanilide + H2O = S-benzyl-L-Cys + 4-nitroaniline
show the reaction diagram
-
-
Gly-Pro-p-nitroanilide + H2O = Gly + Pro + p-nitroaniline
show the reaction diagram
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-
Suc-Ala-Ala-Pro-Phe-4-nitroanilide + H2O = Suc-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
-
-
Ac-Lys-Arg-4-nitroanilide + H2O = Ac-Lys-Arg + 4-nitroaniline
show the reaction diagram
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-
benzoyl-Arg 4-nitroanilide + H2O = benzoyl-Arg + 4-nitroaniline
show the reaction diagram
-
-
benzoyl-Ile-Glu-Gly-Arg 4-nitroanilide + H2O = benzoyl-Ile-Glu-Gly-Arg + 4-nitroaniline
show the reaction diagram
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-
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O = N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
-
-
Succinyl-tri-L-alanine 4-nitroanilide + H2O = Succinyl-tri-L-alanine + 4-nitroaniline
show the reaction diagram
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-
Ac-Arg-Val-Arg-Arg-4-nitroanilide + H2O = Ac-Arg-Val-Arg-Arg + 4-nitroaniline
show the reaction diagram
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-
succinyl-Ala-Ala-Pro-Phe 4-nitroanilide + H2O = succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
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-
peptide-4-nitroanilide + H2O = peptide + 4-nitroaniline
show the reaction diagram
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-
Glu-Asp-Val-Val-4-aminobutyryl-Cys-p-nitroanilide + H2O = Glu-Asp-Val-Val-4-aminobutyryl-Cys + 4-nitroaniline
show the reaction diagram
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N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O = N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
show the reaction diagram
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pyr-Glu-Phe-Leu-4-nitroanilide + H2O = pyr-Glu-Phe-Leu + 4-nitroaniline
show the reaction diagram
-
benzoyl-Asn-4-nitroanilide + H2O = benzoyl-Asn + 4-nitroaniline
show the reaction diagram
-
WEHD-4-nitroanilide + H2O = WEHD + 4-nitroaniline
show the reaction diagram
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-
acetyl-EFQLQ-4-nitroanilide + H2O = Ac-EFQLQ + 4-nitroaniline
show the reaction diagram
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-
N-benzyloxycarbonyl-FR-4-nitroanilide + H2O = N-benzyloxycarbonyl-FR + 4-nitroaniline
show the reaction diagram
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-
benzyloxycarbonyl-Arg-Arg-p-nitroanilide + H2O = benzyloxycarbonyl-Arg-Arg + p-nitroaniline
show the reaction diagram
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-
VLK-4-nitroanilide + H2O = VLK + 4-nitronaniline
show the reaction diagram
-
-
L-leucine-4-nitroanilide + H2O = L-leucine + 4-nitroaniline
show the reaction diagram
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-
D-Val-Leu-Lys-p-nitroanilide + H2O = D-Val-Leu-Lys + p-nitroaniline
show the reaction diagram
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-
Z-Ala-Ala-Leu-4-nitroanilide + H2O = Z-Ala-Ala-Leu + 4-nitroaniline
show the reaction diagram
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-
N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O = N-succinyl-Ala-Ala-Ala + 4-nitroaniline
show the reaction diagram
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-
N-alpha-benzoyl-arginine p-nitroanilide + H2O = N-alpha-benzoyl-Arg + 4-nitroaniline
show the reaction diagram
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-
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O = N-alpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
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succinyl-D-Asp-p-nitroanilide + H2O = succinyl-D-Asp + p-nitroaniline
show the reaction diagram
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-
phenylacetate 4-nitroanilide + H2O = phenylacetic acid + 4-nitrolaniline
show the reaction diagram
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-
N-(4-nitrophenyl)-6-aminohexanamide + H2O = 4-nitroaniline + 6-aminohexanoate
show the reaction diagram
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-
L-glutamate-p-nitroanilide + H2O = L-glutamate + 4-nitroaniline
show the reaction diagram
-
-
L-alanine-4-nitroanilide + H2O = L-alanine + 4-nitroaniline
show the reaction diagram
-
-
4-nitroacetanilide + H2O = 4-nitroaniline + acetate
show the reaction diagram
-
-
N-(4-nitrophenyl)-6-aminohexanamide + H2O = 4-nitroaniline + 6-aminohexanoate
show the reaction diagram
-
-
N-benzoyl-D-Arg-4-nitroanilide + H2O = N-benzoyl-D-Arg + 4-nitroaniline
show the reaction diagram
209232, -
-
ethyl N-nitrophenylcarbamate + H2O = 4-nitroaniline + CO2 + NH3
show the reaction diagram
-
-
L-gamma-glutamyl p-nitroanilide + H2O = L-glutamate + p-nitroaniline
show the reaction diagram
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-

Inhibitor in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
anti-parallel complementary PNA suppresses the degradation of double-stranded DNA by enzyme and the suppression is dependent on the amount and concentration of the PNA
-
inhibition at low concentration
-

3D Structure of Enzyme-Ligand-Complex (PDB) (78 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (7 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE

KM Value (1 result)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.28
0.37
-

Ki Value (1 result)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.25
-
-

References & Links

Links to other databases for 4-nitroaniline

EXTERNAL LINKS