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Ligand Na2S

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (4 results)

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Show Substrate (4 entries)

EC NUMBER

LITERATURE

REACTION DIAGRAM

REACTION

ENZYME 3D STRUCTURE

2.5.1.144

748059

azaserine + Na2S = ?

-

2.5.1.144

748059

O-acetyl-L-serine + Na2S = ?

-

2.5.1.144

2 entries

2.5.1.48

637454

O-succinyl-L-homoserine + sodium sulfide = L-homocysteine + sodium succinate

-

2.8.1.6

672142

dethiobiotin + Na2S = biotin

-

Product in Enzyme-catalyzed Reactions (1 result)

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Show Product (1 entry)

EC NUMBER

LITERATURE

REACTION DIAGRAM

REACTION

ENZYME 3D STRUCTURE

4.4.1.9

-

L-cysteine + NaCN = L-3-cyanoalanin + Na2S

-

Activator in Enzyme-catalyzed Reactions (1 result)

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Show Activating Compound (1 entry)

COMMENTARY

EC NUMBER

LITERATURE

ENZYME 3D STRUCTURE

activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.35fold

4.99.1.5

650189

-

Inhibitor in Enzyme-catalyzed Reactions (20 results)

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Show Inhibitors (20 entries)

COMMENTARY

EC NUMBER

LITERATURE

ENZYME 3D STRUCTURE

competitive vs. beta-hydroxybutyrate

1.1.1.30

286535

-

the enzyme activity is inhibited by up to 29-32% using 2 and 5 mM Na2S as H2S donor

1.1.1.40

762114

-

around 23% inhibition with 1 to 5 mM Na2S

1.1.1.42

762026

-

2 mM concentration 76% inhibition, 20 mM concentration 94% concentration

1.1.3.8

636324

-

-

1.17.1.9

288115

-

partially purified 6-HDH, 90% loss of activity

1.17.3.3

644533

-

-

1.4.1.9

349665

-

-

1.7.1.4

392934

-

-

2 entries

temporarily inhibition; together with sulfide no inhibition

1.7.2.4

394530

-

50% inhibition at 0.3 mM

1.7.2.6

394330

-

-

1.9.98.1

396332

-

50% inhibition at 1.3 mM

2.10.1.1

708986

-

-

2.5.1.49

637830

-

-

3.1.3.1

94613

-

-

3.11.1.2

22932

-

-

3 entries

60% loss of activity at 10 mM

3.4.11.10

81181

-

10 mM, 92% inhibition

3.4.11.14

647035

-

-

4.2.1.1

33531

-

-

4.6.1.19

24191

-

-

4.6.1.24

24191

-

-

3 entries

Metals and Ions (3 results)

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Show Metals Ions (3 entries)

COMMENTARY

EC NUMBER

LITERATURE

ENZYME 3D STRUCTURE

-

2.8.1.6

645587, 645595

-

enhances activity 3-4fold at 1 mM

2.8.1.6

645587

-

required for formation of mixed [Fe-S] cluster state

2.8.1.6

645613

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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Show Turnover Number (1 entry)

COMMENTARY

EC NUMBER

LITERATURE

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

30°C, pH 8.0

4.4.1.9

653464

1.5

-

K_M Value (6 results)

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Show KM Value (6 entries)

COMMENTARY

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

LITERATURE

-

2.5.1.48

0.55

-

637454

-

4.4.1.9

3.57

-

653511

-

4.4.1.9

8.24

-

653511

-

3 entries

30°C, pH 8.0

4.4.1.9

0.04

-

653464

pH 7.5

4.4.1.9

0.75

-

637388

pH 7.5

4.4.1.9

1.5

2

637388

pH 7.5

2 entries

K_i Value (1 result)

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Show KI Value (1 entry)

COMMENTARY

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

LITERATURE

-

1.7.1.4

5

-

392934

References & Links

Literature References (29)

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Show Reference (29 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

22932

8288524

In vitro characterization of a phosphate starvation-independent carbon-phosphorus bond cleavage activity in Pseudomonas fluorescens

1994

McMullan, G.; Quinn, J.P.

J. Bacteriol.

176

320-324

24191

-

Microbial ribonucleases with special reference to RNases T1, T2, N1, and U2

1971

Uchida, T.; Egami, F.

The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )

4

205-250

33531

3929690

Purification and some properties of carbonic anhydrase from bovine skeletal muscle

1985

Engberg, P.; Millqvist, E.; Pohl, G.; Lindskog, S.

Arch. Biochem. Biophys.

241

628-638

81181

4961737

Aeromonas aminopeptidase: purification and some general properties

1966

Prescott, J.M.; Wilkes, S.H.

Arch. Biochem. Biophys.

117

328-336

94613

413579

Properties of alkaline phosphatase of the halotolerant yeast Debaryomyces hansenii

1978

Adler, L.

Biochim. Biophys. Acta

522

113-121

286535

7213589

Inhibition of D(-)-beta-hydroxybutyrate dehydrogenase by modifiers of disulfides, thiols, and vicinal dithiols

1981

Phelps, D.C.; Hatefi, Y.

Biochemistry

20

453-458

288115

4301624

Formate dehydrogenase from the pea seedling

1968

Kanamori, T.; Suzuki, Y.

Enzymologia

35

185-197

349665

670223

Properties of crystalline leucine dehydrogenase from Bacillus sphaericus

1978

Ohshima, T.; Misono, H.; Soda, K.

J. Biol. Chem.

253

5719-5725

392934

4154942

Purification and properties of the Neurospora crassa assimilatory nitrite reductase

1974

Lafferty, M.A.; Garrett, R.H.

J. Biol. Chem.

249

7555-7567

394330

4154745

The partial characterization of purified nitrite reductase and hydroxylamine oxidase from Nitrosomonas europaea

1974

Ritchie, G.A.F.; Nicholas, D.J.D.

Biochem. J.

138

471-480

394530

-

Denitrification in Flexibacter canadensis

1990

Jones, A.M.; Knowles, R.

Can. J. Microbiol.

36

430-434

396332

4288725

Electron transport systems of the chemoautotroph Ferrobacillus ferrooxidans II. Purification and properties of a heat-labile iron-cytochrome c reductase

1966

Yates, M.G.; Nason, A.

J. Biol. Chem.

241

4872-4880

636324

5484453

Ascorbate synthesizing system in rat liver microsomes. I. Gulonolactone-reducible pigment as a prosthetic group of gulonolactone oxidase

1970

Nakagawa, H.; Assano, A.

J. Biochem.

68

737-746

637388

11575729

beta-Cyanoalanine synthase and cysteine synthase from potato: molecular cloning, biochemical characterization, and spatial and hormonal regulation

2001

Maruyama, A.; Saito, K.; Ishizawa, K.

Plant Mol. Biol.

46

749-760

637454

10595588

Cloning, purification, and characterization of cystathionine g-synthase from Nicotiana tabacum

1999

Clausen, T.; Wahl, M.C.; Messerschmidt, A.; Huber, R.; Fuhrmann, J.C.; Laber, B.; Streber, W.; Steegborn, C.

Biol. Chem.

380

1237-1242

637830

15989

O-Alkylhomoserine synthesis catalyzed by O-acetylhomoserine sulfhydrylase in microorganisms

1977

Murooka,Y.; Kakihara, K.; Miwa, T.; Seto, K.; Harada, T.

J. Bacteriol.

130

62-73

644533

-

Purification and characterization of the molybdoenzymes nicotinate dehydrogenase and 6-hydroxynicotinate dehydrogenase from Bacillus niacini

1990

Nagel, M.; Andreesen, J.R.

Arch. Microbiol.

154

605-613

645587

9862460

Biotin synthase mechanism: on the origin of sulfur

1998

Bui, B.T.S.; Florentin, D.; Fournier, F.; Ploux, O.; Mejean, A.; Marquet, A.

FEBS Lett.

440

226-230

645595

11444982

Spectroscopic changes during a single turnover of biotin synthase: Destruction of a [2Fe-2S] cluster accompanies sulfur insertion

2001

Ugulava, N.B.; Sacanell, C.J.; Jarrett, J.T.

Biochemistry

40

8352-8358

645613

11444981

Biotin synthase contains two distinct iron-sulfur cluster binding sites: Chemical and spectroelectrochemical analysis of iron-sulfur cluster interconversions

2001

Ugulava, N.B.; Gibney, B.R.; Jarrett, J.T.

Biochemistry

40

8343-8351

647035

4210360

Human liver aminopeptidase. Role of metal ions in mechanism of action

1974

Garner, C.W.; Behal, F.J.

Biochemistry

13

3227-3233

650189

14556637

A gene cluster responsible for alkylaldoxime metabolism coexisting with nitrile hydratase and amidase in Rhodococcus globerulus A-4

2003

Xie, S.X.; Kato, Y.; Komeda, H.; Yoshida, S.; Asano, Y.

Biochemistry

42

12056-12066

653464

10845460

Three Arabidopsis genes encoding proteins with differential activities for cysteine synthase and beta-cyanoalanine synthase

2000

Yamaguchi, Y.; Nakamura, T.; Kusano, T.; Sano, H.

Plant Cell Physiol.

41

465-476

653511

10889265

beta-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis

2000

Hatzfeld, Y.; Maruyama, A.; Schmidt, A.; Noji, M.; Ishizawa, K.; Saito, K.

Plant Physiol.

123

1163-1171

672142

16533066

Escherichia coli biotin synthase produces selenobiotin. Further evidence of the involvement of the [2Fe-2S]2+ cluster in the sulfur insertion step

2006

Tse Sum Bui, B.; Mattioli, T.A.; Florentin, D.; Bolbach, G.; Marquet, A.

Biochemistry

45

3824-3834

708986

15632135

In vitro molybdenum ligation to molybdopterin using purified components

2005

Nichols, J.D.; Rajagopalan, K.V.

J. Biol. Chem.

280

7817-7822

748059

6373737

Enzymatic proof for the identity of the S-sulfocysteine synthase and cysteine synthase B of Salmonella typhimurium

1984

Nakamura, T.; Iwahashi, H.; Eguchi, Y.

J. Bacteriol.

158

1122-1127

762026

30326260

Endogenous hydrogen sulfide (H2S) is up-regulated during sweet pepper (Capsicum annuum L.) fruit ripening. In vitro analysis shows that NADP-dependent isocitrate dehydrogenase (ICDH) activity is inhibited by H2S and NO

2018

Munoz-Vargas, M.A.; Gonzalez-Gordo, S.; Canas, A.; Lopez-Jaramillo, J.; Palma, J.M.; Corpas, F.J.

Nitric Oxide

81

36-45

762114

31152557

Inhibition of NADP-malic enzyme activity by H2S and NO in sweet pepper (Capsicum annuum L.) fruits

2020

Munoz-Vargas, M.A.; Gonzalez-Gordo, S.; Palma, J.M.; Corpas, F.J.

Physiol. Plant.

168

278-288

Links to other databases for Na2S

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Show Links (1 entry)

ChEBI

PubChem

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