Ligand ethanol

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Basic Ligand Information

Molecular Structure
Picture of ethanol (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C2H6O
ethanol
LFQSCWFLJHTTHZ-UHFFFAOYSA-N
Synonyms:
ethyl alcohol, EtOH


Show all pahtways known for Show all BRENDA pathways known for ethanol

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (22 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ethanol + NAD+ = acetaldehyde + NADH
show the reaction diagram
-
ethanol + NADP+ = acetaldehyde + NADPH + H+
show the reaction diagram
-
ethanol + NAD+ = ethanal + NADH
show the reaction diagram
-
ethanol + NAD(P)+ = acetaldehyde + NAD(P)H + H+
show the reaction diagram
-
ethanol + ferricytochrome c550 = acetaldehyde + ferrocytochrome c550
show the reaction diagram
-
ethanol + O2 = acetaldehyde + H2O2
show the reaction diagram
-
ethanol + N,N-dimethyl-4-nitrosoaniline = acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
show the reaction diagram
-
ethanol + acetyl-CoA = ethyl acetate + CoA
show the reaction diagram
-
UDP-glucuronate + ethanol = UDP + ethyl beta-D-glucuronoside
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (15 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
acetaldehyde + ubiquinol = ethanol + ubiquinone
show the reaction diagram
-
-
ethyl acetate + H2O = acetate + ethanol
show the reaction diagram
-
-
tabun + H2O = cyanophosphonic acid dimethylamide + ethanol
show the reaction diagram
-
-
2-oxopentanedioic acid 5-ethyl ester + H2O = 2-oxoglutarate + ethanol
show the reaction diagram
-

Substrate in Enzyme-catalyzed Reactions (144 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
ethanol + NAD+ = acetaldehyde + NADH + H+
show the reaction diagram
-
ethanol + NAD+ = acetaldehyde + NADH + H+
show the reaction diagram
-
ethanol + NAD+ = acetaldehyde + NADH
show the reaction diagram
-
ethanol + NAD+ = acetaldehyde + NADH
show the reaction diagram
-
ethanol + NAD+ + H2O = ethanal + NADH
show the reaction diagram
-
ethanol + NADP+ = acetaldehyde + NADPH + H+
show the reaction diagram
-
ethanol + NAD+ = acetaldehyde + NADH
show the reaction diagram
-
ethanol + NADP+ = ethanal + NADPH + H+
show the reaction diagram
-
ethanol + NAD+ = acetaldehyde + NADH
show the reaction diagram
-
ethanol + NADP+ = acetaldehyde + NADPH + H+
show the reaction diagram
-
ethanol + NAD+ = ethanal + NADH
show the reaction diagram
-
ethanol + ferricytochrome c = acetaldehyde + ferrocytochrome c + H+
show the reaction diagram
-
ethanol + N,N,N',N'-tetramethyl-4-phenylenediamine = acetaldehyde + reduced N,N,N',N'-tetramethyl-4-phenylenediamine
show the reaction diagram
-
ethanol + O2 = ethanal + H2O2
show the reaction diagram
-
ethanol + O2 = acetaldehyde + H2O2
show the reaction diagram
-
ethanol + a quinone = acetaldehyde + a quinol
show the reaction diagram
-
ethanol + phenazine methosulfate = acetaldehyde + reduced phenazine methosulfate
show the reaction diagram
-
ethanol + acceptor = ethanal + reduced acceptor
show the reaction diagram
-
ethanol + acceptor = acetaldehyde + reduced acceptor
show the reaction diagram
-
ethanol + N,N-dimethyl-4-nitrosoaniline = acetaldehyde + 4-(hydroxylamino)-N,N-dimethylaniline
show the reaction diagram
-
ethanol + H2O2 = acetaldehyde + ?
show the reaction diagram
-
ethanol + O2 = ?
show the reaction diagram
-
ethanol + p-nitroso-N,N-dimethylaniline = ethanal + reduced p-nitroso-N,N-dimethylaniline
show the reaction diagram
-
ethanol + acetyl-CoA = ethyl acetate + CoA
show the reaction diagram
-
sucrose + ethanol = D-glucose + 2-O-ethyl-beta-D-fructofuranose
show the reaction diagram
-
UDP-glucuronate + ethanol = UDP + ethyl beta-D-glucuronoside
show the reaction diagram
-
O-acetyl-L-homoserine + ethanol = O-ethyl-homoserine + acetic acid
show the reaction diagram
-
ATP + ethanol = ADP + ethyl phosphate
show the reaction diagram
-
3'-phosphoadenylylsulfate + ethanol = adenosine 3',5'-bisphosphate + ethyl sulfate
show the reaction diagram
-
gallate + ethanol = ethyl gallate + H2O
show the reaction diagram
-
ethanol + caproate = ethyl caproate + H2O
show the reaction diagram
-
p-nitrophenyl phosphate + ethanol = p-nitrophenyl phosphate + ethylphosphate
show the reaction diagram
-
D-glucose + ethanol = ethyl-alpha-D-glucoside
show the reaction diagram
-
ethanol + phenyl alpha-D-galactoside = ?
show the reaction diagram
-
4-O-beta-D-mannopyranosyl-D-mannopyranose + ethanol = ?
show the reaction diagram
-
N-acetyllactosamine + ethanol = ethyl beta-N-acetyllactosaminide + H2O
show the reaction diagram
dextran + ethanol = ethyl alpha-isomaltoside + H2O
show the reaction diagram
-
beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-p-nitrophenyl + ethanol = beta-D-Gal-(1-3)-alpha-D-GalNAc-(1-O)-ethyl + p-nitrophenol
show the reaction diagram
-
N-benzoyl-L-tyrosine methyl ester + ethanol = N-benzoyl-L-tyrosine ethyl ester + methanol
show the reaction diagram
-
L-glyceraldehyde 3-phosphate + ethanol = 2-deoxy-5-O-phosphono-L-threo-pentose
show the reaction diagram
-
ATP + H2O + ethanol/in = ADP + phosphate + ethanol/out
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (343 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
-
acetaldehyde + NADH + H+ = ethanol + NAD+
show the reaction diagram
-
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
acetaldehyde + NADH + H+ = ethanol + NAD+
show the reaction diagram
-
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
-
acetaldehyde + NADH + H+ = ethanol + NAD+
show the reaction diagram
-
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
-
acetaldehyde + NADH + H+ = ethanol + NAD+
show the reaction diagram
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
-
acetaldehyde + NADPH + H+ = ethanol + NADP+
show the reaction diagram
-
-
acetaldehyde + NAD+ = ethanol + NADH + H+
show the reaction diagram
-
-
acetaldehyde + 2 reduced cytochrome cL = ethanol + 2 oxidizeded cytochrome cL
show the reaction diagram
-
-
ethane + NADH + H+ + O2 = ethanol + NAD+ + H2O
show the reaction diagram
-
-
7-ethoxyresorufin + [reduced NADPH-hemoprotein reductase] + O2 = resorufin + ethanol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
glutamic acid gamma methyl ester + ethylamine = theanine + ethanol
show the reaction diagram
-
-
2-mercaptoethanol + cyanide = ethanol + thiocyanate
show the reaction diagram
-
-
ethyl oleate + H2O = oleic acid + ethanol
show the reaction diagram
-
-
ethyl acetate + H2O = ethanol + acetate
show the reaction diagram
-
-
ethyl palmitate + H2O = ethanol + palmitate
show the reaction diagram
-
-
ethyl acetate + H2O = ethanol + acetate
show the reaction diagram
-
-
diethylphthalate + H2O = ethylphthalate + ethanol
show the reaction diagram
-
-
ethyl n-butyrate + H2O = ethanol + n-butanoate
show the reaction diagram
-
-
pimeloyl-[acyl-carrier protein] ethyl ester + H2O = pimeloyl-[acyl-carrier protein] + ethanol
show the reaction diagram
-
-
adenosine 5'-monophosphate-O-ethyl ester + H2O = adenosine 5'-monophosphate + ethanol
show the reaction diagram
-
ethyl phosphate + H2O = ethanol + phosphate
show the reaction diagram
-
-
Ethyl-phosphate + H2O = Ethanol + phosphate
show the reaction diagram
-
-
ethyl alpha-D-galactoside + H2O = ethanol + D-galactose
show the reaction diagram
-
-
ethyl-beta-D-galactoside + H2O = ethanol + beta-D-galactose
show the reaction diagram
-
-
ethyl-beta-N-acetyl-glucosaminide + H2O = ethanol + beta-N-acetyl-glucosamine
show the reaction diagram
-
-
L-leucine ethyl ester + H2O = L-leucine + ethanol
show the reaction diagram
-
N-acetyl-Ala ethyl ester + H2O = N-acetyl-Ala + ethanol
show the reaction diagram
-
ubiquitin ethyl ester + H2O = ubiquitin + ethanol
show the reaction diagram
-
-
ethyl L-pyroglutamate + H2O = L-pyroglutamate + ethanol
show the reaction diagram
-
-
N-acetyl-L-tyrosine ethyl ester + H2O = N-acetyl-L-tyrosine + ethanol
show the reaction diagram
-
-
benzoyl-Tyr-ethyl ester + H2O = benzoyl-Tyr + ethanol
show the reaction diagram
-
-
Nalpha-Benzoyl-L-Arg ethyl ester + H2O = Nalpha-Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
benzoyl arginine ethyl ester + H2O = benzoyl-Arg + ethanol
show the reaction diagram
-
-
N-acetyl-Tyr ethyl ester + H2O = N-acetyl-Tyr + ethanol
show the reaction diagram
-
-
benzoyl-Arg ethyl ester + H2O = benzoyl-Arg + ethanol
show the reaction diagram
-
-
benzoyl-Arg ethyl ester + H2O = benzoyl-Arg + ethanol
show the reaction diagram
-
-
Nalpha-Benzoyl-L-Arg ethyl ester + H2O = Nalpha-Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
N-Benzoyl-L-Arg ethyl ester + H2O = N-Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
benzoyl-Arg ethyl ester + H2O = benzoyl-Arg + ethanol
show the reaction diagram
-
-
Benzoyl-L-Arg ethyl ester + H2O = Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
benzoyl-arginine ethyl ester + H2O = benzoyl-arginine + ethanol
show the reaction diagram
-
N-benzoyl-L-arginine ethyl ester + H2O = N-benzoyl-L-arginine + ethanol
show the reaction diagram
-
N-Benzoyl-L-Arg ethyl ester + H2O = N-Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
N-benzoyl-L-Arg ethyl ester + H2O = N-benzoyl-Arg + ethanol
show the reaction diagram
-
-
N-Benzoyl-L-Arg ethyl ester + H2O = N-Benzoyl-L-Arg + ethanol
show the reaction diagram
-
-
N-benzoyl-L-tyrosine ethyl ester + H2O = N-benzoyl-Tyr + ethanol
show the reaction diagram
-
-
N-acetyl-L-tyrosine ethyl ester + H2O = N-acetyl-L-tyrosine + ethanol
show the reaction diagram
-
-
diethyl phenylmalonate + H2O = (+)-ethyl phenylmalonate + ethanol
show the reaction diagram
-
-
2-oxopentanedioic acid 5-ethyl ester + H2O = 2-oxoglutarate + ethanol
show the reaction diagram
-
2-hydroxyethylflavin + H2O = ethanol + lumichrome
show the reaction diagram
-
-
ATP + H2O + ethanol/in = ADP + phosphate + ethanol/out
show the reaction diagram
-
-

Activator in Enzyme-catalyzed Reactions (146 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
activation, used as nonfermentable carbon source in wild type and tpa1 mutant cell cultures, greater activity compared to activity in glucose-grown cells
-
does not affect the adhS gene expression but induces PQQ-ADH activity
-
100 mM, 1.5fold increase in activity
-
ethanol does not affect the adhS gene expression but induces PQQ-ADH activity
-
activation
-
stimulation
-
10% v/v, 2fold activation
-
15%, 1.7fold activation. Strong decrease in activity above 15%
-
activity is enhanced in the presence of 10-50% (v/v)
-
presence of ethanol enhances the hydroxylation by resting non-induced cells
-
increases maximal reaction rate
-
30% v/v, 5fold activation
-
0.5-2% increases activity in crude extracts
-
0.05 mM, 1.8fold stimulation
-
a 3fold increase in the protein content of the E1o component 2-oxoglutarate dehydrogenase complex is observed in liver mitochondria of rats exposed to ethanol
-
1 M, increases activity by about 10%
-
activates up to 40% v/v
-
2fold activation in the presence of ethanol
-
activation, 12% v/v
-
enhances the inducing and protecting effects of betaine on the enzyme, alone has only a slightly activating effect on the enzyme activity, overview
-
activation, 1-10% v/v
-
increases activity
-
addition of 14% leads to 2fold increase of enzyme activity
-
highest activity when diacylglycerol is in ethanol
-
integral membrane enzyme, 2% (v/v), 30% increase in activity
-
5fold activation at 10% v/v
-
enzyme activity is slightly elevated at a lower concentration of ethanol at 25C. With increasing ethanol concentration, the enzyme activity decreases, and in the presence of 50% ethanol the enzyme activity is almost lost after incubation for 30 min at 25C
-
strong activation of enzyme in hepatocytes
-
ethanol increases the fructooligosaccharide biosynthesis activity of levansucrase, fructooligosaccharide production at different temperatures in the presence of ethanol, inverse relationship between temperature and ratio synthetic activity/total activity of levansucrase, overview
-
increase of activity at 7-20%
-
stress treatment induces higher levels of OGT protein expression
-
binge ethanol treatment causes significant increase in blood plasma AST activity
-
activates
-
little activity unless activated either by a complex of 70S ribosomes, mRNA and uncharged tRNA or by a solvent like ethanol at approximately 20%
-
activates 1.5fold at 5% v/v
-
ethanol increases the enzyme activity to 136%
-
activates even at higher concentrations
-
10% (v/v): 100% activity compared to no addition
-
activation
80% ethanol, activation of both isoenzymes, conversion of isoenzyme H-1 to a 35500 Da form and isoenzyme H-2 to a 67000 Da form and a 35500 Da form
-
livers from wild type mice treated with ethanol (2 g/kg of 20% ethanol) release 2-3fold higher levels of adenosine than livers from comparably treated CD73KO mice
-
15% increase of activity at 50% (v/v) ethanol, at 30C
-
1% v/v ethanol: slight activation
-
64% stimulation at a concentration of 20%, hydrolysis of cholesteryl beta-D-glucoside. 65% stimulation at a concentration of 20%, hydrolysis of p-nitrophenyl beta-D-glucoside
-
enzyme activity increases to 113% in the presence of 10% (v/v) ethanol
-
about 127% activity at 25% (v/v)
-
13% activation at 10% w/v
-
transglycosylation, activation
-
the liberation of aglycone from aryl beta-D-glucosides is stimulated by alcohols in a manner suggesting specific interaction between alcohol and enzyme
-
alcohol modifies the protein microenvironment, leading to a more flexible enzyme structure, which is probably responsible for the enhanced enzymatic activity
-
2.7 M, stimulates
-
116% activity in the presence of 1 mM
-
25% v/v, 6fold increase of the activity, methoxycarbonyl-D-Nle-Gly-Arg-p-nitroanilide as substrate, rFIXa, alcohols modify the conformation of FIXa rendering the active-site cleft more easily accessible to tripeptide substrates with a hydrophobic residue in the P3-position
-
in 10% (v/v) ethanol, the enzyme significantly increases its activity by 50%
-
activation e.g. between 2.5-12%, v/v, depending on substrate
-
slight activation at concentration of 4 M
-
stimulation by aliphatic alcohols in a decreasing order of effectiveness: ethylene glycol > 2-propanol > ethanol > glycerol > methanol > 1-propanol
-
activates
-
10% w/v, increases activity by 24%
-
all PLC1-6 are activated in salt/ethanol extractions
-
1.6fold increase of activity when added to the enzyme test system at a concentration of 6%
-
stimulates
-
slight stimulation at low tRNA concentrations
-
feeding in vivo increases the enzyme expression, treatment of hepatocytes induces the expression of only the heavy enzyme subunit
-

Inhibitor in Enzyme-catalyzed Reactions (279 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
10 mM, more than 90% inhibition
-
40% (v/v), 30% activity
-
20% (v/v), 20% loss of activity
-
3.7% residual activity at 10% (v/v)
-
19% residual activity at 50% (v/v)
-
10% v/v: 25-75% loss of activity, depending on substrate, enzyme regains activity after removal of alcohol
-
3.6% residual activity at 80% (v/v)
-
16% inhibition at 2 M, 48% inhibition at 4.7 M
-
substrate inhibition
-
the native enzyme shows less than 5% residual activity in the presence of ethanol
-
5% v/v, 50% inhibition, complete loss of activity at 20% v/v
-
5%, 10% inhibition
-
45% inhibition at 10% concentration as co-solvent
-
10%, v/v, complete inhibition of hydroxylation
-
50% ethanol, 56% inhibition
-
depression
-
complete inhibition at 40% (v/v)
-
IC50 of 4.8 vol%
-
competitive inhibition
-
substrate n-butanol
-
43% inhibition when 0.04 ml are added to reaction mixture
-
integral membrane enzyme, 10%, 20% inhibition
-
irreversible at 2% v/v
-
precipitation with ethanol completely inhibits enzyme activity
-
at 4% inhibits
-
substrate inhibition above 0.5 M
-
complete inhibition at 1.6 M
-
traces inactivate the enzyme
-
10% (v/v), 28% inhibition; 72% inhibition
-
50% inhibition of isozyme UGT2B17 at 1%
-
about 57.2% decreased enzyme activity, 10%
-
15%, about 3 M, about 50% inhibition of product formation
-
20% v/v, 4% inhibition
-
5 mM, 47% loss of activity
-
20% v/v, 42% inhibition
-
inhibits the mu- and pi-class GST activities in vivo by 53% and 13%, respectively, in hepatocytes and the culture medium, the enzyme activity is increased in the cell medium, overview, the inhibitory effect is completely oe partially reversible by vitamin E or 4-methylpyrazole, respectively
-
25 mM ethanol substantially decreases the enzymatic activity of MAT II
-
0.5%, 20% inhibition
-
dose-dependent inhibition of insulin receptor substrate-1-associated PI3K activity in skeletal muscle, but not in adipose tissue, ethanol-induced diabetic rats
-
10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v)
-
0.03%, inhibition of PKCgamma oscillation at 0.1 mM
-
decreases phosphorylation of eEF2K, whereas elongation factor 2 phosphorylation increases, but this response is not mediated by eEF2K
-
concentrations above 0.005 ml/0.2 ml assay
-
about 40% residual activity at 30% (v/v)
-
10 mM, 20% inhibition
-
6%, 85% inhibition
-
inhibits the enzyme, LPL activity is reduced by 23-25% 2-4 hours after the administration of alcohol, respectively, with respect to controls, in vivo or in post-heparin plasma. The concentrations of inhibitors ANGPTL3 and ANGPTL4 drops to 67% and 86% of baseline values, respectively, at 280 min after alcohol consumption. The acute effects of alcohol consumption, especially on postprandial lipemia, seem to be accentuated in hypertriglyceridemic subjects
-
product inhibition
-
97% inhibition at 75% v/v
-
80-90% inhibition
-
reversible noncompetitive
-
inhibition of PAP-1
-
23% and 28% inhibition at 0.5% and 1% (v/v) ethanol, respectively
-
at a concentration above 2%
-
astrocyte treatment with ethanol inhibits the activity of arylsulfatase B, and triggers the degradation of chondroitin-4-sulfate, increases total sulfated glycosaminoglycans, chondroitin-4-sulfate and neurocan core-protein content and inhibits neurite outgrowth in neurons cocultured with ethanol-treated astrocytes in vitro. Ethanol also inhibits arylsulfatase B activity and increases sulfated glycosaminoglycans and neurocan levels in the developing hippocampus after in vivo ethanol exposure
-
decreases ASA activity levels in the liver, but not in the cerebral cortex and kidney
-
10% ethanol: inactivation
-
39% inhibition at a concentration of 50%, hydrolysis of p-nitrophenyl beta-D-glucoside
-
20% (v/v), 2 h, 20C, 39% loss of activity
-
excess amounts inhibit
-
20%, 91% inhibition
-
41% residual activity at 1 mM
-
30% inhibition at 10% and 75% at 25%
-
moderate inhibition at 5 mM
-
10% v/v, about 40% loss of activity
-
5 mM, no residual activity
-
83.3% residual activity at 10% (v/v)
-
inhibition above 50 mM
-
complete inhibition at 10%
-
inhibition reversible when up to 80% ethanol concentration used
-
5%, 16% inhibition of activity with carboxymethyl cellulose, 24% inhibition of xylanase activity, fusion enzyme (EG-M-Xyn) of endoglucanase (cellulase) from Teleogryllus emma and xylanase from Thermomyces lanuginosus
20%, 29.5% loss of activity
-
10% (v/v), about 50% inhibition
-
completely inactivated by ethanolH2O of above 1:2 (v/v)
-
Ki: 80 mM
-
inactivation above 30%
-
strong inhibitor
-
10%, complete inhibition
-
the enzyme loses about half of its activity at 5% (v/v) ethanol
-
75.09% residual activity at 50% (v/v)
-
80-40% loss of activity, thiobenzyl benzyloxycarbonyl-L-lysinate as substrate
-
activity decreases with increasing ethanol content, up to 15% ethanol papain from papaya latex is less sensitive to ethanol
-
about 90% residual activity at 2 mM
-
ethanol-induced proteasome inhibition in liver cells
-
slight inhibition
-
50% inhibition at 30% v/v
-
50% inhibition at 30% v/v
-
CD spectra of adenosine deaminase and EtOH, determination of helix content, MD calculations
-
a relative activity of 49% is detected in 5% (v/v) of ethanol
-
competitive
-
12%, slight inhibition of activity in cell suspension, not: up to 10%
-
high concentrations
-
at 12.5% (v/v) remaining activity, 79.19%, and 25% (v/v) remaining activity, 33.41%
-
15-20% of maximum activity when assayed in 40% (v/v) ethanol
-
less than 60% residual activity at 6% (v/v) ethanol
-
2% (v/v), 68.6% inhibition
-
5% ethanol causes strongly reduced activity
-
moderate, nonspecific
-
maximal activation, 10fold, at 15% v/v. Inactivation at 20% v/v
-

Metals and Ions (2 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
15% v/v, 1.7fold activation. 30% v/v, 18% loss of activity
-
activates by 54% at 5% v/v
-

3D Structure of Enzyme-Ligand-Complex (PDB) (126 results)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (80 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
7.1
-
pH 7.5, 37C, recombinant enzyme
58
-
pH 7.5, 30C
123
-
pH 7.5, 70C, wild-type enzyme
7.5
-
pH 7.5, 30C

KM Value (255 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.2225
-
at pH 8.8 and 37C
0.94
-
cosubstrate: NAD+
343
-
-
46
-
pH 7.5, 37C, recombinant enzyme
343
-
-
35
-
pH and temperature not specified in the publication
14
-
pH 8.5, 23C
0.26
-
-
6.5
-
pH 8.5, 30C
3.39
-
pH 9.0, 30C, assayed with N,N,N',N'-tetramethyl-4-phenylenediamine
0.00018
-
pH 7.5, 70C, wild-type enzyme
0.082
-
pH 7.0, 45C
0.02
-
+ p-nitroso-N,N-dimethylaniline
7.2
-
microsomal ethanol oxidizing system
3.12
-
at pH 7.5 and 30C
4.35
-
pH 7.5, 30C
2100
-
-
40
-
-
202
-
-

Ki Value (10 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
TABLE ID
33
-
pH 7.0, 45C
1951
147
-
-
6322
35
-
-
6773
90
-
substrate n-butanol
2274
1900
-
substrate 4-nitrophenyl-beta-D-xylopyranoside, 25C, pH 7.0
20418
80
-
-
22150
100
-
pH 7.5, 25C
32056

IC50 Value (3 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
-999
-
IC50: 15% (v/v)

References & Links