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Ligand (2S)-2-hydroxy-3-oxopropane-1-sulfonic acid

Basic Ligand Information

Molecular Structure

Related pathways

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Natural Substrate (3 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.373

(S)-3-sulfolactaldehyde + NADH + H+ = (S)-2,3-dihydroxypropane-1-sulfonate + NAD+

762529

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1.2.1.97

(2S)-3-sulfolactaldehyde + NAD+ + H2O = (2S)-3-sulfolactate + NADH + H+

735194, 763084

-

1.2.1.97

(2S)-3-sulfolactaldehyde + NADP+ + H2O = (2S)-3-sulfolactate + NADPH + H+

735194

-

1.2.1.97

2 entries

In Vivo Product in Enzyme-catalyzed Reactions (2 results)

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Show Natural Product (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.1.2.57

6-deoxy-6-sulfo-D-fructose 1-phosphate = glycerone phosphate + (2S)-3-sulfolactaldehyde

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-

4.1.2.58

2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate = (2S)-3-sulfolactaldehyde + pyruvate

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-

Substrate in Enzyme-catalyzed Reactions (4 results)

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Show Substrate (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.1.1.373

(S)-3-sulfolactaldehyde + NADH + H+ = (S)-2,3-dihydroxypropane-1-sulfonate + NAD+

762529

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1.2.1.97

(2S)-3-sulfolactaldehyde + NAD+ + H2O = (2S)-3-sulfolactate + NADH + H+

735194, 763084

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1.2.1.97

(2S)-3-sulfolactaldehyde + NADP+ + H2O = (2S)-3-sulfolactate + NADPH + H+

735194

-

1.2.1.97

2 entries

4.1.2.57

glycerone phosphate + (2S)-3-sulfolactaldehyde = 6-deoxy-6-sulfo-D-fructose 1-phosphate

768188

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Product in Enzyme-catalyzed Reactions (4 results)

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Show Product (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.2.1.14

6-deoxy-6-sulfo-D-fructose + D-erythrose 4-phosphate = (2S)-3-sulfolactaldehyde + D-sedoheptulose 7-phosphate

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2.2.1.14

6-deoxy-6-sulfo-D-fructose + D-glyceraldehyde 3-phosphate = (2S)-3-sulfolactaldehyde + beta-D-fructofuranose 6-phosphate

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-

2.2.1.14

2 entries

4.1.2.57

6-deoxy-6-sulfo-D-fructose 1-phosphate = glycerone phosphate + (2S)-3-sulfolactaldehyde

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-

4.1.2.58

2-dehydro-3,6-dideoxy-6-sulfo-D-gluconate = (2S)-3-sulfolactaldehyde + pyruvate

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-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (6 results)

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Show Turnover Number (6 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.1.1.373

27

-

mutant enzyme T124G, at pH 8.0 and 30°C

762529

1.1.1.373

68

-

mutant enzyme R123G, at pH 8.0 and 30°C

762529

1.1.1.373

71

-

mutant enzyme G122S, at pH 8.0 and 30°C

762529

1.1.1.373

332

-

wild type enzyme, at pH 8.0 and 30°C

762529

1.2.1.97

11.4

-

with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication

735194

1.2.1.97

11.8

-

with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication

735194

1.1.1.373

4 entries

1.2.1.97

2 entries

K_M Value (6 results)

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Show KM Value (6 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.1.1.373

0.3

-

wild type enzyme, at pH 8.0 and 30°C

762529

1.1.1.373

1.63

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mutant enzyme G122S, at pH 8.0 and 30°C

762529

1.1.1.373

5.74

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mutant enzyme T124G, at pH 8.0 and 30°C

762529

1.1.1.373

7.99

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mutant enzyme R123G, at pH 8.0 and 30°C

762529

1.2.1.97

0.14

-

with NAD+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication

735194

1.2.1.97

0.14

-

with NADP+ as cosubstrate, in 50 mM Tris-HCl (pH 7.5), temperature not specified in the publication

735194

1.1.1.373

4 entries

1.2.1.97

2 entries

References & Links

Literature References (5)

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Show Reference (5 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

735194

26195800

Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1

2015

Felux A.-K, F.A.; Spiteller D, S.D.; Klebensberger J, K.J.; Schleheck D, S.D.

Proc. Natl. Acad. Sci. USA

112

E4298-E4305

735194

26195800

Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1

2015

Felux, A.K.; Spiteller, D.; Klebensberger, J.; Schleheck, D.

Proc. Natl. Acad. Sci. USA

112

E4298-E4305

735194

2 entries

762529

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Dynamic structural changes accompany the production of dihydroxypropanesulfonate by sulfolactaldehyde reductase

2020

Sharma, M.; Abayakoon, P.; Lingford, J.; Epa, R.; John, A.; Jin, Y.; Goddard-Borger, E.; Davies, G.; Williams, S.

ACS Catal.

10

2826-2836

763084

30546350

Anaerobic degradation of the plant sugar sulfoquinovose concomitant with H2S production Escherichia coli K-12 and Desulfovibrio sp. strain DF1 as co-culture model

2018

Burrichter, A.; Denger, K.; Franchini, P.; Huhn, T.; Müller, N.; Spiteller, D.; Schleheck, D.

Front. Microbiol.

9

2792

768188

33791429

Molecular basis of sulfosugar selectivity in sulfoglycolysis

2021

Sharma, M.; Abayakoon, P.; Epa, R.; Jin, Y.; Lingford, J.P.; Shimada, T.; Nakano, M.; Mui, J.W.; Ishihama, A.; Goddard-Borger, E.D.; Davies, G.J.; Williams, S.J.

ACS Cent. Sci.

7

476-487

Links to other databases for (2S)-2-hydroxy-3-oxopropane-1-sulfonic acid

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Show Links (1 entry)