print visible entries

print all entries

Ligand N-Methyl-L-leucine

Basic Ligand Information

Molecular Structure

close all tables

open all tables

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (4 results)

top print hide

Show Substrate (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.4.3.3

N-methyl-DL-Leu + H2O + O2 = 4-methyl-2-oxopentanoate + methylamine + H2O2

741626

-

1.4.3.3

N-methyl-DL-leucine + H2O + O2 = ?

684598

-

1.4.3.3

2 entries

1.5.3.1

N-methyl-L-leucine + O2 + H2O = formaldehyde + L-leucine + H2O2

392378, 392376, 765379

-

1.5.8.3

N-methyl-L-leucine + acceptor + H2O = L-leucine + formaldehyde + reduced acceptor

392502

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

top print hide

Show Turnover Number (1 entry)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.5.3.1

0.0537

-

wild-type, 37°C, pH not specified in the publication

765379

K_M Value (4 results)

top print hide

Show KM Value (4 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.5.3.1

0.58

-

-

392376

1.5.3.1

106

-

-

392378

1.5.3.1

168.5

-

wild-type, 37°C, pH not specified in the publication

765379

1.5.3.1

3 entries

1.5.8.3

20

-

-

392502

References & Links

Literature References (6)

top print hide

Show Reference (6 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

392376

3435943

Purification and characterization of sarcosine oxidase of Streptomyces origin

1987

Inouye, Y.; Nishimura, M.; Matsuda, Y.; Hoshika, H.; Iwasaki, H.; Hujimura, K.; Asano, K.; Nakamura, S.

Chem. Pharm. Bull.

35

4194-4202

392378

3594681

Purification and characterization of sarcosine oxidase of Bacillus origin

1987

Matsuda, Y.; Hoshika, H.; Inouye, Y.; Ikuta, S.; Matsuura, K.; Nakamura, S.

Chem. Pharm. Bull.

35

711-717

392502

-

Sarcosine dehydrogenase from Pseudomonas putida: purification and some properties

1979

Oka, I.; Yoshimoto, T.; Rikitake, K.; Ogushi, S.; Tsur, D.

Agric. Biol. Chem.

43

1197-1203

684598

17279391

Overproduction and characterization of a recombinant D-amino acid oxidase from Arthrobacter protophormiae

2007

Geueke, B.; Weckbecker, A.; Hummel, W.

Appl. Microbiol. Biotechnol.

74

1240-1247

741626

25900453

A novel D-amino acid oxidase from a contaminated agricultural soil metagenome and its characterization

2015

Ou, Q.; Liu, Y.; Deng, J.; Chen, G.; Yang, Y.; Shen, P.; Wu, B.; Jiang, C.

Antonie van Leeuwenhoek

107

1615-1623

765379

-

Soluble expression of Thermomicrobium roseum sarcosine oxidase and characterization of N-demethylation activity

2019

Xin, Y.; Hao, M.; Fan, G.; Zhang, Y.; Zhang, L.

Mol. Catal.

464

48-56

Links to other databases for N-Methyl-L-leucine

top print hide

Show Links (1 entry)

ChEBI

PubChem