Ligand thio-NADP+

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Basic Ligand Information

Molecular Structure
Picture of thio-NADP+ (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C21H28N7O16P3S
thio-NADP+
OJNFDOAQUXJWED-NNYOXOHSSA-N
Synonyms:
beta-thio-NADP+, thio-NAD(P)+, thioNADP+, thionicotinamide-NADP+, thionicotinamide adenine dinucleotide phosphate

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (15 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
D-mannitol + thio-NADP+ = D-fructose + thio-NADPH
show the reaction diagram
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20alpha-hydroxypregn-4-en-3-one + thionicotinamide adenine dinucleotide phosphate = ?
show the reaction diagram
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D-glucose 6-phosphate + thionicotinamide-NADP+ = 6-phospho-D-glucono-1,5-lactone + thionicotinamide-NADPH + H+
show the reaction diagram
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D-glucose 6-phosphate + thio-NAD(P)+ = D-glucono-1,5-lactone 6-phosphate + thio-NAD(P)H
show the reaction diagram
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acetaldehyde + thio-NADP+ = acetate + ?
show the reaction diagram
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NADPH + H+ + thio-NADP+ = NADP+ + thio-NADPH + H+
show the reaction diagram
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thioNADP+ + H2O = ?
show the reaction diagram
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thio-NADP+ + H2O = ?
show the reaction diagram
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Product in Enzyme-catalyzed Reactions (6 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
2-oxopantoate + thio-NADPH = (R)-pantoate + thio-NADP+
show the reaction diagram
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2,3-dihydrodipicolinate + thio-NADPH + H+ = 2,3,4,5-tetrahydrodipicolinate + thio-NADP+ + H2O
show the reaction diagram
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L-glutamine + 2-oxoglutarate + thio-NADPH + H+ = L-glutamate + thio-NADP+
show the reaction diagram
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thio-NADPH + NAD+ + H+[side 1] = thio-NADP+ + NADH + H+[side 2]
show the reaction diagram
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Enzyme Cofactor/Cosubstrate (3 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
coenzyme analog
-
best cofactor
-

Inhibitor in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
65% inactivation at 0.2 mM
-
wild-type and mutant E201D and E201Q
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3D Structure of Enzyme-Ligand-Complex (PDB) (26 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (1 result)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
8000
-
pH 7.4, 80°C, recombinant enzyme

KM Value (18 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.00186
-
NADP analog
0.001
-
pH 7.8, 25°C
0.06
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pH 7.4, 80°C, recombinant enzyme
0.03
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cosubstrate NADH
0.004
0.015
recombinant mutants C53S, C53A, C58S
0.01
-
-

Ki Value (4 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.00023
-
-

References & Links

Links to other databases for thio-NADP+

ChEBI
PubChem
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PubChem