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Ligand L-asparaginyl-tRNAAsn

Basic Ligand Information

Molecular Structure

Show all BRENDA pathways known for L-asparaginyl-tRNAAsn

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Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (5 results)

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Show Natural Product (5 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

6.1.1.22

ATP + L-asparagine + tRNAAsn = AMP + diphosphate + L-asparaginyl-tRNAAsn

653175, 0, 653372, 653373, 650960

-

6.3.5.6

Asp-tRNAAsn + ? = Asn-tRNAAsn + ?

440094, 440095, 440090, 440092, 440093, 440091, 440087

-

6.3.5.6

ATP + Asp-tRNAAsn + L-glutamine + H2O = ADP + phosphate + Asn-tRNAAsn + L-glutamate

0

-

6.3.5.6

ATP + L-aspartyl-tRNAAsn + L-glutamine = ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate

0

-

6.3.5.6

3 entries

6.3.5.7

ATP + Asp-tRNAAsn + L-glutamine = ADP + phosphate + Asn-tRNAAsn + L-glutamate

0

-

Product in Enzyme-catalyzed Reactions (10 results)

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Show Product (10 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

6.1.1.12

ATP + L-asparagine + tRNAAsp = AMP + diphosphate + L-asparaginyl-tRNAAsp

652847

-

6.1.1.22

ATP + L-asparagine + tRNAAsn = AMP + diphosphate + L-asparaginyl-tRNAAsn

392, 386, 653175, 0, 114, 385, 387, 388, 391, 92, 390, 91, 653372, 653373, 650960

-

6.3.5.6

Asp-tRNAAsn + ? = Asn-tRNAAsn + ?

440094, 440095, 440090, 440092, 440093, 440091, 440087

-

6.3.5.6

ATP + Asp-tRNAAsn + glutamine = ADP + phosphate + Asn-tRNAAsn + glutamate

440087, 440090, 440094, 440095, 440093, 440091, 0, 440092

-

6.3.5.6

ATP + Asp-tRNAAsn + L-glutamine + H2O = ADP + phosphate + Asn-tRNAAsn + L-glutamate

0

-

6.3.5.6

ATP + Asp-tRNAAsn + L-glutamine = ADP + phosphate + Asn-tRNAAsn + L-glutamate

0

-

6.3.5.6

ATP + L-aspartyl-tRNAAsn + L-glutamine + H2O = ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate

0

-

6.3.5.6

ATP + L-aspartyl-tRNAAsn + L-glutamine = ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate

0

-

6.3.5.7

ATP + Asp-tRNAAsn + L-glutamine = ADP + phosphate + Asn-tRNAAsn + L-glutamate

492308, 0

-

6.3.5.7

ATP + L-aspartyl-tRNAAsn + L-glutamine + H2O = ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate

0

-

6.3.5.6

6 entries

6.3.5.7

2 entries

Enzyme Kinetic Parameters

References & Links

Literature References (23)

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Show Reference (23 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

91

690138

Purification and some properties of asparagine, lysine, serine, and valine:tRNA ligases from Bacillus stearothermophilus

1978

Samuelsson, T.; Lundvik, L.

J. Biol. Chem.

253

7033-7039

92

6849897

Molecular weights of mitochondrial and cytoplasmic aminoacyl-tRNA synthetases of beef liver and their complexes

1983

Walker, E.J.; Treacy, G.B.; Jeffrey, P.D.

Biochemistry

22

1934-1941

114

-

Amino acid substrate specificity of asparaginyl-, aspartyl- and glutaminyl-tRNA synthetase isolated from higher plants

1973

Lea, P.J.; Fowden, L.

Phytochemistry

12

1903-1916

385

8706760

Asparaginyl-tRNA synthetase from Thermus thermophilus HB8. Sequence of the gene and crystallization of the enzyme expressed in Escherichia coli

1996

Seignovert, L.; Härtlein, M.; Leberman, R.

Eur. J. Biochem.

239

501-508

386

7589498

An immunodominant antigen of Brugia malayi is an asparaginyl-tRNA synthetase

1995

Kron, M.; Marquard, K.; Härtlein, M.; Price, S.; Leberman, R.

FEBS Lett.

374

122-124

387

1544480

Asparaginyl-tRNA synthetase from the Escherichia coli temperature-sensitive strain HO202. A proline replacement in motif 2 is responsible for a large increase in Km for asparagine and ATP

1992

Madern, D.; Anselme, J.; Härtlein, M.

FEBS Lett.

299

85-89

388

2009959

Tyr-426 of the Escherichia coli asparaginyl-tRNA synthetase, an amino acid in a C-terminal conserved motif, is involved in ATP binding

1991

Anselme, J.; Härtlein, M.

FEBS Lett.

280

163-166

390

164948

Enhancement of the activity of asparaginyl-tRNA synthetase by an activator from rabbit liver

1975

Davies, M.R.; Marshall, R.D.

Biochim. Biophys. Acta

390

94-104

391

334719

Identification of a temperature-sensitive asparaginyl-transfer ribonucleic acid synthetase mutant of Escherichia coli

1977

Yamamoto, M.; Nomura, M.; Ohsawa, H.; Maruo, B.

J. Bacteriol.

132

127-131

392

618867

Biochemical characterization of a mutant asparaginyl-tRNA synthetase from Chinese hamster ovary cells

1978

Andrulis, I.L.; Chiang, C.S.; Arfin, S.M.; Miner, T.A.; Hatfield, G.W.

J. Biol. Chem.

253

58-62

440087

9789001

Glutamyl-tRNAGln amidotransferase in Deinococcus radiodurans may be confined to asparagine biosynthesis

1998

Curnow, A.W.; Tumbula, D.L.; Pelaschier, J.T.; Min, B.; Söll, D.

Proc. Natl. Acad. Sci. USA

95

12838-12843

440090

10913601

The heterotrimeric Thermus thermophilus Asp-tRNA(Asn) amidotransferase can also generate Gln-tRNA(Gln)

2000

Becker, H.D.; Min, B.; Jacobi, C.; Raczniak, G.; Pelaschier, J.; Roy, H.; Klein, S.; Kern, D.; Söll, D.

FEBS Lett.

476

140-144

440091

12149259

Evolutionary divergence of the archaeal aspartyl-tRNA synthetases into discriminating and nondiscriminating forms

2002

Tumbula-Hansen, D.; Feng, L.; Toogood, H.; Stetter, K.O.; Söll, D.

J. Biol. Chem.

277

37184-37190

440092

11585842

A single amidotransferase forms asparaginyl-tRNA and glutaminyl-tRNA in Chlamydia trachomatis

2001

Raczniak, G.; Becker, H.D.; Min, B.; Söll, D.

J. Biol. Chem.

276

45862-45867

440093

11880622

Transfer RNA-dependent amino acid biosynthesis: An essential route to asparagine formation

2002

Min, B.; Pelaschier, J.T.; Graham, D.E.; Tumbula-Hansen, D.; Söll, D.

Proc. Natl. Acad. Sci. USA

99

2678-2683

440094

10966471

Aminoacyl-tRNA synthesis

2000

Ibba, M.; Söll, D.

Annu. Rev. Biochem.

69

617-650

440095

10993083

Domain-specific recruitment of amide amino acids for protein synthesis

2000

Tumbula, D.L.; Becker, H.D.; Chang, W.Z.; Söll, D.

Nature

407

106-110

492308

11445070

A dual-specific Glu-tRNA(Gln) and Asp-tRNA(Asn) amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans

2001

Salazar, J.C.; Zuniga, R.; Raczniak, G.; Becker, H.; Soll, D.; Orellana, O.

FEBS Lett.

500

129-131

650960

9582288

The crystal structure of asparaginyl-tRNA synthetase from Thermus thermophilus and its complexes with ATP and asparaginyl-adenylate: the mechanism of discrimination between asparagine and aspartic acid

1998

Berthet-Colominas, C.; Seignovert, L.; Hartlein, M.; Grotli, M.; Cusack, S.; Leberman, R.

EMBO J.

17

2947-2960

652847

11237602

Binding free energies and free energy components from molecular dynamics and Poisson-Boltzmann calculations. Application to amino acid recognition by aspartyl-tRNA synthetase

2001

Archontis, G.; Simonson, T.; Karplus, M.

J. Mol. Biol.

306

307-327

653175

12798504

Expression, localization and alternative function of cytoplasmic asparaginyl-tRNA synthetase in Brugia malayi

2003

Kron, M.; Petridis, M.; Milev, Y.; Leykam, J.; Hartlein, M.

Mol. Biochem. Parasitol.

129

33-39

653372

9801298

Human asparaginyl-tRNA synthetase: molecular cloning and the inference of the evolutionary history of Asx-tRNA synthetase family

1998

Shiba, K.; Motegi, H.; Yoshida, M.; Noda, T.

Nucleic Acids Res.

26

5045-5051

653373

9421509

Human cytosolic asparaginyl-tRNA synthetase: cDNA sequence, functional expression in Escherichia coli and characterization as human autoantigen

1998

Beaulande, M.; Tarbouriech, N.; Hartlein, M.

Nucleic Acids Res.

26

521-524

Links to other databases for L-asparaginyl-tRNAAsn

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