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Ligand [E2 ubiquitin-conjugating enzyme]-L-cysteine

Basic Ligand Information

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.2.23

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine

734357, 737299, 736181, 737253, 735453, 758713, 759030, 759535, 758749, 759768, 759820, 758741, 758765, 760168, 759600, 760224, 759492, 758642, 759478, 759764, 736063, 725526, 759124

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In Vivo Product in Enzyme-catalyzed Reactions (7 results)

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Show Natural Product (7 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [SDIRIP1 protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[SDIRIP1 protein]-L-lysine

0

-

2.3.2.31

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.31

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine

0

-

2.3.2.33

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine

0

-

2.3.2.33

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RCR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine

0

-

2.3.2.27

2 entries

2.3.2.31

2 entries

2.3.2.33

2 entries

2.3.2.36

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine

0

-

Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Substrate (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.2.23

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine

734357, 737299, 736181, 737253, 735453, 758713, 759030, 759535, 758749, 759768, 759820, 758741, 758765, 760168, 759600, 760224, 759492, 758642, 759478, 759764, 736063, 725526, 759124

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Product in Enzyme-catalyzed Reactions (38 results)

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Show Product (38 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.3.2.26

S-(ubiquitin)n-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [adenomatous polyposis coli]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-(ubiquitin)n-[adenomatous polyposis coli]-L-lysine

0

-

2.3.2.26

S-(ubiquitin)n-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [Dvl2]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-(ubiquitin)n-[Dvl2]-L-lysine

0

-

2.3.2.26

S-(ubiquitin)n-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [Glis3]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-(ubiquitin)n-[Glis3]-L-lysine

0

-

2.3.2.26

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [caspase-8]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[caspase-8]-L-lysine

0

-

2.3.2.26

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [ING2]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[ING2]-L-lysine

0

-

2.3.2.26

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [Sav]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[Sav]-L-lysine

0

-

2.3.2.26

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [Spry2]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[Spry2]-L-lysine

0

-

2.3.2.26

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [ubiquitin]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[ubiquitin]-L-lysine

0

-

2.3.2.26

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [Dvl2]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [Dvl2]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.26

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [my-opioid receptor MOR1]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [my-opioid receptor MOR1]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.26

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [Spo12]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [Spo12]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.26

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [Ubl4A]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [Ubl4A]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.27

S-(ubiquitin)n-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [POX1]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-(ubiquitin)n-[POX1]-L-lysine

0

-

2.3.2.27

S-(ubiquitin)n-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [RNF186]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-(ubiquitin)n-[RNF186]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [ABI5]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[ABI5]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [ACS4]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[ACS4]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [ACS7]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[ACS7]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [bHLH065]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[bHLH065]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [BNip1]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[BNip1]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [GRP1]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[GRP1]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HCI1]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[HCI1]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [histone H2A]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[histone H2A]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [PGLU1]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[PGLU1]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [Ring1b]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[Ring1b]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [SDIRIP1 protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[SDIRIP1 protein]-L-lysine

0

-

2.3.2.27

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [TIP4.1]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[TIP4.1]-L-lysine

0

-

2.3.2.27

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [betaC1 protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [betaC1 protein]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.27

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [FANCD2]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [FANCD2]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.27

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [KAT1]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [KAT1]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.27

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [MLO12-Myc]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor MLO12-Myc]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.27

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [MYB1]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [MYB1]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.31

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine

0

-

2.3.2.31

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine

0

-

2.3.2.33

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-threonine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-3-O-ubiquitinyl-L-threonine

0

-

2.3.2.33

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [F-box protein FBXW7]-L-threonine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [F-box protein FBXW7]-3-O-ubiquitinyl-L-threonine

0

-

2.3.2.33

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RCR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RCR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine

0

-

2.3.2.26

12 entries

2.3.2.27

20 entries

2.3.2.31

2 entries

2.3.2.33

3 entries

2.3.2.36

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine

0

-

Enzyme Kinetic Parameters

References & Links

Literature References (23)

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Show Reference (23 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

725526

23696636

The ubiquitin-conjugating enzyme (E2) Ube2w ubiquitinates the N terminus of substrates

2013

Scaglione, K.M.; Basrur, V.; Ashraf, N.S.; Konen, J.R.; Elenitoba-Johnson, K.S.; Todi, S.V.; Paulson, H.L.

J. Biol. Chem.

288

18784-18788

734357

24928900

A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently

2014

Fiesel, F.; Moussaud-Lamodiere, E.; Ando, M.; Springer, W.

J. Cell Sci.

127

3488-3504

735453

23918348

Serine/threonine kinases and E2-ubiquitin conjugating enzymes in Planctomycetes: unexpected findings

2013

Arcas, A.; Cases, I.; Rojas, A.

Antonie van Leeuwenhoek

104

509-520

736063

26283706

Spectrin's chimeric E2/E3 enzymatic activity

2015

Goodman, S.; Petrofes Chapa, R.; Zimmer, W.

Exp. Biol. Med.

240

1039-1049

736181

26927519

A newly discovered ubiquitin-conjugating enzyme E2 correlated with the cryogenic autolysis of Volvariella volvacea

2016

Gong, M.; Wang, H.; Chen, M.; Bao, D.; Zhu, Q.; Tan, Q.

Gene

583

58-63

737253

26525192

Biochemical and structural characterization of a novel ubiquitin-conjugating enzyme E2 from Agrocybe aegeria reveals Ube2w family-specific properties

2015

Qi, C.; Li, D.F.; Feng, L.; Hou, Y.; Sun, H.; Wang, D.C.; Liu, W.

Sci. Rep.

5

16056

737299

26135843

Cellular Ubc2/Rad6 E2 ubiquitin-conjugating enzyme facilitates tombusvirus replication in yeast and plants

2015

Imura, Y.; Molho, M.; Chuang, C.; Nagy, P.D.

Virology

484

265-275

758642

27038327

Insights into the allosteric inhibition of the SUMO E2 enzyme Ubc9

2016

Hewitt, W.M.; Lountos, G.T.; Zlotkowski, K.; Dahlhauser, S.D.; Saunders, L.B.; Needle, D.; Tropea, J.E.; Zhan, C.; Wei, G.; Ma, B.; Nussinov, R.; Waugh, D.S.; Schneekloth, J.S.

Angew. Chem. Int. Ed. Engl.

55

5703-5707

758713

30658189

Differential use of E2 ubiquitin conjugating enzymes for regulated degradation of the rate-limiting enzymes HMGCR and SQLE in cholesterol biosynthesis

2019

Tan, J.M.E.; Cook, E.C.L.; van den Berg, M.; Scheij, S.; Zelcer, N.; Loregger, A.

Atherosclerosis

281

137-142

758741

26718580

DNA damage-induced foci of E2 ubiquitin-conjugating enzyme are detectable upon co-transfection with an interacting E3 ubiquitin ligase

2016

Wang, D.; Tian, Y.; Wei, D.; Jing, Y.; Niu, H.; Xie, K.; Song, Y.

Biochem. Genet.

54

147-157

758749

27729585

Mechanism and disease association of E2-conjugating enzymes lessons from UBE2T and UBE2L3

2016

Alpi, A.F.; Chaugule, V.; Walden, H.

Biochem. J.

473

3401-3419

758765

30449727

Ubiquitin-conjugating enzyme E2 B regulates the ubiquitination of O6-methylguanine-DNA methyltransferase and BCNU sensitivity in human nasopharyngeal carcinoma cells

2018

Hsu, S.H.; Chen, S.H.; Kuo, C.C.; Chang, J.Y.

Biochem. Pharmacol.

158

327-338

759030

27002219

E2 enzymes more than just middle men

2016

Stewart, M.D.; Ritterhoff, T.; Klevit, R.E.; Brzovic, P.S.

Cell Res.

26

423-440

759124

31523916

The ubiquitin E2 enzyme UBE2QL1 mediates lysophagy

2019

Mizushima, N.

EMBO Rep.

20

e49104

759478

26555268

Structure of UBE2Z enzyme provides functional insight into specificity in the FAT10 protein conjugation machinery

2016

Schelpe, J.; Monte, D.; Dewitte, F.; Sixma, T.; Rucktooa, P.

J. Biol. Chem.

291

630-639

759492

29414787

Ubiquitin-conjugating enzyme E2 D1 (Ube2D1) mediates lysine-independent ubiquitination of the E3 ubiquitin ligase March-I

2018

Lei, L.; Bandola-Simon, J.; Roche, P.A.

J. Biol. Chem.

293

3904-3912

759535

28743740

Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation

2017

van de Weijer, M.L.; Schuren, A.B.C.; van den Boomen, D.J.H.; Mulder, A.; Claas, F.H.J.; Lehner, P.J.; Lebbink, R.J.; Wiertz, E.J.H.J.

J. Cell Sci.

130

2883-2892

759600

28437106

Allosteric targeting of the Fanconi anemia ubiquitin-conjugating enzyme Ube2T by fragment screening

2017

Morreale, F.E.; Bortoluzzi, A.; Chaugule, V.K.; Arkinson, C.; Walden, H.; Ciulli, A.

J. Med. Chem.

60

4093-4098

759764

27570074

Posttranscriptional regulation of glycoprotein quality control in the endoplasmic reticulum is controlled by the E2 Ub-conjugating enzyme UBC6e

2016

Hagiwara, M.; Ling, J.; Koenig, P.A.; Ploegh, H.L.

Mol. Cell

63

753-767

759768

27044868

Mechanism of lysine 48 selectivity during polyubiquitin chain formation by the Ube2R1/2 ubiquitin-conjugating enzyme

2016

Hill, S.; Harrison, J.S.; Lewis, S.M.; Kuhlman, B.; Kleiger, G.

Mol. Cell. Biol.

36

1720-1732

759820

31341161

Structural insights into E1 recognition and the ubiquitin-conjugating activity of the E2 enzyme Cdc34

2019

Williams, K.M.; Qie, S.; Atkison, J.H.; Salazar-Arango, S.; Alan Diehl, J.; Olsen, S.K.

Nat. Commun.

10

3296

760168

29725124

Active site gate dynamics modulate the catalytic activity of the ubiquitination enzyme E2-25K

2018

Rout, M.K.; Lee, B.L.; Lin, A.; Xiao, W.; Spyracopoulos, L.

Sci. Rep.

8

7002

760224

27020591

Ubiquitin-conjugating enzyme E2T is an independent prognostic factor and promotes gastric cancer progression

2016

Yu, H.; Xiang, P.; Pan, Q.; Huang, Y.; Xie, N.; Zhu, W.

Tumour Biol.

37

11723-11732

Links to other databases for [E2 ubiquitin-conjugating enzyme]-L-cysteine

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