Ligand L-Ala-L-Ala-L-Phe 4-nitroanilide
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Basic Ligand Information
Molecular Structure
C21H25N5O5
L-Ala-L-Ala-L-Phe 4-nitroanilide
NEQHCLFEDFICRC-IYOUNJFTSA-O
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (1 result)
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L-Ala-L-Ala-L-Phe 4-nitroanilide + H2O = L-Ala-L-Ala-L-Phe + 4-nitroaniline
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (30 results)
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0.38
-
mutant H267A, pH 6.4, 37°C
0.441
-
mutant H267A, pH 8.3, 37°C
0.86
-
mutant H267A, pH 7.9, 37°C
0.9
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mutant H267A, pH 6.8, 37°C
0.9
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mutant H267A, pH 7.6, 37°C
1
-
mutant D387G, pH 8.3, 37°C
3
-
mutant D387G, pH 7.9, 37°C
4
-
mutant E331Q, pH 7.9, 37°C
4
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mutant E331Q, pH 8.3, 37°C
5
-
mutant D387G, pH 6.4, 37°C
6
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mutant D387G, pH 7.6, 37°C
7.2
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mutant D387G, pH 6.8, 37°C
18
-
mutant E331Q, pH 7.6, 37°C
38
-
wild-type, pH 6.4, 37°C
42
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wild-type, pH 6.4, 37°C
50
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wild-type, pH 6.8, 37°C
57
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wild-type, pH 7.6, 37°C
58.8
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wild-type, pH 6.8, 37°C
60
-
wild-type, pH 7.9, 37°C
65
-
wild-type, pH 8.3, 37°C
80
-
mutant E331Q, pH 6.8, 37°C
96
-
wild-type, pH 7.6, 37°C
114
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wild-type, pH 7.9, 37°C
125
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wild-type, pH 8.3, 37°C
500
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mutant E331Q, pH 6.4, 37°C
KM Value (30 results)
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0.017
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mutant H267A, pH 6.8, 37°C
0.024
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mutant H267A, pH 6.4, 37°C
0.028
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mutant H267A, pH 7.6, 37°C
0.037
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mutant H267A, pH 7.9, 37°C
0.054
-
mutant H267A, pH 8.3, 37°C
0.056
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wild-type, pH 7.6, 37°C
0.058
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wild-type, pH 7.6, 37°C
0.062
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wild-type, pH 8.3, 37°C
0.07
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wild-type, pH 6.8, 37°C
0.074
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wild-type, pH 7.9, 37°C
0.09
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wild-type, pH 6.8, 37°C
0.09
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wild-type, pH 7.9, 37°C
0.1
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wild-type, pH 6.4, 37°C
0.11
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mutant D387G, pH 8.3, 37°C
0.12
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wild-type, pH 8.3, 37°C
0.2
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mutant D387G, pH 6.4, 37°C
0.21
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wild-type, pH 6.4, 37°C
0.25
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mutant D387G, pH 7.6, 37°C
0.27
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mutant D387G, pH 7.9, 37°C
0.3
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mutant D387G, pH 6.8, 37°C
0.5
-
mutant E331Q, pH 7.9, 37°C
0.6
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mutant E331Q, pH 8.3, 37°C
0.9
-
mutant E331Q, pH 7.6, 37°C
6
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mutant E331Q, pH 6.8, 37°C
30
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mutant E331Q, pH 6.4, 37°C
References & Links
Literature References (1)
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Exploring the active site of tripeptidyl-peptidase II through studies of pH dependence of reaction kinetics
2012
Eklund, S.; Lindas, A.C.; Hamnevik, E.; Widersten, M.; Tomkinson, B.
Biochim. Biophys. Acta
1824
561-570
Links to other databases for L-Ala-L-Ala-L-Phe 4-nitroanilide
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