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Ligand 8-azido-GTP Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C1 0 H1 5 N8 O1 4 P3
8-azido-GTP
JOEORHHXHRXYIW-UMMCILCDSA-N
8-azidoguanosine 5'-triphosphate
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (3 results)
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8-Azido-GTP + glucose 1-phosphate = 8-Azido-GDPglucose + diphosphate
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8-Azido-GTP + mannose 1-phosphate = 8-Azido-GDP mannose + diphosphate
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8-azido-GTP + H2O = 8-azido-GDP + phosphate
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Inhibitor in Enzyme-catalyzed Reactions (3 results)
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used for affinity photolabeling, 0.1 mM, 95% inhibition
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0.6 mM, 91% inhibition of GTP cyclohydrolase activity, 0.3 mM, 53% inhibition of diphosphate phosphohydrolase activity
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photoinactivation is caused by formation of an intramolecular cystine disulfide bridge
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Enzyme Kinetic Parameters
Ki Value (1 result)
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References & Links Literature References (5)
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Formation of an intramolecular cystine disulfide during the reaction of 8-azidoguanosine 5'-triphosphate with cytosolic phosphoenolpyruvate carboxykinase (GTP) causes inactivation without photolabeling
1989
Lewis, C.T.; Haley, B.E.; Carlson, G.M.
Biochemistry
28
9248-9255
Identification of a guanine binding domain peptide of the GTP binding site of glutamate dehydrogenase: Isolation with metal-chelate affinity chromatography
1993
Shoemaker, M.T.; Haley, B.E.
Biochemistry
32
1883-1890
A member of a new class of GTP cyclohydrolases produces formylaminopyrimidine nucleotide monophosphates
2002
Graham, D.E.; Xu, H.; White, R.H.
Biochemistry
41
15074-15084
GDP-mannose pyrophosphorylase. Purification to homogeneity, properties, and utilization to prepare photoaffinity analogs
1993
Szumilo, T.; Drake, R.R.; York, J.L.; Elbein, A.D.
J. Biol. Chem.
268
17943-17950
The mitotic apparatus-associated 51-kDa protein from sea urchin eggs is a GTP-binding protein and is immunologically related to yeast polypeptide elongation factor 1alpha
1990
Ohta, K.; Toriyama, M.; Miyazaki, M.; Murofushi, H.; Hosoda, S.; Endo, S.; Sakai, H.
J. Biol. Chem.
265
3240-3247
Links to other databases for 8-azido-GTP
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