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Ligand 8-amino-8-demethylriboflavin

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

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Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.1.1.343

8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine = roseoflavin + S-adenosyl-L-homocysteine

715642

-

2.1.1.343

S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin = S-adenosyl-L-homocysteine + roseoflavin

742838, 742498

-

2.1.1.343

2 entries

Substrate in Enzyme-catalyzed Reactions (3 results)

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Show Substrate (3 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.1.1.343

8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine = roseoflavin + S-adenosyl-L-homocysteine

715642

-

2.1.1.343

S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin = S-adenosyl-L-homocysteine + roseoflavin

742838, 742498

-

2.1.1.343

2 entries

2.7.1.26

ATP + 8-aminoriboflavin = ADP + 8-aminoriboflavin monophosphate

739570

-

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.7.1.26

-

641241

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (3 results)

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Show Turnover Number (3 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

2.1.1.343

0.001

-

at pH 8.0 and 25°C

742498

2.1.1.343

0.37

-

at pH 8.0 and 37°C

742838

2.1.1.343

0.37

-

pH 8.0, 37°C

715642

2.1.1.343

3 entries

K_M Value (3 results)

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Show KM Value (3 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.1.1.343

0.004

-

at pH 8.0 and 25°C

742498

2.1.1.343

0.0577

-

apparent value, at pH 8.0 and 37°C

742838

2.1.1.343

0.0577

-

pH 8.0, 37°C

715642

2.1.1.343

3 entries

K_i Value (1 result)

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Show KI Value (1 entry)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.7.1.26

0.25

-

pH 8, 37°C

641241

References & Links

Literature References (5)

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Show Reference (5 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

641241

6243635

Affinity chromatographic purification and properties of flavokinase (ATP:riboflavin 5-phosphotransferase) from rat liver

1980

Merrill, A.H.; McCormick, D.B.

J. Biol. Chem.

255

1335-1338

715642

21911488

A novel N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis

2011

Jankowitsch, F.; Kühm, C.; Kellner, R.; Kalinowski, J.; Pelzer, S.; Macheroux, P.; Mack, M.

J. Biol. Chem.

286

38275-285

739570

24170887

Truncated FAD synthetase for direct biocatalytic conversion of riboflavin and analogs to their corresponding flavin mononucleotides

2013

Iamurri, S.M.; Daugherty, A.B.; Edmondson, D.E.; Lutz, S.

Protein Eng. Des. Sel.

26

791-795

742498

26913589

Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-D-riboflavin to the antibiotic roseoflavin

2016

Tongsook, C.; Uhl, M.; Jankowitsch, F.; Mack, M.; Gruber, K.; Macheroux, P.

FEBS J.

283

1531-1549

742838

21911488

A novel N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis

2011

Jankowitsch, F.; Kühm, C.; Kellner, R.; Kalinowski, J.; Pelzer, S.; Macheroux, P.; Mack, M.

J. Biol. Chem.

286

38275-38285

Links to other databases for 8-amino-8-demethylriboflavin

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Show Links (1 entry)

ChEBI

PubChem