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BRENDA support

Ligand Selenite

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Basic Ligand Information

Molecular Structure
Picture of Selenite (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
H2O3Se
Selenite
MCAHWIHFGHIESP-UHFFFAOYSA-L
Synonyms:
(SeO3)2-, SeO32-
Pathway Source
Pathways

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
selenite + NADH + H+ = selenium + H2O + NAD+
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE

Substrate in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
(SeO3)2- + H2 + ? = Se + H2O + ?
show the reaction diagram
-
SeO32- + NADPH + H+ = ?
show the reaction diagram
-
selenite + ferricyanide + H2O = ? + ferrocyanide
show the reaction diagram
-
SeO32- + a [DsrC protein]-dithiol + 2 reduced methyl viologen + 2 H+ = HSe- + a [DsrC protein]-disulfide + 2 oxidized methyl viologen + 3 H2O
show the reaction diagram
-
selenite + NADH + H+ = selenium + H2O + NAD+
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (13 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
selenate + reduced methylviologen = selenite + H2O + oxidized methylviologen
show the reaction diagram
-

Activator in Enzyme-catalyzed Reactions (2 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
4fold increase in activity in U-1256 cells, less pronounced in presence of doxorubicin, differences between drug-sensitive and drug-resistant cells, overview
-

Inhibitor in Enzyme-catalyzed Reactions (13 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
inactivates IDPm
-
20% inhibition at 0.1 mg/l
-
selenite induces topoisomerase I-DNA complexes in K562 cells
-
selenite induces topoisomerase II-DNA complexes in K562 cells
-
competitively inhibits phosphate uptake; competitively inhibits phosphate uptake; competitively inhibits phosphate uptake; competitively inhibits phosphate uptake
-

Metals and Ions (2 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
selenium treatment results in increased expression of almost all TrxR1 mRNA variants with increasing concentrations of selenite
-
one of the activating anions, that stimulates MgATPase activity of EcF1, likely due to a decrease in a relative content of MgADP-inhibited EcF1 because activating anions accelerate reactivation of MgADP-inhibited enzyme
-

3D Structure of Enzyme-Ligand-Complex (PDB) (1 result)

EC NUMBER
ENZYME 3D STRUCTURE

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (3 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE

KM Value (4 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
5.47
-
pH 6.0, 35°C

Ki Value (2 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE

References & Links

Links to other databases for Selenite

ChEBI
PubChem
ChEBI
PubChem