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2.6
-
pH not specified in the publication, temperature not specified in the publication
4.5
-
mutant A163C/S244C, at pH 7.5 and 32°C
5.5
-
mutant N261A, at pH 7.5 and 32°C
5.9
-
wild type enzyme, at pH 7.5 and 32°C
5.9
-
wild-type, pH 7.5, 32°C
6
-
mutant A163C, at pH 7.5 and 32°C
6.2
-
mutant S244C, at pH 7.5 and 32°C
6.8
-
mutant S205A, at pH 7.5 and 32°C
6.8
-
mutant S205A, pH 7.5, 32°C
6.9
-
mutant S167A, at pH 7.5 and 32°C
7.1
-
mutant P198A, at pH 7.5 and 32°C
7.1
-
mutant P198A, pH 7.5, 32°C
8
-
mutant F263Y, at pH 7.5 and 32°C
8
-
mutant F263Y, pH 7.5, 32°C
9.7
-
mutant V265T, at pH 7.5 and 32°C
16.2
-
mutant V265A, at pH 7.5 and 32°C
17.3
-
mutant S165A, at pH 7.5 and 32°C
17.3
-
mutant S165A, pH 7.5, 32°C
19.6
-
mutant F242Y, at pH 7.5 and 32°C
19.6
-
mutant F242Y, pH 7.5, 32°C
24.6
-
mutant V265L, at pH 7.5 and 32°C
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Functional expression of the ectoine hydroxylase gene (thpD) from Streptomyces chrysomallus in Halomonas elongata
2004
Prabhu, J.; Schauwecker, F.; Grammel, N.; Keller, U.; Bernhard, M.
Appl. Environ. Microbiol.
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3130-3132
Synthesis and uptake of the compatible solutes ectoine and 5-hydroxyectoine by Streptomyces coelicolor A3(2) in response to salt and heat stresses
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Bursy, J.; Kuhlmann, A.U.; Pittelkow, M.; Hartmann, H.; Jebbar, M.; Pierik, A.J.; Bremer, E.
Appl. Environ. Microbiol.
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A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581 T
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Osmotically induced synthesis of the compatible solute hydroxyectoine is mediated by an evolutionarily conserved ectoine hydroxylase
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31147-31155
Crystal structure of the ectoine hydroxylase, a snapshot of the active site
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Hoeppner, A.; Widderich, N.; Lenders, M.; Bremer, E.; Smits, S.H.
J. Biol. Chem.
289
29570-29583
Molecular dynamics simulations and structure-guided mutagenesis provide insight into the architecture of the catalytic core of the ectoine hydroxylase
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Widderich, N.; Pittelkow, M.; Hoeppner, A.; Mulnaes, D.; Buckel, W.; Gohlke, H.; Smits, S.H.; Bremer, E.
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Biochemical properties of ectoine hydroxylases from extremophiles and their wider taxonomic distribution among microorganisms
2014
Widderich, N.; Hoeppner, A.; Pittelkow, M.; Heider, J.; Smits, S.H.; Bremer, E.
PLoS ONE
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Ectoine and 5-hydroxyectoine accumulation in the halophile Virgibacillus halodenitrificans PDB-F2 in response to salt stress
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Tao, P.; Li, H.; Yu, Y.; Gu, J.; Liu, Y.
Appl. Microbiol. Biotechnol.
100
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Strangers in the archaeal world osmostress-responsive biosynthesis of ectoine and hydroxyectoine by the marine thaumarchaeon Nitrosopumilus maritimus
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Widderich, N.; Czech, L.; Elling, F.J.; Koenneke, M.; Stoeveken, N.; Pittelkow, M.; Riclea, R.; Dickschat, J.S.; Heider, J.; Bremer, E.
Environ. Microbiol.
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Molecular dynamics simulations and structure-guided mutagenesis provide insight into the architecture of the catalytic core of the ectoine hydroxylase
2014
Widderich, N.; Pittelkow, M.; Höppner, A.; Mulnaes, D.; Buckel, W.; Gohlke, H.; Smits, S.; Bremer, E.
J. Mol. Biol.
426
586-600
Ectoine hydroxylase displays selective trans-3-hydroxylation activity towards L-proline
2019
Hara, R.; Nishikawa, T.; Okuhara, T.; Koketsu, K.; Kino, K.
Appl. Microbiol. Biotechnol.
103
5689-5698
Exploiting substrate promiscuity of ectoine hydroxylase for regio- and stereoselective modification of homoectoine
2019
Czech, L.; Wilcken, S.; Czech, O.; Linne, U.; Brauner, J.; Smits, S.H.J.; Galinski, E.A.; Bremer, E.
Front. Microbiol.
10
2745
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