Ligand L-cystinyl-p-nitroanilide
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Basic Ligand Information
C9H11N3O3S
L-cystinyl-p-nitroanilide
IREUBXFNRHMSNK-QMMMGPOBSA-N
Cys-4-nitroanilide, L-Cys-4-nitroanilide, L-cysteine-nitroanilide
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (3 results)
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L-Cys-4-nitroanilide + H2O = L-Cys + 4-nitroaniline
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L-cystinyl-p-nitroanilide + H2O = L-cystine + p-nitroaniline
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Cys-4-nitroanilide + H2O = Cys + 4-nitroaniline
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (2 results)
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3.71
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wild type enzyme, at pH 8.0 and 37°C
46.7
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mutant enzyme D198I/F221W, at pH 8.0 and 37°C
KM Value (4 results)
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0.056
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mutant enzyme D198I/F221W, at pH 8.0 and 37°C
0.11
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wild type enzyme, at pH 8.0 and 37°C
0.331
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pH 7.4, 37°C, wild-type enzyme
References & Links
Literature References (4)
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Purification and characterization of the cystinyl bond cleaving yeast aminopeptidase yscXVI
1993
Tisljar, U.; Wolf, D.H.
FEBS Lett.
322
191-196
Mutational analysis of the active site of human insulin-regulated aminopeptidase
2001
Laustsen, P.G.; Vang, S.; Kristensen, T.
Eur. J. Biochem.
268
98-104
A thermostable leucine aminopeptidase from Bacillus kaustophilus CCRC 11223
2004
Lin, L.L.; Hsu, W.H.; Wu, C.P.; Chi, M.C.; Chou, W.M.; Hu, H.Y.
Extremophiles
8
79-87
Change in substrate preference of Streptomyces aminopeptidase through modification of the environment around the substrate binding site
2006
Arima, J.; Uesugi, Y.; Iwabuchi, M.; Hatanaka, T.
Appl. Environ. Microbiol.
72
7962-7967
Links to other databases for L-cystinyl-p-nitroanilide
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