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Ligand ent-cassa-12,15-diene

Basic Ligand Information

Molecular Structure

Show all BRENDA pathways known for ent-cassa-12,15-diene

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.14.70

ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 = 3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O

728514

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In Vivo Product in Enzyme-catalyzed Reactions (1 result)

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Show Natural Product (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.2.3.28

ent-copalyl diphosphate = ent-cassa-12,15-diene + diphosphate

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Substrate in Enzyme-catalyzed Reactions (8 results)

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Show Substrate (8 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.14.112

ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2 = 11-(S)-hydroxy-entcassa-12,15-diene + [oxidized NADPH-hemoprotein reductase] + H2O

718211

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1.14.14.123

ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 = ?

717875

-

1.14.14.69

ent-cassa-12,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O

744147, 744895

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1.14.14.69

ent-cassa-12,15-diene + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O

744147

-

1.14.14.69

2 entries

1.14.14.70

ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 = 3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O

728514

-

1.14.14.86

ent-cassadiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = ent-cassadien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O

746311

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1.14.14.86

ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 = ?

746311

-

1.14.14.86

ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 = ent-cassadien-19-oic acid + [oxidized NADPH-hemoprotein reductase] + H2O

744583

-

1.14.14.86

3 entries

Product in Enzyme-catalyzed Reactions (1 result)

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Show Product (1 entry)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.2.3.28

ent-copalyl diphosphate = ent-cassa-12,15-diene + diphosphate

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Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (3 results)

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Show Turnover Number (3 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.14.14.123

0.006

-

pH and temperature not specified in the publication

717875

1.14.14.69

0.11

-

pH not specified in the publication, temperature not specified in the publication

744895

1.14.14.70

0.017

-

pH and temperature not specified in the publication

728514

K_M Value (6 results)

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Show KM Value (6 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.14.14.112

0.039

-

in 0.1 M Tris-HCl, pH 7.2, 20% (v/v) glycerol, 0.5 mM EDTA, 1 mM dithiothreitol, temperature not specified in the publication

718211

1.14.14.123

0.004

-

pH and temperature not specified in the publication

717875

1.14.14.69

0.2

-

pH not specified in the publication, temperature not specified in the publication

744895

1.14.14.70

0.002

-

pH and temperature not specified in the publication

728514

1.14.14.86

0.05

-

pH and temperature not specified in the publication

746311

1.14.14.86

0.05

-

pH not specified in the publication, temperature not specified in the publication

746311

1.14.14.86

2 entries

References & Links

Literature References (7)

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Show Reference (7 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

717875

22215681

Characterization of CYP76M5-8 indicates metabolic plasticity within a plant biosynthetic gene cluster

2012

Wang, Q.; Hillwig, M.; Okada, K.; Yamazaki, K.; Wu, Y.; Swaminathan, S.; Yamane, H.; Peters, R.

J. Biol. Chem.

287

6159-6168

718211

19825834

CYP76M7 is an ent-cassadiene C11 alpha-hydroxylase defining a second multifunctional diterpenoid biosynthetic gene cluster in rice

2009

Swaminathan, S.; Morrone, D.; Wang, Q.; Bruce Fulton, D.; Peters, R.

Plant Cell

21

3315-3325

728514

22247270

CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism

2012

Wang, Q.; Hillwig, M.L.; Wu, Y.; Peters, R.J.

Plant Physiol.

158

1418-1425

744147

25758958

Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis

2015

Kitaoka, N.; Wu, Y.; Xu, M.; Peters, R.J.

Appl. Microbiol. Biotechnol.

99

7549-7558

744583

28721585

CYP701A26 is characterized as an ent-kaurene oxidase with putative involvement in maize gibberellin biosynthesis

2017

Mao, H.; Shen, Q.; Wang, Q.

Biotechnol. Lett.

39

1709-1716

744895

21985968

Parsing a multifunctional biosynthetic gene cluster from rice Biochemical characterization of CYP71Z6 & 7

2011

Wu, Y.; Hillwig, M.L.; Wang, Q.; Peters, R.J.

FEBS Lett.

585

3446-3451

746311

26884192

Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis

2016

Mafu, S.; Jia, M.; Zi, J.; Morrone, D.; Wu, Y.; Xu, M.; Hillwig, M.L.; Peters, R.J.

Proc. Natl. Acad. Sci. USA

113

2526-2531

Links to other databases for ent-cassa-12,15-diene

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Show Links (1 entry)

ChEBI

PubChem

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