Ligand 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
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Basic Ligand Information
Molecular Structure

C10H18N5O15P3
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
NDXMRXXKCCKQQV-UUOKFMHZSA-N
Formamidopyrimidine nucleoside triphosphate, N-[2-amino-5-(formylamino)-6-oxo-1,6-dihydropyrimidin-4-yl]-beta-D-ribofuranosylamine 5'-triphosphate
Show all BRENDA pathways known for 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (1 result)
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2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate + H2O = ?
-
Enzyme Kinetic Parameters
kcat Value (Turnover Number) (3 results)
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0.05
-
wild type enzyme, at pH 8.5 and 30°C
0.09
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C
0.1
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mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C
KM Value (3 results)
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0.031
-
wild type enzyme, at pH 8.5 and 30°C
0.079
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C
0.122
-
mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C
References & Links
Literature References (2)
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Biosynthesis of riboflavin. Screening for an improved GTP cyclohydrolase II mutant
2009
Lehmann, M.; Degen, S.; Hohmann, H.P.; Wyss, M.; Bacher, A.; Schramek, N.
FEBS J.
276
4119-4129
Structures and reaction mechanisms of GTP cyclohydrolases
2013
Graewert, T.; Fischer, M.; Bacher, A.
IUBMB Life
65
310-322
Links to other databases for 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate