Ligand reduced methylene blue

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Basic Ligand Information

Molecular Structure
Picture of reduced methylene blue (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C16H19N3S
reduced methylene blue
QTWZICCBKBYHDM-UHFFFAOYSA-N

Roles as Enzyme Ligand

In Vivo Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
H2 + methylene blue = reduced methylene blue
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Substrate in Enzyme-catalyzed Reactions (5 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
phenylpyruvate + reduced methylene blue = (2R)-phenyllactate + oxidized methylene blue
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5,10-methylenetetrahydrofolate + reduced methylene blue = 5-methyltetrahydrofolate + methylene blue
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nitrate + reduced methylene blue = nitrite + H2O + oxidized methylene blue
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tetrathionate + reduced methylene blue = thiosulfate + oxidized methylene blue
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N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue = coenzyme B + coenzyme M + methylene blue
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Product in Enzyme-catalyzed Reactions (52 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
sn-glycerol 3-phosphate + methylene blue = glycerone phosphate + reduced methylene blue
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lactose + methylene blue = 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced methylene blue
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H2 + oxidized methylene blue = H+ + reduced methylene blue
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H2 + methylene blue = H+ + reduced methylene blue
show the reaction diagram
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H2 + oxidized methylene blue = H+ + reduced methylene blue
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formate + methylene blue = CO2 + reduced methylene blue
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xanthine + methylene blue + O2 = urate + reduced methylene blue
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6-hydroxynicotinate + H2O + methylene blue = 2,6-dihydroxynicotinate + H2O2 + reduced methylene blue
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formate + methylene blue = CO2 + reduced methylene blue
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CO + methyl viologen + H2O = CO2 + reduced methylene blue
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pyruvate + CoA + oxidized methylene blue = acetyl-CoA + CO2 + reduced methylene blue + H+
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benzaldehyde + H2O + methylene blue = benzoate + reduced methylene blue
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D-alanine + oxidized methylene blue + H2O = pyruvate + reduced methylene blue + H2O2
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5-methyltetrahydrofolate + methylene blue = 5,10-methylenetetrahydrofolate + reduced methylene blue
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(S)-nicotine + methylene blue + H2O = (S)-6-hydroxynicotine + reduced methylene blue
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NADH + H+ + oxidized methylene blue = NAD+ + reduced methylene blue
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methylene blue + NAD(P)H = reduced methylene blue + NAD(P)+
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methylene blue + NADH = reduced methylene blue + NAD+
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methylene blue + NADH + H+ = reduced methylene blue + NAD+
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coenzyme B + coenzyme M + methylene blue = N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + reduced methylene blue
show the reaction diagram
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Enzyme Kinetic Parameters

KM Value (1 result)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.000044
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References & Links

Links to other databases for reduced methylene blue

ChEBI
PubChem
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PubChem