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Ligand 2-nitrophenyl-beta-D-fucopyranoside

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (6 results)

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Show Substrate (6 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.21

2-nitrophenyl-beta-D-fucoside + H2O = 2-nitrophenol + beta-D-fucose

326272

-

3.2.1.21

o-nitrophenyl-beta-D-fucopyranoside + H2O = o-nitrophenol + beta-D-fucopyranose

696166

-

3.2.1.23

2-nitrophenyl-beta-D-fucopyranoside + H2O = 2-nitrophenol + beta-D-fucose

678474

-

3.2.1.23

o-nitrophenyl beta-D-fucopyranoside + H2O = o-nitrophenol + beta-D-fucose

654248

-

3.2.1.23

o-nitrophenyl beta-D-fucoside + H2O = o-nitrophenol + beta-D-fucose

171297, 171326

-

3.2.1.21

2 entries

3.2.1.23

3 entries

3.2.1.B34

2-nitrophenyl beta-D-fucopyranoside + H2O = 2-nitrophenol + D-fucopyranose

723964

-

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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Show Inhibitors (1 entry)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

3.2.1.21

competitively versus substrate 4-nitrophenyl beta-D-glucopyranoside

664149

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (2 results)

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Show Turnover Number (2 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.23

1345

-

pH 6.5, 65°C, wild-type enzyme

678474

3.2.1.B34

26.4

-

pH 5.5, 90°C

723964

K_M Value (3 results)

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Show KM Value (3 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.2.1.23

7.3

-

pH 6.5, 65°C, wild-type enzyme

678474

3.2.1.23

56.9

-

-

171326

3.2.1.23

2 entries

3.2.1.B34

0.116

-

pH 5.5, 90°C

723964

K_i Value (1 result)

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Show KI Value (1 entry)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.2.1.21

0.41

-

pH 7.3, 40°C, versus substrate 4-nitrophenyl beta-D-glucopyranoside

664149

References & Links

Literature References (8)

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Show Reference (8 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

171297

3109378

Physicochemical characterization of human intestinal lactase

1987

Lau, H.K.F.

Biochem. J.

241

567-572

171326

106876

Purification and properties of acid beta-galactosidase from feline liver

1979

Holmes, E.W.; O'Brien, J.S.

Biochemistry

18

952-958

326272

10580648

Cloning and expression of a beta-glycosidase gene from Thermus thermophilus. Sequence and biochemical characterization of the encoded enzyme

1999

Dion, M.; Fourage, L.; Hallet, J.N.; Colas, B.

Glycoconj. J.

16

27-37

654248

10831422

Characterization of a salt-tolerant family 42 beta-galactosidase from a psychrophilic antarctic Planococcus isolate

2000

Sheridan, P.P.; Brenchley, J.E.

Appl. Environ. Microbiol.

66

2438-2444

664149

16417459

Purification and characterization of an intracellular beta-glucosidase from the methylotrophic yeast Pichia pastoris

2005

Turan, Y.; Zheng, M.

Biochemistry (Moscow)

70

1363-1368

678474

18068682

Isolation and characterization of a new family 42 beta-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: identification of the active site residues

2008

Di Lauro, B.; Strazzulli, A.; Perugino, G.; La Cara, F.; Bedini, E.; Corsaro, M.M.; Rossi, M.; Moracci, M.

Biochim. Biophys. Acta

1784

292-301

696166

18774938

A pseudo-beta-glucosidase in Arabidopsis thaliana: correction by site-directed mutagenesis, heterologous expression, purification, and characterization

2008

Turan, Y.

Biochemistry (Moscow)

73

912-919

723964

19626290

Hydrolysis and transglycosylation activity of a thermostable recombinant beta-glycosidase from Sulfolobus acidocaldarius

2010

Park, A.R.; Kim, H.J.; Lee, J.K.; Oh, D.K.

Appl. Biochem. Biotechnol.

160

2236-2247

Links to other databases for 2-nitrophenyl-beta-D-fucopyranoside

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Show Links (1 entry)

ChEBI

PubChem