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Basic Ligand Information
Roles as Enzyme Ligand
Enzyme Kinetic Parameters
References & Links
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Ligand beta-(1->3)-galactobiose

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Basic Ligand Information

Molecular Structure

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Molecular Formula

BRENDA Name

InChIKey

C₁₂H₂₂O₁₁

beta-(1->3)-galactobiose

QIGJYVCQYDKYDW-BAYKCOMVSA-N

Synonyms:

(beta-(1->3)-Gal)2, 3-beta-D-galactosyl-D-galactose, 3-beta-galactosyl-galactose, 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose, 3-O-beta-D-Galactosyl-galactose, beta-(1,3)-galactobiose, beta-(1-3)-D-galactobiose, beta-(1-3)-galactobiose, beta-1,3-galactobiose, beta-1,3-linked galactobiose, beta-D-galactosyl-1,3-beta-D-galactose, beta-galactosido-(1-3)-beta-galactoside, D-galactosyl-beta-1,3-D-galactose, Galbeta(1-3)Gal, Galbeta1-3Gal

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (10 results)

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EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.135

UDPglucuronate + 3-beta-D-galactosyl-D-galactose = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-D-galactose

393084, 393083

2.4.1.135

UDP-Gal + Galbeta(1-3)Gal = Galbeta(1-3)Galbeta(1-3)Gal + UDP

659180

2.4.1.135

UDP-galacturonic acid + Galbeta(1-3)Gal = Galbeta(1-3)Galbeta(1-3)Gal + UDP

659180

2.4.1.135

UDP-GlcNAc + Galbeta(1-3)Gal = GlcNAcbeta(1-3)Galbeta(1-3)Gal + UDP

659180

2.4.1.135

UDP-glucuronate + Galbeta(1-3)Gal = GlcAbeta(1-3)Galbeta(1-3)Gal + UDP

659180

2.4.1.135

UDP-Man + Galbeta(1-3)Gal = Manbeta(1-3)Galbeta(1-3)Gal + UDP

659180

2.4.1.135

UDP-glucuronate + Galbeta1-3Gal = UDP + Galbeta1-3Galbeta1-3Gal

676957

3.2.1.145

beta-(1,3)-galactobiose + H2O = D-galactose

713904

3.2.1.145

beta-(1-3)-galactobiose + H2O = D-galactose

171335, 666634, 677662, 678786

beta-1,3-galactobiose + H2O = beta-D-galactose

171288, 171335

Product in Enzyme-catalyzed Reactions (10 results)

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EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.179

UDP-galactose + beta-D-galactose = UDP + beta-D-galactosyl-1,3-beta-D-galactose

2.4.1.247

D-galactose + alpha-D-galactose 1-phosphate = D-galactosyl-beta-1,3-D-galactose + phosphate

3.2.1.145

beta-1,3-galactotriose + H2O = D-galactose + beta-(1,3)-galactobiose

3.2.1.145

beta-(1-3)-D-galactotriose + H2O = beta-(1-3)-D-galactobiose + D-galactose

3.2.1.145

beta-galactosido-(1-3)-beta-galactosido-(1-6)-galactoside + H2O = beta-galactosido-(1-3)-beta-galactoside + D-galactose

3.2.1.181

beta-1,3-galactotetraose + H2O = 2 beta-1,3-galactobiose

3.2.1.181

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O = 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

3.2.1.181

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O = 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

3.2.1.181

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O = 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + D-galactose

3.2.1.181

beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + H2O = 3-O-beta-D-galactopyranosyl-beta-D-galactopyranose + beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranosyl-(1->3)-beta-D-galactopyranose + D-galactopyranose

Inhibitor in Enzyme-catalyzed Reactions (1 result)

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EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.4.1.135

with 3-galactosyl-4-galactosyl-xylose as substrate

393083

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.2.1.145

28.6

pH 4.0, 37Â°C

666634

K_M Value (6 results)

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EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.4.1.135

0.0091

393084

2.4.1.135

4.48

37Â°C, pH 6.5

676957

3.2.1.145

1.77

pH 4.0, 37Â°C

31.6

7.79

31.6

References & Links

Literature References (10)

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BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

171288

16666809

A beta-galactosidase from radish (Raphanus sativus L.) seeds

1989

Sekimata, M.; Ogura, K.; Tsumuraya, Y.; Hashimoto, Y.; Yamamoto, S.

Plant Physiol.

567-574

171335

2158993

Purification of an exo-beta-(1->3)-D-galactanase of Irpex lacteus (Polyporus tulipiferae) and its action on arabinogalactan-proteins

1990

Tsumuraya, Y.; Mochizuki, N.; Hashimoto, Y.; Kovac, P.

J. Biol. Chem.

265

7207-7215

393083

5770003

Biosynthesis of chondroitin sulfate. II. Glucuronosyl transfer in the formation of the carbohydrate-protein linkage region

1969

Helting, T.; Roden, L.

J. Biol. Chem.

244

2799-2805

393084

4260846

Biosynthesis of heparin. Solubilization and partial purification of uridine diphosphate glucuronic acid: acceptor glucuronosyltransferase from mouse mastocytoma

1972

Helting, T.

J. Biol. Chem.

247

4327-4332

659180

11986319

The donor substrate specificity of the human beta1,3-glucuronosyltransferase I toward UDP-glucuronic acid is determined by two crucial histidine and arginine residues

2002

Ouzzine, M.; Gulberti, S.; Levoin, N.; Netter, P.; Magdalou, J.; Fournel-Gigleux, S.

J. Biol. Chem.

277

25439-25445

666634

15980190

Molecular cloning of a b-galactosidase from radish that specifically hydrolyzes beta-(1->3)- and beta-(1->6)-galactosyl residues of arabinogalactan protein

2005

Kotake, T.; Dina, S.; Konishi, T.; Kaneko, S.; Igarashi, K.; Samejima, M.; Watanabe, Y.; Kimura, K.; Tsumuraya, Y.

Plant Physiol.

138

1563-1576

676957

16300963

Purification and characterization of a soluble form of the recombinant human galactose-beta1,3-glucuronosyltransferase I expressed in the yeast Pichia pastoris

2006

Lattard, V.; Fondeur-Gelinotte, M.; Gulberti, S.; Jacquinet, J.C.; Boudrant, J.; Netter, P.; Magdalou, J.; Ouzzine, M.; Fournel-Gigleux, S.

Protein Expr. Purif.

137-143

677662

16672498

Characterization of an exo-beta -1,3-galactanase from Clostridium thermocellum

2006

Ichinose, H.; Kuno, A.; Kotake, T.; Yoshida, M.; Sakka, K.; Hirabayashi, J.; Tsumuraya, Y.; Kaneko, S.

Appl. Environ. Microbiol.

3515-3523

678786