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Ligand m7GDP

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)

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Show Natural Substrate (1 entry)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.6.1.59

7-methylguanosine 5'-diphosphate + H2O = 7-methylguanosine 5'-phosphate + phosphate

716897, 688336, 716801, 716209

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In Vivo Product in Enzyme-catalyzed Reactions (3 results)

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Show Natural Product (3 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.6.1.62

m7G5'ppp5'-mRNA + H2O = m7GDP + 5'-phospho-mRNA

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-

3.6.1.62

m7G5'ppp5'-U8 snoRNA + H2O = m7GDP + 5'-phospho-U8 snoRNA

-

-

3.6.1.62

a 5'-(N7-methylguanosine 5'-triphospho)-[mRNA] + H2O = N7-methylguanosine 5'-diphosphate + a 5'-phospho-[mRNA]

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-

3.6.1.62

3 entries

Substrate in Enzyme-catalyzed Reactions (5 results)

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Show Substrate (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.7.7.88

5'-triphospho-AACAG + 7-methylguanosine 5'-diphosphate = diphosphate + 7-methylguanosine 5'-triphospho-AACAG

748511

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3.1.3.5

7-methylguanosine diphosphate + H2O = 7-methylguanosine monophosphate + phosphate

750046

-

3.1.3.91

N7-methyl-GDP + H2O = N7-methyl-guanosine + 2 phosphate

725530

-

3.6.1.59

7-methylguanosine 5'-diphosphate + H2O = 7-methylguanosine 5'-phosphate + phosphate

715901, 716801, 716897, 688336, 716209

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3.6.1.59

7-methylguanosine 5'-diphosphate + nucleotidyl 5'-diphosphate + H2O = 7-methylguanosine 5'-phosphate + ?

716209

-

3.6.1.59

2 entries

Product in Enzyme-catalyzed Reactions (20 results)

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Show Product (20 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

2.1.1.56

S-adenosyl-L-methionine + GDP = S-adenosyl-L-homocysteine + m7GDP

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-

3.6.1.29

7-methylguanosine(5')triphospho(5')7-methylguanosine + H2O = 7-methylguanosine(5')monophosphate + 7-methylguanosine(5')diphosphate

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3.6.1.29

7-methylguanosine(5')triphospho(5')adenosine + H2O = 7-methylguanosine(5')monophosphate + ADP + AMP + 7-methylguanosine(5')diphosphate

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-

3.6.1.29

7-methylguanosine(5')triphospho(5')cytosine + H2O = 7-methylguanosine(5')monophosphate + CDP + CMP + 7-methylguanosine(5')diphosphate

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-

3.6.1.29

7-methylguanosine(5')triphospho(5')guanosine + H2O = 7-methylguanosine(5')monophosphate + GDP + 7-methylguanosine(5')diphosphate + GMP

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-

3.6.1.29

7-methylguanosine(5')triphospho(5')uracil + H2O = 7-methylguanosine(5')monophosphate + UDP + UMP + 7-methylguanosine(5')diphosphate

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-

3.6.1.29

m7GpppA + H2O = N7-methyl-GMP + ADP + N7-methyl-GDP + AMP

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-

3.6.1.29

m7Gpppm7G + H2O = N7-methyl-GMP + N7-methyl-GDP

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-

3.6.1.62

5'-(N7-methylguanosine 5'-triphospho)-mRNA + H2O = m7GDP + 5'-phospho-mRNA

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-

3.6.1.62

m7G5'ppp5'-mRNA + H2O = m7GDP + 5'-phospho-mRNA

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-

3.6.1.62

m7G5'ppp5'-NO38 mRNA + H2O = m7GDP + 5'-phospho-NO38 mRNA

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3.6.1.62

m7G5'ppp5'-U3 snoRNA + H2O = m7GDP + 5'-phospho-U3 snoRNA

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3.6.1.62

m7G5'ppp5'-U3 snoRNA + H2O = m7GDP + U3 snoRNA + H2O

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-

3.6.1.62

m7G5'ppp5'-U8 snomRNA + H2O = m7GDP + 5'-phospho-U8 snomRNA

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-

3.6.1.62

m7G5'ppp5'-U8 snoRNA + H2O = m7GDP + 5'-phospho-U8 snoRNA

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-

3.6.1.62

a 5'-(N7-methylguanosine 5'-triphospho)-[mRNA] + H2O = N7-methylguanosine 5'-diphosphate + a 5'-phospho-[mRNA]

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3.6.1.9

7-methyl 5'-guanosine 2'-O-methylgunaosine triphosphate + H2O = 7-methyl-GMP + 7-methyl-GDP + GMP + GDP

246817

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3.6.1.9

P1-5'-(7-methyl)-guanosine-P3-5'-guanosine triphosphate + H2O = 7-methylguanosine 5'-diphosphate + GMP

246801

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3.6.1.29

7 entries

3.6.1.62

8 entries

3.6.1.9

2 entries

3.6.4.13

7-methyl-GTP + H2O = 7-methyl-GDP + phosphate

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5.6.1.6

7-methyl-GTP + H2O + closed Cl- channel = 7-methyl-GDP + phosphate + open Cl- channel

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Inhibitor in Enzyme-catalyzed Reactions (4 results)

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Show Inhibitors (4 entries)

EC NUMBER

COMMENTARY

LITERATURE

ENZYME 3D STRUCTURE

2.7.7.88

-

748511

-

3.6.1.62

although product inhibition by released m7GDP may occur, the amount of m7GDP released from RNA by X29 in these decapping reactions is well below the amount found to inhibit X29 decapping activity in the assay

715488

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3.6.1.62

inhibits decapping at large molar ratios relative to capped RNA substrate

716742

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3.6.1.62

D9 differs from D10 in having lower sensitivity to inhibition by nucleotide cap analogs unattached to RNA

716062

-

3.6.1.62

3 entries

3D Structure of Enzyme-Ligand-Complex (PDB) (36 results)

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Show 3D Structure of Enzyme-Ligand-Complex (PDB) (36 entries)

EC NUMBER

ENZYME 3D STRUCTURE

2.7.7.48

6evj, 3D-view

2.7.7.48

6qcv, 3D-view

2.7.7.48

6qcw, 3D-view

2.7.7.48

6qcx, 3D-view

2.7.7.48

6evj, 3D-view

2.7.7.48

6qcv, 3D-view

2.7.7.48

6qcw, 3D-view

2.7.7.48

6qcx, 3D-view

2.7.7.48

6evj, 3D-view

2.7.7.48

6qcv, 3D-view

2.7.7.48

6qcw, 3D-view

2.7.7.48

6qcx, 3D-view

2.7.7.48

6evj, 3D-view

2.7.7.48

6qcv, 3D-view

2.7.7.48

6qcw, 3D-view

2.7.7.48

6qcx, 3D-view

2.7.7.48

6evj, 3D-view

2.7.7.48

6qcv, 3D-view

2.7.7.48

6qcw, 3D-view

2.7.7.48

6qcx, 3D-view

2.7.7.48

6evj, 3D-view

2.7.7.48

6qcv, 3D-view

2.7.7.48

6qcw, 3D-view

2.7.7.48

6qcx, 3D-view

2.7.7.48

6evj, 3D-view

2.7.7.48

6qcv, 3D-view

2.7.7.48

6qcw, 3D-view

2.7.7.48

6qcx, 3D-view

2.7.7.48

6evj, 3D-view

2.7.7.48

6qcv, 3D-view

2.7.7.48

6qcw, 3D-view

2.7.7.48

6qcx, 3D-view

2.7.7.48

6evj, 3D-view

2.7.7.48

6qcv, 3D-view

2.7.7.48

6qcw, 3D-view

2.7.7.48

6qcx, 3D-view

2.7.7.48

36 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (1 result)

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Show Turnover Number (1 entry)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.6.1.59

0.0017

-

pH 7.0, 30°C, Dcs1 homodimer

688336

K_M Value (1 result)

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Show KM Value (1 entry)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.6.1.59

0.00026

-

pH 7.0, 30°C, Dcs1 homodimer

688336

K_i Value (1 result)

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Show KI Value (1 entry)

EC NUMBER

KI VALUE [MM]

KI VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

2.7.7.88

0.000038

-

at pH 7.5 and 30°C

748511

IC₅₀ Value (2 results)

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Show IC50 Value (2 entries)

EC NUMBER

IC50 VALUE

IC50 VALUE MAXIMUM

COMMENTARY

LITERATURE

2.7.7.88

0.00039

-

at pH 7.5 and 30°C

748511

3.6.1.62

0.3

-

pH 7.5, 37°C

716742

References & Links

Literature References (13)

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Show Reference (13 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

246801

10981813

Purification and properties of a nucleotide pyrophosphatase from lentil seddlings

2000

Medda, R.; Padiglia, A.; Lorrai, A.; Murgia, B.; Agro, A.F.; Castagnola, M.; Floris, G.

J. Protein Chem.

19

209-214

246817

6147251

Nucleotide pyrophosphatase from potato tubers. Purification and properties

1984

Bartkiewicz, M.; Sierakowska, H.; Shugar, D.

Eur. J. Biochem.

143

419-426

688336

16963086

Dcs2, a novel stress-induced modulator of m7GpppX pyrophosphatase activity that locates to P bodies

2006

Malys, N.; McCarthy, J.E.

J. Mol. Biol.

363

370-382

715488

17567574

Metal determines efficiency and substrate specificity of the nuclear NUDIX decapping proteins X29 and H29K (Nudt16)

2007

Peculis, B.A.; Reynolds, K.; Cleland, M.

J. Biol. Chem.

282

24792-24805

715901

15769464

Crystal structures of human DcpS in ligand-free and m7GDP-bound forms suggest a dynamic mechanism for scavenger mRNA decapping

2005

Chen, N.; Walsh, M.A.; Liu, Y.; Parker, R.; Song, H.

J. Mol. Biol.

347

707-718

716062

17881455

Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses

2007

Parrish, S.; Moss, B.

J. Virol.

81

12973-12978

716209

16260594

Scavenger decapping activity facilitates 5' to 3' mRNA decay

2005

Liu, H.; Kiledjian, M.

Mol. Cell. Biol.

25

9764-9772

716742

17283339

Vaccinia virus D10 protein has mRNA decapping activity, providing a mechanism for control of host and viral gene expression

2007

Parrish, S.; Resch, W.; Moss, B.

Proc. Natl. Acad. Sci. USA

104

2139-2144

716801

14523240

DcpS can act in the 5'-3' mRNA decay pathway in addition to the 3'-5' pathway

2003

van Dijk, E.; Le Hir, H.; Séraphin, B.

Proc. Natl. Acad. Sci. USA

100

12081-12086

716897

18426921

DcpS scavenger decapping enzyme can modulate pre-mRNA splicing

2008

Shen, V.; Liu, H.; Liu, S.W.; Jiao, X.; Kiledjian, M.

RNA

14

1132-1142

725530

23223233

Identification of Drosophila and human 7-methyl GMP-specific nucleotidases

2013

Buschmann, J.; Moritz, B.; Jeske, M.; Lilie, H.; Schierhorn, A.; Wahle, E.

J. Biol. Chem.

288

2441-2451

748511

28053102

5'-Phospho-RNA acceptor specificity of GDP polyribonucleotidyltransferase of vesicular stomatitis virus in mRNA capping

2017

Ogino, M.; Ogino, T.

J. Virol.

91

e02322-16

750046

29195795

7-Methylguanosine monophosphate analogues with 5-(1,2,3-triazoyl) moiety Synthesis and evaluation as the inhibitors of cNIIIB nucleotidase

2018

Kozarski, M.; Kubacka, D.; Wojtczak, B.A.; Kasprzyk, R.; Baranowski, M.R.; Kowalska, J.

Bioorg. Med. Chem.

26

191-199

Links to other databases for m7GDP

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Show Links (1 entry)

ChEBI

PubChem

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