Ligand 3-methylthio-2-oxobutanoate
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Basic Ligand Information
Molecular Structure

C5H8O3S
3-methylthio-2-oxobutanoate
IBAVCTDEMUWOLM-UHFFFAOYSA-M
2-oxo-3-methiobutyrate, 3-(methylthio)-2-oxobutanoate, 3-Methylthio-2-ketobutyrate, 3-Methylthio-2-oxobutyrate
Roles as Enzyme Ligand
In Vivo Product in Enzyme-catalyzed Reactions (1 result)
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1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 3-(methylthio)-2-oxobutanoate + formate
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Substrate in Enzyme-catalyzed Reactions (2 results)
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L-leucine + 2-oxo-3-methiobutyrate = 2-oxoisohexanoate + L-methionine
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L-isoleucine + 3-methylthio-2-oxobutanoate = 3-methyl-2-oxopentanoate + 2-amino-3-methylthiobutanoate
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Product in Enzyme-catalyzed Reactions (2 results)
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1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 3-(methylthio)-2-oxobutanoate + formate
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L-Met + H2O + NAD+ = 3-methylthio-2-oxobutanoate + NH3 + NADH
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Enzyme Kinetic Parameters
KM Value (1 result)
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References & Links
Literature References (4)
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L-Leucine dehydrogenase from Bacillus cereus
1985
Schuette, H.; Hummel, W.; Tsai, H.; Kula, M.R.
Appl. Microbiol. Biotechnol.
22
306-317
Isolation and characterization of leucine dehydrogenase from Bacillus subtilis
1988
Livesey, G.; Lund, P.
Methods Enzymol.
166
282-288
Transamination in Escherichia coli
1953
Rudman, D.; Meister, A.
J. Biol. Chem.
200
591-604
Branched chain aminotransferase isoenzymes. Purification and characterization of the rat brain isoenzyme
1993
Hall, T.R.; Wallin, R.; Reinhart, G.D.; Hutson, S.M.
J. Biol. Chem.
268
3092-3098
Links to other databases for 3-methylthio-2-oxobutanoate