Ligand GFA
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Basic Ligand Information
Molecular Structure

C14H19N3O4
GFA
WMGHDYWNHNLGBV-ONGXEEELSA-N
Gly-Phe-Ala, H-Gly-Phe-Ala-OH
Roles as Enzyme Ligand
Substrate in Enzyme-catalyzed Reactions (3 results)
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Gly-Phe-Ala + H2O = Gly + Phe-Ala
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GFA + H2O = Phe-Ala + Gly
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H-Gly-Phe-Ala-OH + H2O = ?
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Product in Enzyme-catalyzed Reactions (1 result)
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Gly-Gly-Phe-Ala + H2O = Gly + Gly-Phe-Ala
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Enzyme Kinetic Parameters
kcat Value (Turnover Number) (2 results)
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0.076
-
pH 8.0, 37°C, 5 M glycerol
KM Value (2 results)
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0.6
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pH 8.0, 37°C, 5 M glycerol
References & Links
Literature References (4)
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An extracellular aminopeptidase from Clostridium histolyticum
1976
Kessler, E.; Yaron, A.
Eur. J. Biochem.
63
271-287
Extracellular aminopeptidase from Clostridium histolyticum
1976
Kessler, E.; Yaron, A.
Methods Enzymol.
45
544-552
A novel aminopeptidase from Clostridium histolyticum
1973
Kessler, E.; Yaron, A.
Biochem. Biophys. Res. Commun.
50
405-412
Additional data about thermolysin specificity in buffer- and glycerol-containing media
1997
Ligne, T.; Pauthe, E.; Monti, J.P.; Gacel, G.; Larreta-Garde, V.
Biochim. Biophys. Acta
1337
143-148
Links to other databases for GFA