Ligand birchwood xylan

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Basic Ligand Information

Molecular Formula
BRENDA Name
InChIKey
birchwood xylan

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
birchwood xylan + H2O = ferulic acid + ?
-
show the reaction diagram
birchwood xylan + H2O = L-arabinose + ?
-

Substrate in Enzyme-catalyzed Reactions (41 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
birchwood xylan + H2O = ?
-
show the reaction diagram
birchwood xylan + H2O = ?
-
show the reaction diagram
birchwood xylan + H2O = ferulic acid + ?
-
show the reaction diagram
birchwood xylan + H2O = ?
-
show the reaction diagram
birchwood xylan + H2O = ?
-
show the reaction diagram
birchwood xylan + H2O = ?
-

Product in Enzyme-catalyzed Reactions (1 result)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
acetylated birchwood xylan + H2O = acetate + birchwood xylan
-

Inhibitor in Enzyme-catalyzed Reactions (1 result)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
mixed inhibition
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (52 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
wild-type, pH 6.5, 40°C
289
-
mutant W773A, pH 6.5, 40°C
129
-
mutant W689A, pH 6.5, 40°C
116
-
mutant R331A, pH 6.5, 40°C
0.008
-
in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C
11.1
-
mutant F696A, pH 6.5, 40°C
86
-
mutant E381A, pH 6.5, 40°C
0.3
-
mutant D215A, pH 6.5, 40°C
1
-
mutant R331A, pH 6.5, 40°C
0.008
-
wild-type, pH 6.5, 40°C
289
-
recombinant wild-type enzyme, pH 6.5, 37°C, GH30 glucuronoxylan-specific xylanase treated substrate
mutant W773A, pH 6.5, 40°C
129
-
mutant W689A, pH 6.5, 40°C
116
-
mutant F696A, pH 6.5, 40°C
86
-
mutant E381A, pH 6.5, 40°C
0.3
-
mutant D215A, pH 6.5, 40°C
1
-
at pH 4.0 and 50°C
19.4
-
pH 5.7, 50°C, recombinant catalytic module CtGH43
0.48
-
pH 5.7, 50°C, recombinant full-length enzyme
0.45
-
recombinant enzyme, pH 5.0, 55°C
245
-
pH 5.5, 85°C
465
-
pH 6.0, 50°C
125.9
-
pH 6.0, 65°C
222
-
pH 6.5, 50°C
4.26
-
pH 7.5, 50°C
1.7
-
mutant Y174W
21
-
apparent value for the recombinant enzyme
292
-
apparent value for the native enzyme
872
-
wild type enzyme, at pH 7.0
wild-type
apparent value for the native enzyme
277
-
-
1737
-
in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C
72.07
-
mutant D11F/R122D
39
-
Km value is 2.95 mg/ml, pH 5.0, 50°C
7764
-
isozyme IIc, pH 6.0, 70°C
10.5
-
isozyme IIa, pH 6.0, 70°C
73
-
mutant G12W
65.4
-
mutant N35D
50.7
-
mutant Q127K
14.6
-
mutant R112Y
8.8
-
mutant Y174W
111.2
-
-
103.4
-
in 48.5 mM citric acid and 103 mM disodium hydrogen phosphate, pH 5, at 40°C
2
-
His-tagged enzyme, pH 6.0, 40°C
36.8
-
apparent value for the recombinant enzyme
997
-
mutant W9Y
88.7
-
mutant W9Y/N35D

KM Value (26 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
pH 8.2, 58°C
16.72
-
km value of wild-type 43 mg/ml, pH 6.5, 40°C
-999
-
in 50 mM sodium phosphate, 12 mM citrate buffer, pH 6.5, at 37°C
0.3
-
recombinant wild-type enzyme, pH 6.5, 37°C, GH30 glucuronoxylan-specific xylanase treated substrate
km vaule of wild-type 43 mg/ml, pH 6.5, 40°C
-999
-
Km value 4.37 mg/ml, pH 5.0, temperature not specified in the publication
-999
-
pH 5.7, 50°C, recombinant full-length enzyme
0.95
-
pH 5.7, 50°C, recombinant catalytic module CtGH43
0.9
-
Km value 7.1 mg/ml, pH 6.5, 50°C
-999
-
truncated variant XynAGN16L, Km value 2.6 mg/ml, truncated variant 1.2 mg/ml, respcetively, pH 6.5, 37°C
-999
-
pH 6.0, 95°C, Km-value: 3.5 mg/ml
-999
-
Km value is 4.4 mg/ml, pH 11, 70°C
-999
-
Km value is 28.3 mg/ml, pH 6.5, 65°C
-999
-
Km value is 2.95 mg/ml, pH 5.0, 50°C
-999
-
Km value 4.39 mg/ml, pH 6.0, 50°C
-999
-
Km value 3.26 mg/ml, pH 6.5, 50°C
-999
-
Km value 3.0 mg/ml, pH 6.5, 65°C
-999
-
Km value 22.5 mg/ml, pH 7.0, 50°C
-999
-
Km value 11.7 mg/ml, pH 5.0, 50°C
-999
-
Km value 10.5 mg/ml, pH 6.5, 80°C
-999
-
Km value 1.5 mg/ml, pH 6.0, 65°C
-999
-
Km value 1.44 mg/ml, pH 5.0, 50°C
-999
-
Km value 1.05 mg/ml, pH 6.5, 65°C
-999
-
Km value 0.9 mg/ml, pH 7, 65°C
-999
-
Km value 0.55 mg/ml, pH 5.5, 85°C
-999
-

Ki Value (1 result)

COMMENTARY
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
LITERATURE
Kic 0.093 g/l, Kiu 0.18 g/l, pH 5.0, 37°C
-999
-

References & Links

Links to other databases for birchwood xylan

ChEBI
PubChem
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