Ligand vitamin D3

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Basic Ligand Information

Molecular Structure
Picture of vitamin D3 (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C27H44O
vitamin D3
Synonyms:
calciol, cholecalciferol, vitamin D


Show all pahtways known for Show all BRENDA pathways known for vitamin D3

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
calciol + O2 + [reduced NADPH-hemoprotein reductase] = calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
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show the reaction diagram
cholecalciferol + NADPH + O2 = 25-hydroxyvitamin D3 + NADP+ + H2O
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Substrate in Enzyme-catalyzed Reactions (13 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
cholecalciferol + H2O2 = 25-hydroxycholecalciferol + 24-hydroxycholecalciferol + 26,27-hydroxycholecalciferol + H2O
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show the reaction diagram
cholecalciferol + NADPH + O2 = 25-hydroxyvitamin D3 + NADP+ + H2O
-
show the reaction diagram
vitamin D3 + reduced adrenodoxin + O2 = 20-hydroxyvitamin D3 + oxidized adrenodoxin + H2O
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Activator in Enzyme-catalyzed Reactions (4 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE

Inhibitor in Enzyme-catalyzed Reactions (6 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
equimolar to substrate inhibits 38% of enzyme activity
-
inhibits reductive activity in HCT8 and LoVo cells
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decreases parathyroid-induced stimulation of AC6 and induces a phosphorylation of AC6 in a PKC-dependent manner
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22% inhibition at 0.00001 mM, reduces dexamethasone induced increase in enzyme expression
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (8 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
pH 7.4, 37°C
0.008
-
pH 7.7, 37°C
25-hydroxylation, mutant R73V/R84A, pH not specified in the publication, temperature not specified in the publication
0.0023
-
in the presence of 0.45% cyclodextrin
0.328
-
in the presence of 4.5% cyclodextrin
1.6
-
isoenzyme S25DH1, pH 7.0, 30°C
0.23
-

KM Value (12 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
30°C, pH not specified in the publication
0.0135
-
mitochondrial enzyme, pH not specified in the publication, temperature not specified in the publication
0.02
-
mutant T70R/V156L/E216M/E384R, 30°C, pH not specified in the publication
0.032
-
pH 7.4, 37°C
0.0044
-
pH 7.7, 37°C
25-hydroxylation, mutant R73V/R84A, pH not specified in the publication, temperature not specified in the publication
0.0035
-
vitamin D3 25-hydroxylation, pH 7.4, 30°C
0.00054
-
in the presence of 0.45% cyclodextrin
0.0296
-
in the presence of 4.5% cyclodextrin
3.67
-
isoenzyme S25DH1, pH 7.0, 30°C
0.29
-
pH 7.5, 37°C
0.0000145
-

Ki Value (1 result)

COMMENTARY
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
LITERATURE
-
0.001
0.002

References & Links

Links to other databases for vitamin D3

ChEBI
PubChem
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