Ligand vitamin D3

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

Basic Ligand Information

Molecular Structure
Picture of vitamin D3 (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C27H44O
vitamin D3
Synonyms:
calciol, cholecalciferol, vitamin D
Pathway Source
Pathways
BRENDA
vitamin D3 metabolism
KEGG
Biosynthesis of secondary metabolites, Rheumatoid arthritis, Steroid biosynthesis, Vitamin digestion and absorption
MetaCyc
vitamin D3 biosynthesis


Show all pahtways known for Show all BRENDA pathways known for vitamin D3

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (4 results)

top print hide
EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
calciol + O2 + [reduced NADPH-hemoprotein reductase] = calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
show the reaction diagram
cholecalciferol + NADPH + O2 = 25-hydroxyvitamin D3 + NADP+ + H2O
-

Substrate in Enzyme-catalyzed Reactions (13 results)

top print hide
EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
cholecalciferol + H2O2 = 25-hydroxycholecalciferol + 24-hydroxycholecalciferol + 26,27-hydroxycholecalciferol + H2O
-
show the reaction diagram
cholecalciferol + NADPH + O2 = 25-hydroxyvitamin D3 + NADP+ + H2O
-
show the reaction diagram
vitamin D3 + reduced adrenodoxin + O2 = 20-hydroxyvitamin D3 + oxidized adrenodoxin + H2O
-

Activator in Enzyme-catalyzed Reactions (4 results)

top print hide
COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE

Inhibitor in Enzyme-catalyzed Reactions (6 results)

top print hide
COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
equimolar to substrate inhibits 38% of enzyme activity
-
inhibits reductive activity in HCT8 and LoVo cells
-
decreases parathyroid-induced stimulation of AC6 and induces a phosphorylation of AC6 in a PKC-dependent manner
-
22% inhibition at 0.00001 mM, reduces dexamethasone induced increase in enzyme expression
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (8 results)

top print hide
COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
pH 7.4, 37°C
0.008
-
pH 7.7, 37°C
25-hydroxylation, mutant R73V/R84A, pH not specified in the publication, temperature not specified in the publication
0.0023
-
in the presence of 0.45% cyclodextrin
0.328
-
in the presence of 4.5% cyclodextrin
1.6
-
isoenzyme S25DH1, pH 7.0, 30°C
0.23
-

KM Value (12 results)

top print hide
COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
30°C, pH not specified in the publication
0.0135
-
mitochondrial enzyme, pH not specified in the publication, temperature not specified in the publication
0.02
-
mutant T70R/V156L/E216M/E384R, 30°C, pH not specified in the publication
0.032
-
pH 7.4, 37°C
0.0044
-
pH 7.7, 37°C
25-hydroxylation, mutant R73V/R84A, pH not specified in the publication, temperature not specified in the publication
0.0035
-
vitamin D3 25-hydroxylation, pH 7.4, 30°C
0.00054
-
in the presence of 0.45% cyclodextrin
0.0296
-
in the presence of 4.5% cyclodextrin
3.67
-
isoenzyme S25DH1, pH 7.0, 30°C
0.29
-
pH 7.5, 37°C
0.0000145
-

Ki Value (1 result)

top print hide
COMMENTARY
EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
LITERATURE
-
0.001
0.002

References & Links

top

Links to other databases for vitamin D3

top print hide
ChEBI
PubChem
-
-