Ligand Inosine

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Basic Ligand Information

Molecular Structure
Picture of Inosine (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C10H12N4O5
Inosine
UGQMRVRMYYASKQ-KQYNXXCUSA-N
Synonyms:
9-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl]-1,9-dihydro-6H-purin-6-one, hypoxanthine ribose


Show all pahtways known for Show all BRENDA pathways known for Inosine

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (22 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
inosine + O2 = 9-riburonosylhypoxanthine + H2O
show the reaction diagram
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inosine + 2 O2 + H2O = 9-riburonosylinosine + 2 H2O2
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inosine + phosphate = hypoxanthine + D-ribose 1-phosphate
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ATP + inosine = ADP + IMP
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inosine + H2O = D-ribose + hypoxanthine
show the reaction diagram
inosine + H2O = hypoxanthine + D-ribose
show the reaction diagram
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In Vivo Product in Enzyme-catalyzed Reactions (14 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
IMP + H2O = inosine + phosphate
show the reaction diagram
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S-inosyl-L-homocysteine + H2O = inosine + L-homocysteine
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S-inosyl-L-homocysteine + H2O = L-homocysteine + inosine
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adenosine + H2O = inosine + NH3
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adenosine + H2O = inosine + NH3
show the reaction diagram
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Substrate in Enzyme-catalyzed Reactions (93 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
inosine + NAD+ + H2O = xanthosine + NADH
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inosine + 2 O2 + H2O = 9-riburonosylinosine + 2 H2O2
show the reaction diagram
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inosine + NAD+ + H2O = ? + NADH
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inosine + phosphate = hypoxanthine + D-ribose 1-phosphate
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inosine + phosphate = hypoxanthine + alpha-D-ribose 1-phosphate
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inosine + adenine = adenosine + hypoxanthine
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ATP + inosine = ADP + inosine 5'-phosphate
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ATP + inosine = ADP + inosine 5'-phosphate
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phosphate + inosine = ?
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3 inosine = ?
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inosine + L-homocysteine = S-inosyl-L-homocysteine + H2O
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inosine + H2O = hypoxanthine + D-ribose
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inosine + homocysteine = ?
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L-homocysteine + inosine = S-inosyl-L-homocysteine + H2O
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Product in Enzyme-catalyzed Reactions (50 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
guanosine + hypoxanthine = guanine + inosine
show the reaction diagram
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5'-IMP + H2O = inosine + phosphate
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5'-IMP + H2O = inosine + phosphate
show the reaction diagram
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3'-IMP + H2O = inosine + phosphate
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IMP + H2O = inosine + phosphate
show the reaction diagram
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S-inosyl-L-homocysteine + H2O = inosine + L-homocysteine
show the reaction diagram
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S-inosyl-L-homocysteine + H2O = L-homocysteine + inosine
show the reaction diagram
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adenosine + H2O = inosine + NH3
show the reaction diagram
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adenosine + H2O = inosine + NH3
show the reaction diagram
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adenosine + H2O = inosine + NH3
show the reaction diagram
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adenosine + H2O = inosine + NH3
show the reaction diagram
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adenosine + H2O = inosine + NH3
show the reaction diagram
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Activator in Enzyme-catalyzed Reactions (23 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
0.1 mM, increases activity 5fold
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Inhibitor in Enzyme-catalyzed Reactions (47 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
very weak, non-competitive
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1 mM, about 25% inhibition
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weak, competitive
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slight inhibition
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inhibits phosphorylation of guanosine
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concentrations above 0.1 mM lead to reduction in enzyme activity
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strong inhibition, enzymes N-I and N-II
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above 2 mM
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competitive inhibitor of xanthosine hydrolysis
noncompetitive
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at 0.2 mM: 8.5% inhibition for form Lf, 10.5% inhibition for form R2, form R1 not inhibited
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at 6.25 mM, competitive inhibitor for uridine hydrolysis
competitive inhibition of deamination of adenosine 3'-phenylphosphonate
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inhibits independently of the A3 receptor
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3D Structure of Enzyme-Ligand-Complex (PDB) (32 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (160 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.57
-
pH 7.4, 25°C
0.16
-
pH 8.0, 22°C
0.83
-
pH 7.0, 70°C
0.83
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pH 7.5, 70°C, recombinant enzyme

KM Value (236 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.044
-
-
0.072
-
-
0.005
-
pH 7.5, 25°C
0.09
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pH 7.4, 25°C
0.64
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pH 7.0, 70°C
0.283
-
pH 7.2, 32°C
2
-
-
0.64
-
pH 7.5, 70°C, recombinant enzyme

Ki Value (6 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.26
-
-
2
-
pH 7.0, 70°C
3
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pH 7.5, 37°C, wild-type enzyme, versus uridine
0.35
-
pH 7.5, 25°C

IC50 Value (2 results)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE

References & Links

Links to other databases for Inosine

ChEBI
PubChem
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PubChem