Ligand p-nitrophenyl-beta-D-mannopyranoside

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Basic Ligand Information

Molecular Structure
Picture of p-nitrophenyl-beta-D-mannopyranoside (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C12H15NO8
p-nitrophenyl-beta-D-mannopyranoside
IFBHRQDFSNCLOZ-LDMBFOFVSA-N
Synonyms:
4-nitrophenol beta-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranose, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-beta-D-rhamnopyranoside, 4-nitrophenyl-beta-mannopyranose, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl beta-D-mannoside, p-nitrophenol-beta-D-mannopyranoside, p-nitrophenol-beta-D-mannoside, p-nitrophenyl-beta-D-mannoside, p-nitrophenyl-beta-mannoside, p-nitrophenyl beta-D-mannopyranoside

Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (37 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
UDP-Gal + 4-nitrophenyl beta-D-mannopyranoside = UDP + Gal-beta-1,4-Man-pNP
show the reaction diagram
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4-nitrophenyl beta-D-mannopyranoside + H2O = beta-D-mannopyranose + 4-nitrophenol
show the reaction diagram
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4-nitrophenyl beta-D-mannopyranoside + H2O = 4-nitrophenol + beta-D-mannopyranoside
show the reaction diagram
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p-nitrophenyl-beta-D-mannopyranoside + H2O = D-mannopyranose + p-nitrophenol
show the reaction diagram
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Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-peptide + p-nitrophenyl-beta-D-mannoside = Man-beta-(1-4)GlcNAc-beta-1-p-nitrophenyl-beta-D-mannoside + GlcNAc-peptide
show the reaction diagram
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4-nitrophenyl beta-D-mannopyranoside + H2O = 4-nitrophenol + beta-D-mannopyranose
show the reaction diagram
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4-nitrophenyl beta-D-mannopyranoside + H2O = 4-nitrophenol + beta-D-mannose
show the reaction diagram
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4-nitrophenyl beta-D-mannopyranoside + H2O = 4-nitrophenol + beta-D-mannopyranose
show the reaction diagram
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4-nitrophenyl beta-D-mannopyranoside + H2O = 4-nitrophenol + beta-D-mannopyranose
show the reaction diagram
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4-nitrophenyl beta-D-mannopyranoside + H2O = 4-nitrophenol + D-mannopyranose
show the reaction diagram
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transferrin + 4-nitrophenyl-beta-D-mannoside = ?
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4-nitrophenyl beta-D-mannoside + H2O = 4-nitrophenol + D-mannose
show the reaction diagram
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4-nitrophenyl beta-D-mannopyranoside + H2O = 4-nitrophenol + D-mannopyranose
show the reaction diagram
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4-nitrophenyl beta-D-mannopyranoside + H2O = 4-nitrophenol + beta-D-mannopyranose
show the reaction diagram
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4-nitrophenyl beta-D-mannopyranoside + H2O = 4-nitrophenol + D-mannose
show the reaction diagram
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Inhibitor in Enzyme-catalyzed Reactions (3 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
94% inhibition at 3 mM
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above 0.4 mM
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (46 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.032
-
-
2.65
-
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
2
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pH 6.0, 90°C

KM Value (70 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.769
-
pH 5.5, 24°C
24.2
-
pH 6.5 (50 mM phosphate), 45°C, wild-type enzyme
0.14
-
pH 6.0, 90°C

References & Links

Links to other databases for p-nitrophenyl-beta-D-mannopyranoside

ChEBI
PubChem
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PubChem