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Ligand Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester

Basic Ligand Information

Molecular Structure

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Roles as Enzyme Ligand

Substrate in Enzyme-catalyzed Reactions (6 results)

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Show Substrate (6 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

3.4.23.20

H-Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester + H2O = Nle-Ala-Leu methyl ester + Leu-Ser-(4-nitro)Phe

30603

-

3.4.23.20

Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester + H2O = ?

30599

-

3.4.23.20

2 entries

3.4.23.3

Leu-Ser-(4-nitro)Phe-norleucine-Ala-Leu methyl ester + H2O = ?

36853

-

3.4.23.39

Leu-Ser-Phe(NO2)-Nle-Ala-Leu-OMe + H2O = ?

696871

-

3.4.23.4

Leu-Ser-Phe(NO2)-Nle-Ala-Leu-OMe + H2O = ?

36880, 36892

-

3.4.23.40

Leu-Ser-Phe(NO2)-Ahx-Ala-Leu-OMe + H2O = ?

733764

-

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (5 results)

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Show Turnover Number (5 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

3.4.23.20

6.4

-

pH 3.5

30599

3.4.23.20

11.4

-

pH 5.5

30599

3.4.23.20

2 entries

3.4.23.39

10.4

-

pH 4.4, 37°C

696871

3.4.23.40

13.7

-

pH 4.7, 37°C

733764

3.4.23.40

86.2

-

pH 4.7, 37°C

733764

3.4.23.40

2 entries

K_M Value (7 results)

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Show KM Value (7 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

3.4.23.20

0.004

-

pH 5.5

30599

3.4.23.20

0.01

-

pH 3.5

30599

3.4.23.20

2 entries

3.4.23.39

0.0274

-

pH 4.4, 37°C

696871

3.4.23.4

0.333

-

point mutant G243D

36880

3.4.23.4

1.11

-

point mutant A115T

36880

3.4.23.40

0.081

-

pH 4.7, 37°C

733764

3.4.23.40

0.64

-

pH 4.7, 37°C

733764

3.4.23.4

2 entries

3.4.23.40

2 entries

References & Links

Literature References (7)

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Show Reference (7 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

30599

6424704

Effect of pH on the activities of penicillopepsin and Rhizopus pepsin and a proposal for the productive substrate binding mode in penicillopepsin

1984

Hofmann, T.; Hodges, R.S.; James, M.N.G.

Biochemistry

23

635-643

30603

393302

Further characterization of the Penicillium roqueforti acid protease

1979

Houmard, J.; Raymond, M.N.

Biochimie

61

979-982

36853

6800788

Purification and characterization of bovine gastricsin

1982

Martin, P.; Trieu-Cuot, P.; Collin, J.C.; Ribadeau Dumas, B.

Eur. J. Biochem.

122

31-39

36880

9464563

Mutagenesis, biochemical characterization and X-ray structural analysis of point mutants of bovine chymosin

1997

Williams, M.G.; Wilsher, J.; Nugent, P.; Mills, A.; Dhanaraj, V.; Fabry, M.; Sedlacek, J.; Uusitalo, J.M.; Penttila, M.E.; Pitts, J.E.; Blundell, T.L.

Protein Eng.

10

991-997

36892

8931128

Protein engineering loops in aspartic proteinases: site-directed mutagenesis, biochemical characterization and X-ray analysis of chymosin with a replaced loop from rhizopuspepsin

1996

Nugent, P.G.; Albert, A.; Orprayoon, P.; Wilsher, J.; Pitts, J.E.; Blundell, T.L.; Dhanaraj, V.

Protein Eng.

9

885-893

696871

18471091

Generation of an affinity matrix useful in the purification of natural inhibitors of plasmepsin II, an antimalarial-drug target

2009

Ramirez, A.R.; Guerra, Y.; Otero, A.; Garcia, B.; Berry, C.; Mendiola, J.; Hernandez-Zanui, A.; Chavez, M.

Biotechnol. Appl. Biochem.

52

149-157

733764

8654427

Purification, characterization and partial amino acid sequencing of two new aspartic proteinases from fresh flowers of Cynara cardunculus L.

1996

Verissimo, P.; Faro, C.; Moir, A.J.; Lin, Y.; Tang, J.; Pires, E.

Eur. J. Biochem.

235

762-768

Links to other databases for Leu-Ser-(4-nitro)Phe-Nle-Ala-Leu methyl ester

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Show Links (1 entry)

ChEBI

PubChem

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