Ligand 25-hydroxyvitamin D3

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Basic Ligand Information

Molecular Structure
Picture of 25-hydroxyvitamin D3 (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C27H44O2
25-hydroxyvitamin D3
JWUBBDSIWDLEOM-DTOXIADCSA-N
Synonyms:
25-hydroxy-vitamin D3, 25-hydroxy-[3alpha-3H]vitamin D3, 25-hydroxy-[26,27-3H]vitamin D3, 25-hydroxyvitamin D, calcidiol


Show all pahtways known for Show all BRENDA pathways known for 25-hydroxyvitamin D3

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE

In Vivo Product in Enzyme-catalyzed Reactions (4 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
calciol + O2 + [reduced NADPH-hemoprotein reductase] = calcidiol + [oxidized NADPH-hemoprotein reductase] + H2O
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-
show the reaction diagram
vitamin D3 + acceptor + H2O = 25-hydroxy-vitamin D3 + reduced acceptor
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Substrate in Enzyme-catalyzed Reactions (17 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
25-hydroxyvitamin D3 + O2 + NADPH + H+ = 1alpha,25-dihydroxyitamin D3 + NADP+ + H2O
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Product in Enzyme-catalyzed Reactions (8 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
vitamin D3 + acceptor + H2O = 25-hydroxy-vitamin D3 + reduced acceptor
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Inhibitor in Enzyme-catalyzed Reactions (2 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
CYP27B1 shows inhibition when substrate concentrations in the membrane are greater than 4 times Km, more pronounced with 25-hydroxyvitamin D3 than with 25-hydroxyvitamin D2
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Enzyme Kinetic Parameters

kcat Value (Turnover Number) (9 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
in 20 mM Tris (pH 7.5), at 37°C
0.002
-
pH 7.4, 37°C, Michaelis-Menten kinetic parameters
0.68
-
pH 7.4, 37°C, parameters using a kinetic model with substrate binding at two inhibitory sites
0.92
-
pH 7.4, 37°C, recombinant mutant S408A
0.006
-
pH 7.4, 37°C, recombinant mutant S408I
0.0008
-
pH 7.4, 37°C, recombinant mutant S408T
0.098
-
pH 7.4, 37°C, recombinant mutant S408V
0.013
-
pH 7.4, 37°C, recombinant wild-type enzyme
0.385
-
1alpha-hydroxylation, mutant R73V/R84A, pH not specified in the publication, temperature not specified in the publication
0.0023
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KM Value (12 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
30°C, pH not specified in the publication
0.0071
-
in 0.1 mM Tris-HCl (pH 7.4), temperature not specified in the publication
0.00018
-
in 20 mM Tris (pH 7.5), at 37°C
0.00033
-
pH 7.4, 37°C, Michaelis-Menten kinetic parameters
5.9
-
pH 7.4, 37°C, parameters using a kinetic model with substrate binding at two inhibitory sites
9.7
-
pH 7.4, 37°C, recombinant mutant S408A
0.00024
-
pH 7.4, 37°C, recombinant mutant S408I
0.00018
-
pH 7.4, 37°C, recombinant mutant S408T
0.00013
-
pH 7.4, 37°C, recombinant mutant S408V
0.00054
-
pH 7.4, 37°C, recombinant wild-type enzyme
0.00028
-
1alpha-hydroxylation, mutant R73V/R84A, pH not specified in the publication, temperature not specified in the publication
0.0022
-
25-hydroxyvitamin D3 1alpha-hydroxylation, pH 7.4, 30°C
0.00091
-

References & Links

Links to other databases for 25-hydroxyvitamin D3

ChEBI
PubChem
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PubChem