Ligand tRNAMet

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Basic Ligand Information

Molecular Structure
Picture of tRNAMet (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
C28H37N11O21P3R
tRNAMet
Synonyms:
5'-dephospho-RNA (n-1 nucleotides), ribosomal RNA, rRNA, t-RNALeu, tRNA(Ala), tRNA(Arg), tRNA(Asn), tRNA(Asp), tRNA(Cys), tRNA(Gln), tRNA(Glu), tRNA(Gly), tRNA(His), tRNA(Ile), tRNA(Leu), tRNA(Lys), tRNA(lys)), tRNA(Met), tRNA(Phe), tRNA(Pro), tRNA(Ser), tRNA(Thr), tRNA(Trp), tRNA(Tyr), tRNA(Val), tRNA-CCA-3', tRNA-Gly, tRNA-His, tRNA-Leu, tRNA-Met, tRNA-N, tRNA-Phe, tRNA-Tyr, tRNA-Val, tRNA1Glu, tRNA1Gly, tRNA2Glu, tRNA2Gly, tRNAAla, tRNAArg, tRNAAsn, tRNAAsp, tRNAAsx, tRNACys, tRNAGln, tRNAGlu, tRNAGlx, tRNAGly, tRNAguanine, tRNAHis, tRNAHisII, tRNAIle, tRNALys, tRNAPhe, tRNAPro, tRNAPyl, tRNAqueuine, tRNA queuine, tRNASer, tRNAThr, tRNATrp, tRNATyr, tRNAVal


Show all pahtways known for Show all BRENDA pathways known for tRNAMet

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (82 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu = L-glutamyl-tRNAGlu + NADPH + H+
-
show the reaction diagram
tRNAHis + S-adenosyl-L-methionine = methylated tRNAHis + S-adenosyl-L-homocysteine
-
show the reaction diagram
rRNA + H2O = ?
-
show the reaction diagram
ATP + L-cysteine + tRNACys = AMP + diphosphate + L-cysteinyl-tRNACys
-
show the reaction diagram
ATP + L-asparagine + tRNAAsn = AMP + diphosphate + L-asparaginyl-tRNAAsn
-
show the reaction diagram
ATP + L-pyrrolysine + tRNAPyl = AMP + diphosphate + L-pyrrolysyl-tRNAPyl
-
show the reaction diagram
ATP + L-leucine + tRNAIle = AMP + diphosphate + L-leucyl-tRNALeu
-
show the reaction diagram
ATP + L-isoleucine + tRNAIle = AMP + diphosphate + L-isoleucyl-tRNAIle
-
show the reaction diagram
ATP + L-valine + tRNAVal = AMP + diphosphate + L-valyl-tRNAVal
-
show the reaction diagram
ATP + L-glutamate + tRNAAsp = AMP + diphosphate + L-glutamyl-tRNAAsp
-

In Vivo Product in Enzyme-catalyzed Reactions (58 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
PROVEN IN VIVO REACTION
ENZYME 3D STRUCTURE
-
show the reaction diagram
L-glutamyl-tRNAGlu + NADPH + H+ = L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
show the reaction diagram
L-alanyl-tRNAAla + phosphatidylglycerol = tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
show the reaction diagram
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + glycyl-tRNAGly = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-penta-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + tRNAGly
-

Substrate in Enzyme-catalyzed Reactions (321 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu = L-glutamyl-tRNAGlu + NADPH + H+
-
show the reaction diagram
tRNAHis + S-adenosyl-L-methionine = methylated tRNAHis + S-adenosyl-L-homocysteine
-
show the reaction diagram
tRNAguanine + 7-cyano-7-deazaguanine = ?
-
show the reaction diagram
S-adenosyl-L-methionine + tRNAPhe = 5'-methylthioadenosine + tRNA 3-(3-amino-3-carboxypropyl) uridine
-
show the reaction diagram
ATP + rRNA = diphosphate + ?
-
show the reaction diagram
tRNACys + 2 CTP + ATP = tRNACys with 3'-CCA end + 3 diphosphate
-
show the reaction diagram
rRNA + H2O = ?
-
show the reaction diagram
tRNATyr + H2O = ?
-
show the reaction diagram
ribosomal RNA + H2O = smaller precursor rRNA
-
show the reaction diagram
rRNA + H2O = ?
-
show the reaction diagram
ATP + L-leucine + tRNAIle = AMP + diphosphate + L-leucyl-tRNALeu
-
show the reaction diagram
ATP + L-glutamate + tRNAAsp = AMP + diphosphate + L-glutamyl-tRNAAsp
-
show the reaction diagram
ATP + L-glutamate + tRNAGlu = AMP + diphosphate + L-glutamyl-tRNAGlu
-

Product in Enzyme-catalyzed Reactions (156 results)

EC NUMBER
LITERATURE
REACTION DIAGRAM
REACTION
ENZYME 3D STRUCTURE
show the reaction diagram
L-alanyl-tRNAAla + phosphatidylglycerol = tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
-
show the reaction diagram
glycyl-tRNAGly + N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine = tRNAGly + N-acetylmuramoyl-L-alanyl-D-isoglutaminyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine
-
-
show the reaction diagram
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + glycyl-tRNAGly = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-penta-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + tRNAGly
-
-
show the reaction diagram
guanine + tRNAguanine = tRNAguanine + guanine
-
-
show the reaction diagram
tRNAqueuine + guanine = tRNAguanine + queuine
-
-
show the reaction diagram
pre-tRNAArg + H2O = tRNAArg + 3'-leader of tRNA
-
-
show the reaction diagram
AMP + diphosphate + L-tyrosyl-tRNATyr = ATP + L-tyrosine + tRNATyr
-
-
show the reaction diagram
AMP + diphosphate + L-methionyl-tRNAMet = ATP + L-methionine + tRNAMet
-
-
show the reaction diagram
AMP + diphosphate + L-seryl-tRNASer = ATP + L-serine + tRNASer
-
-
show the reaction diagram
AMP + diphosphate + L-cysteinyl-tRNACys = ATP + L-cysteine + tRNACys
-
-
show the reaction diagram
AMP + diphosphate + L-threonyl-tRNAThr = ATP + L-threonine + tRNAThr
-

Activator in Enzyme-catalyzed Reactions (4 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE

Inhibitor in Enzyme-catalyzed Reactions (6 results)

COMMENTARY
EC NUMBER
LITERATURE
ENZYME 3D STRUCTURE
inhibits binding of 16S rRNA to RUMT
-
tRNALys from Escherichia coli
-
weak inhibitor of T4 mRNA-degradation
-
specific competitive inhibition
-
-
-

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (383 results)

COMMENTARY
EC NUMBER
LITERATURE
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
-
29.6
-
37°C, presence of 50 mM KCl, 1 mM MgCl2
62
-
pH 7.4, 28°C, Artemia salina ribosomes, A subunit treated with trypsin, urea and dithiothreitol
10.7
-
pH 7.4, 28°C, Artemia salina ribosomes, A subunit treated with urea and dithiothreitol
11.6
-
pH 7.4, 28°C, Artemia salina ribosomes, holotoxin treated with trypsin, urea and dithiothreitol
12.8
-
pH 7.4, 28°C, Artemia salina ribosomes, untreated A subunit
0.0933
-
pH 7.4, 28°C, Artemia salina ribosomes, untreated holotoxin
0.01
-
pH 7.6, 37°C, Escherichia coli naked 23 S rRNA
0.0005
-
pH 7.6, 37°C, rat liver 80S ribosome
29.6
-
pH 7.6, 37°C, rat liver naked 28 S rRNA
0.000333
-
ricin A chain, 37°C, presence of 50 mM KCl, 1 mM MgCl2
29.6
-
substrate from yeast, in absence of Mg2+
0.00833
-
substrate from yeast, intronless, limiting
0.00833
-
substrate from yeast, limiting
0.00667
-
55°C, pH 8.0
0.29
-
pH 7.2, 37°C, tRNATyr aminoacylation activity, recombinant lysine acetylated enzyme mutant 144AcK
1.17
-
pH 7.2, 37°C, tRNATyr aminoacylation activity, recombinant lysine acetylated enzyme mutant 355AcK
1.09
-
pH 7.2, 37°C, tRNATyr aminoacylation activity, recombinant lysine acetylated enzyme mutant 85AcK
0.15
-
pH 7.2, 37°C, tRNATyr aminoacylation activity, recombinant wild-type enzyme
1.32
-
wild type enzyme, at 55°C, in 100 mM HEPES-NaOH (pH 8.0), 10 mM MgCl2, 10 mM KCl
0.29
-
aminoacylation reaction, C-terminally truncated mutant
3.6
-
aminoacylation reaction, mutant D666A
3.1
-
aminoacylation reaction, mutant M665
4.4
-
aminoacylation reaction, wild-type
2.8
-
ATP-diphosphate exchange reaction, C-terminally truncated mutant
47
-
ATP-diphosphate exchange reaction, mutant D666A
23
-
ATP-diphosphate exchange reaction, mutant M665
2
8
ATP-diphosphate exchange reaction, wild-type
42
-
C-terminal extension, pH 7.5, 25°C
0.09
-
catalytic domain, pH 7.5, 25°C
2.4
-
in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, truncated mutant
0.36
-
in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, wild-type enzyme
0.29
-
mutant A247E, aminoacylation reaction
0.057
-
mutant D229E, second step of reaction, tRNA aminoacylation
0.023
-
mutant G223C, aminoacylation reaction
0.037
-
mutant G54A/A64P, aminoacylation reaction
0.001
-
mutant G54S, aminoacylation reaction
0.009
-
mutant G54S, second step of reaction, tRNA aminoacylation
0.01
-
mutant G54S/L203S, second step of reaction, tRNA aminoacylation
0.011
-
mutant I57N, aminoacylation reaction
0.023
-
mutant I57N, second step of reaction, tRNA aminoacylation
0.017
-
mutant I57N/A247E, aminoacylation reaction
0.008
-
mutant I57N/G54S, aminoacylation reaction
0.002
-
mutant I57N/H101N, second step of reaction, tRNA aminoacylation
0.013
-
mutant I57N/I238F, aminoacylation reaction
0.066
-
mutant I57N/V242F, aminoacylation reaction
0.124
-
mutant K860A, pH 7.5, 25°C
0.85
-
mutant K863A, pH 7.5, 25°C
0.22
-
mutant K866A, pH 7.5, 25°C
0.23
-
mutant K880A, pH 7.5, 25°C
mutant L213W, aminoacylation reaction
0.54
-
mutant R857A, pH 7.5, 25°C
0.47
-
mutant V108M, aminoacylation reaction
0.74
-
mutant V216A, second step of reaction, tRNA aminoacylation
0.055
-
mutant V216F/L203S, second step of reaction, tRNA aminoacylation
0.006
-
pH 7.5, 25°C, mutant D369A
0.037
-
pH 7.5, 25°C, mutant D369K/K295D
0.05
-
pH 7.5, 25°C, mutant D369N
0.11
-
pH 7.5, 25°C, mutant K295V
0.3
-
pH 7.5, 25°C, mutant KA295V
0.042
-
pH 7.5, 25°C, wild-type enzyme
1
-
pH 8.2, 37°C, human mitochondrial wild-type tRNAMet, recombinant enzyme
0.021
-
pH 8.2, 37°C, initiator tRNA from Escherichia coli, recombinant enzyme
11
-
pH 8.2, 37°C, mitochondrial tRNAMet from Bos taurus, recombinant enzyme
0.019
-
recombinant C-terminally truncated enzyme, 37°C
6.85
-
recombinant cytoplasmic isozyme as GST-fusion protein, pH 7.8, 25°C
1
-
recombinant cytoplasmic isozyme, pH 7.8, 25°C
1.3
-
recombinant mitochondrial isozyme as GST-fusion protein, pH 7.8, 25°C
0.2
-
substrate from Escherichia coli, 37°C
wild-type enzyme
3.3
-
wild-type enzyme in the multi-enzyme complex, pH 7.5, 25°C
0.46
-
wild-type enzyme, pH 7.5, 25°C
0.15
-
wild-type, aminoacylation reaction
0.49
-
wild-type, second step of reaction, tRNA aminoacylation
0.08
-
30°C, aminoacylation
0.057
-
37°C, aminoacylation
0.125
-
aminoacylation
1.37
-
aminoacylation, mutant enzymes
0.057
0.65
aminoacylation, wild-type enzyme
at pH 7.6, temperature not specified in the publication
13.7
-
H270G mutant, aminoacylation
0.18
-
in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication
1.1
-
mitochondrial isozyme, pH 7.5, 30°C
mutant E273A
0.55
-
mutant E273A/D277A/K280A
1.4
-
mutant E338A
0.059
-
mutant enzyme DELTAG254-N261, at pH 7.5 and 37°C
0.2
-
mutant enzyme DELTAG75-N97, at pH 7.5 and 37°C
0.025
-
mutant enzyme DELTAG75-N97/DELTAG254-N261, at pH 7.5 and 37°C
0.005
-
mutant G340V/G341A
0.000000011
-
mutant K164A
0.0087
-
mutant N435A
0.435
-
mutant N435A/S437A
0.361
-
mutant N435A/S437A/W396A
0.175
-
mutant R147A
0.15
-
mutant R267A
0.032
-
mutant R347A
0.00026
-
mutant S437A
0.396
-
mutant ScSerRSDELTAC13 (deletion of 13 C-terminal residues), using yeast tRNA
0.54
-
mutant SerRS11, pH 7.2, 30°C
0.4
-
mutant SerRS12, pH 7.2, 30°C
0.46
-
mutant W396A
0.239
-
mutant ZmcSerRSDELTAC12 (deletion of 12 C-terminal residues), using maize tRNA
1.28
-
mutant ZmcSerRSDELTAC18 (deletion of 18 C-terminal residues), using maize tRNA
1.43
-
pH 7.5, 50°C, recombinant MtSerRS
0.0073
-
pH 7.5, 50°C, recombinant MtSerRS in presence of recombinant MtArgRS
0.1
-
pH 7.6, 37°C
0.062
-
Ser, , aminoacylation, truncated enzyme
0.41
-
wild type enzyme, at pH 7.5 and 37°C
0.43
-
wild-type
wild-type enzyme, pH 7.2, 30°C
0.28
-
wild-type, using maize tRNA
1.48
-
wild-type, using yeast tRNA
0.58
-
Escherichia coli native tRNAAsp
0.133
-
Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500
0.22
-
human mitochondrial tRNAAsp
0.1
-
native enzyme, at pH 7.5 and 25°C
0.004
-
pH 6.0, 60°C, mutant enzyme K85P
pH 6.0, 60°C, mutant enzyme W26HK85P
pH 6.0, 60°C, mutant enzyme W26H
pH 6.0, 60°C, mutant enzyme W26H/K85P
pH 6.0, 60°C, wild-type enzyme
0.061
-
pH 7.2, 70°C
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme A471T
8
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme E118K
79
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme E93K
0.05
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), mutant enzyme L30F
12
-
pH 7.5, 37°C, aminoacylation of tRNAAsp(QUC), wild-type enzyme
12
-
pH 7.5, 37°C, recombinant mutant C152F
0.0016
-
pH 7.5, 37°C, recombinant mutant Q184K
0.00016
-
pH 7.5, 37°C, recombinant wild-type enzyme
0.027
-
pH 7.8, 28°C
0.55
-
Plasmodium falciparum aspartyl-tRNA synthetase, mutated RNA binding motif
0.01
-
Plasmodium falciparum wild type aspartyl-tRNA synthetase, amino acids 49-626
0.2
-
recombinant enzyme, at pH 7.5 and 25°C
recombinant mutant enzyme, pH 7.2, 37°C
recombinant wild-type enzyme, pH 7.2, 37°C
wild-type enzyme, acylation activity, pH 7.5, 37°C
9.3
-
aminoacylation
wild type isoform GlyRS1 without insertion peptide, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C
0.19
-
wild type isoform GlyRS1, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C
0.33
-
wild type isoform GlyRS2, in 50 mM HEPES (pH 7.5), 50 mM KCl, 15 mM MgCl2, 5 mM dithiothreitol, at 25°C
0.73
-
50°C, pH 7.5
1.8
-
pH 7.0, 37°C
0.58
-
pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, aminoacylation
0.0091
-
pH 7.0, 37°C, recombinant wild-type enzyme, aminoacylation
0.239
-
pH 7.0, 55°C, C-terminal deletion mutant
0.4
-
pH 7.0, 55°C, wild-type
1.6
-
presence of bovine serum albumine and leucyl-tRNA synthetase, 50°C, pH 7.5
6.9
-
presence of bovine serum albumine, 50°C, pH 7.5
2.9
-
presence of leucyl-tRNA synthetase, 50°C, pH 7.5
5.1
-
single turnover of the transfer step
10.8
-
single turnover overall aminoacylation
9.8
-
steady-state
1.8
-
30°C, pH 7.0, mutant enzyme V27E
0.68
-
30°C, pH 7.0, wild-type enzyme
2.9
-
37°C, wild-type enzyme
2.46
-
aminoacylation reaction, recombinant C28S/C209S mutant enzyme
0.005
-
aminoacylation reaction, recombinant His-tagged Co2+-wild-type enzyme
0.5
-
aminoacylation reaction, recombinant His-tagged Zn2+-wild-type enzyme
2.5
-
aminoacylation reaction, recombinant W205Y mutant enzyme
0.007
-
aminoacylation reaction, recombinant Zn2+-wild-type enzyme
0.9
-
mutant enzyme E354A, mutant tRNACys(G15-C48)
0.036
-
mutant enzyme E354A, wild-type tRNACys(G15-G48)
0.1
-
mutant enzyme E354Q, mutant tRNACys(G15-C48)
0.074
-
mutant enzyme E354Q, wild-type tRNACys(G15-G48)
0.14
-
mutant enzyme N351A, mutant tRNACys(G15-C48)
0.39
-
mutant enzyme N351A, wild-type tRNACys(G15-G48)
0.59
-
mutant enzyme N351D, mutant tRNACys(G15-C48)
0.03
-
mutant enzyme N351D, wild-type tRNACys(G15-G48)
0.094
-
pH 7.0, 37°C
pH 7.5, 40°C, recombinant mutant D239A/D240A
0.06
-
pH 7.5, 40°C, recombinant mutant D417A/E420A
0.014
-
pH 7.5, 40°C, recombinant mutant D435A/D436A
0.03
-
pH 7.5, 40°C, recombinant wild-type enzyme
0.11
-
recombinant His-tagged deletion mutant, amino acid activation step
0.2
-
recombinant His-tagged wild-type full-length enzyme, amino acid activation step
2.3
-
steady-state
2.9
-
wild-type enzyme, mutant tRNACys(G15-C48)
0.45
-
wild-type enzyme, wild-type tRNACys(G15-G48)
2.46
-
in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C
in presence of the GluRS-Arc1p complex
0.016
-
in the presence of diphosphatase, in 100 mM HEPES-KOH, pH 7.2, 30 mM KOH, 12 mM MgCl2, 2 mM dithiothreitol, at 37°C
pH 7.3, 37°C
0.408
-
pH 7.5, 37°C
0.8
-
pH and temperature not specified in the publication
1.5
-
wild type enzyme, pH and temperature not specified in the publication
0.04
-
wild-type
0.0058
-
wild-type tRNA substrate, pH 7.2, 37°C
3
-
-
3.3
-
pH and temperature not specified in the publication
1.4
-
recombinant mutant G45V, pH 7.2, 37°C
0.0148
-
recombinant mutant H175A, pH 7.2, 37°C
0.0033
-
recombinant mutant Y57H, pH 7.2, 37°C
0.002
-
recombinant wild-type enzyme, pH 7.2, 37°C
0.203
-
25°C, pH 7.5, mammalian tRNAArg, aminoacylation
0.00016
-
25°C, pH 7.5, native tRNAArg from beef liver, mutant enzyme DELTA1-73
2.6
-
25°C, pH 7.5, native tRNAArg from beef liver, wild type enzyme
1.8
-
25°C, pH 7.5, tRNAArg from Saccharomyces cerevisiae, aminoacylation
0.0045
-
37°C, pH 7.5, arginylation, full-length enzyme
0.32
-
37°C, pH 7.5, arginylation, mutant enzyme DELTA1-72
0.81
-
argS1 mutant, pH 7.5, 37°C
2.8
-
mutant enzyme DELTANhcArgRS, in 50 mM Tris-HCl (pH 7.5)
0.8
-
pH 7.5, 37°C
17
-
pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, absence of hemin, Escherichia coli tRNAArg
16.5
-
pH 7.5, 37°C, recombinant, truncated DELTANhcArgRS, presence of hemin, Escherichia coli tRNAArg
2.5
-
pH 7.5, 50°C, recombinant MtArgRS
0.026
-
pH 7.5, 50°C, recombinant MtArgRS in presence of recombinant MtSerRS
0.034
-
transfer RNA isoacceptor for arginine UCU
wild type enzyme, in 50 mM Tris-HCl (pH 7.5)
0.36
-
22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme E153D, aminoacylation
0.03
-
22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme E153K, aminoacylation
0.02
-
22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149D/E153R, aminoacylation
0.016
-
22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149E, aminoacylation
0.67
-
22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149G, aminoacylation
0.78
-
22°C, pH 7.5, tRNATrp from Bacillus subtilis, mutant enzyme K149R, 0.32 aminoacylation
0.32
-
22°C, pH 7.5, tRNATrp from Bacillus subtilis, wild-type enzyme, aminoacylation
0.32
-
pH 7.5, 22°C, deletion 108-122 mutant
pH 7.5, 22°C, deletion 234-238 mutant
pH 7.5, 22°C, wild-type enzyme
0.9
-
pH 7.5, 30°C, mutant V85A
0.48
-
pH 7.5, 30°C, mutant V85A/V90A
0.047
-
pH 7.5, 30°C, mutant V85E
0.02
-
pH 7.5, 30°C, mutant V85K
0.0009
-
pH 7.5, 30°C, mutant V85L
pH 7.5, 30°C, mutant V85S
0.028
-
pH 7.5, 30°C, mutant V90A
1.39
-
pH 7.5, 30°C, mutant V90S
0.063
-
pH 7.5, 30°C, recombinant enzyme
1
-
pH 7.5, 30°C, recombinant wild-type enzyme
1.33
-
pH 7.5, 30°C, wild-type enzyme
1.37
-
pH 7.5, 60°C
recombinant mutant
13.9
-
recombinant truncated mutant
recombinant wild-type
aminoacylation reaction, recombinant mitochondrial isozyme, pH 7.5, 37°C
0.11
-
at pH 7.5 and 37°C
mutant enzyme D289Y, at pH 8.5 and 37°C
0.08
-
mutant enzyme P49A, at pH 8.5 and 37°C
0.14
-
mutant enzyme R383C, at pH 8.5 and 37°C
0.07
-
mutant enzyme R387Q, at pH 8.5 and 37°C
0.14
-
mutant enzyme T210M, at pH 8.5 and 37°C
0.22
-
pH 7.2, 37°C, full-length PheRSA294G, substrate from Escherichia coli
0.82
-
pH 7.2, 37°C, full-length PheRSA294G, substrate from Saccharomyces cerevisiae
0.077
-
pH 7.2, 37°C, truncated mutant PheRSDELTAB2A294G, substrate from Escherichia coli
0.78
-
pH 7.2, 37°C, truncated mutant PheRSDELTAB2A294G, substrate from Saccharomyces cerevisiae
0.11
-
wild type enzyme, at pH 8.5 and 37°C
0.19
-
mutant E83A, pH 7.5, 37°C
0.0004
-
mutant E83Q, pH 7.5, 37°C
0.01
-
mutant enzyme S365N, at pH 7.5 and 25°C
0.3
-
mutant enzyme V155G, at pH 7.5 and 25°C
3.05
-
mutant enzyme Y330C, at pH 7.5 and 25°C
0.56
-
mutant Q127A, pH 7.5, 37°C
0.048
-
mutant R159H, pH 7.5, 37°C
0.0006
-
pH 7.2, 37°C, wild-type substrate
0.417
-
pH 8.0, 37°C, aminoacylation
wild type enzyme, at pH 7.5 and 25°C
5.4
-
wild-type Caulobacter crescentus tRNAHis, pH 7.5, 30°C
4
-
wild-type, pH 7.5, 37°C
2.02
-
at pH 7.2 and 37°C
0.043
-
in 50 mM HEPES-NaOH, pH 7.2, 10 mM MgCl2, at 37°C
0.5
-
37°C, pH 7.0, mutant enzyme H28Q
37°C, pH 7.0, mutant enzyme P77K
37°C, pH 7.0, mutant enzyme P77K/H28Q
37°C, pH 7.0, wild-type enzym
0.51
-
37°C, pH 7.0, wild-type enzyme
0.15
-
AspRS1
7.2
-
AspRS2
at pH 7.2 and 37°C
at pH 8.0 and 37°C
pH 7.5, recombinant mutant L81N enzyme
pH 7.5, recombinant mutant L86M enzyme
pH 7.5, recombinant wild-type enzyme
homologous tRNAGln
0.04
-
homologous tRNAGlu
0.05
-
recombinant hybrid GlnRS S1/L1/L2, pH not specified in the publication, temperature not specified in the publication
tRNAGln from E. coli
0.07
-
pH 7.2, 37°C, wild-type tRNAPyl
0.19
-
50°C, pH 7.6, tRNACys containing the (pyrrole-2-carbaldehyde)UA anticodon, mutant enzyme D418N D420N T423V
0.0471
-
50°C, pH 7.6, tRNACys containing the C(pyrrole-2-carbaldehyde)A anticodon, mutant enzyme D418N D420N T423V
0.048
-
50°C, pH 7.6, tRNACys with the GCA anticodon, wild-type enzyme
0.115
-
60°C, pH 6.0, steady-state kinetics
0.07
-
at pH 7.5 and 60°C
0.07
-
pH 7.5, 37°C, recombinant enzyme
0.12
-
pH 7.5, 37°C, recombinant enzyme, m1G37 tRNACys
0.24
-
pH 7.5, 37°C, recombinant enzyme, native tRNACys
1
-
pH 7.6, 50°C, mutant D418N/D420N/T423V
0.008
-
pH 7.6, 50°C, wild-type enzyme
0.115
-
-
0.037
0.23
pH 7.2, 37°C, ThrRS, aminoacylation with Thr
0.05
-
pH 7.2, 37°C, tRNAThr1(G-1), MST1
0.00157
-
pH 7.2, 37°C, tRNAThr1, MST1
0.0467
-
pH 7.2, 37°C, tRNAThr2, MST1
0.0383
-
tRNAThr of E. coli
tRNAThr of Thermus thermophilus
0.52
0.7
pH 7.5, 30°C, deacylation, recombinant mutant D334A
pH 7.5, 30°C, deacylation, recombinant wild-type enzyme
wild-type enzyme
3.1
-
18S synthetase complex
2.7
-
free enzyme
5.5
-
human wild-type tRNALys, 20°C, wild-type enzyme
1.7
-
human wild-type tRNALys, 30°C, N-terminally truncated mutant enzyme
0.38
-
human wild-type tRNALys, 30°C, wild-type enzyme
2
-
wild-type tRNALys
0.34
-
-
2
-
AlaRS, aminoacylation
1.5
-
mutant AlaRS(K73A), aminoacylation
1.4
-
mutant AlaRS(K73E), aminoacylation
0.24
-
mutant AlaRS(K73Q), ATP-diphosphate exchange
1.7
-
mutant substrate from Escherichia coli, pH 7.5, 37°C
0.19
-
native dual specific tRNAPhe substrate from Saccharomyces cerevisiae, pH 7.5, 37°C
0.25
-
pH 7.6, 37°C, 0.00025 mg recombinant His-tagged enzyme
0.2
-
pH 7.6, 50°C
2.2
-
wild-type substrate from Escherichia coli, pH 7.5, 37°C
0.44
-
aminoacylation reaction with L-valine, K277A mutant enzyme, pH 7.5, 25°C
1.5
-
aminoacylation reaction with L-valine, wild-type enzyme, pH 7.5, 25°C
1.7
-
ATP-diphosphate exchange reaction, K277A mutant enzyme, pH 7.5, 25°C
50
-
ATP-diphosphate exchange reaction, wild-type enzyme, pH 7.5, 25°C
51
-
C-terminally truncated mutant enzyme, pH 7.7, 65°C
0.36
-
R818A/R843A mutant enzyme, pH 7.7, 65°C
4.3
-
wild-type enzyme, pH 7.7, 65°C
6.7
-
pH 7.2, 37°C
0.04
-

KM Value (491 results)

COMMENTARY
EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
LITERATURE
mutant enzyme S90C
0.0097
-
wild-type enzyme
0.00032
-
37°C, presence of 50 mM KCl, 1 mM MgCl2
0.000055
-
pH 7.4, 28°C, Artemia salina ribosomes, A subunit treated with trypsin, urea and dithiothreitol
0.00137
-
pH 7.4, 28°C, Artemia salina ribosomes, A subunit treated with urea and dithiothreitol
0.00096
-
pH 7.4, 28°C, Artemia salina ribosomes, holotoxin treated with trypsin, urea and dithiothreitol
0.00098
-
pH 7.4, 28°C, Artemia salina ribosomes, untreated A subunit
0.00091
-
pH 7.4, 28°C, Artemia salina ribosomes, untreated holotoxin
0.00084
-
pH 7.6, 35°C, 80S ribosome, cinnamomin
0.0011
-
pH 7.6, 35°C, naked RNA, cinnamomin
0.0015
-
pH 7.6, 37°C, Escherichia coli naked 23 S rRNA
0.0033
-
pH 7.6, 37°C, rat liver 80S ribosome
0.0026
-
pH 7.6, 37°C, rat liver naked 28 S rRNA
0.0058
-
ricin A chain, 37°C, presence of 50 mM KCl, 1 mM MgCl2
0.0026
-
Pus1, intronless yeast tRNAIle
0.00074
-
Pus1, yeast tRNAVal
Pus1, yeast tRNAVal, in the presence of 5 mM EDTA and in the absence of Mg2+
0.0015
-
30°C, purified recombinant His-tagged enzyme
0.0009
-
55°C, pH 8.0
0.00022
-
acylation
0.00052
-
aminoacylation reaction, pH 8.0, wild-type enzyme
0.0025
-
chloroplasts
0.000022
-
cytoplasm
0.0000898
-
in absence of KCl, pH 7.4, 30°C
0.000022
-
in presence of 100 mM KCl, pH 7.4, 30°C
0.000093
-
in presence of 150 mM KCl, pH 7.4, 30°C
0.00024
-
in presence of 50 mM KCl, pH 7.4, 30°C
0.000037
-
pH 7.2, 37°C, tRNATyr aminoacylation activity, recombinant lysine acetylated enzyme mutant 144AcK
0.00042
-
pH 7.2, 37°C, tRNATyr aminoacylation activity, recombinant lysine acetylated enzyme mutant 355AcK
0.00048
-
pH 7.2, 37°C, tRNATyr aminoacylation activity, recombinant lysine acetylated enzyme mutant 85AcK
0.00045
-
pH 7.2, 37°C, tRNATyr aminoacylation activity, recombinant wild-type enzyme
0.00037
-
pH 7.6, 37°C, human mitochondrial tRNATyr
0.0048
-
pH 7.6, 37°C, native Escherichia coli tRNATyr
0.00052
-
wild type enzyme, at 55°C, in 100 mM HEPES-NaOH (pH 8.0), 10 mM MgCl2, 10 mM KCl
0.00022
-
aminoacylation reaction, C-terminally truncated mutant
0.0058
-
aminoacylation reaction, mutant D666A
0.0064
-
aminoacylation reaction, mutant M665
0.0087
-
aminoacylation reaction, wild-type
0.0044
-
ATP-diphosphate exchange reaction, C-terminally truncated mutant
0.022
-
ATP-diphosphate exchange reaction, mutant D666A
0.029
-
ATP-diphosphate exchange reaction, mutant M665
0.034
-
ATP-diphosphate exchange reaction, wild-type
0.02
-
C-terminal extension, pH 7.5, 25°C
0.0022
-
catalytic domain, pH 7.5, 25°C
0.032
-
in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, truncated mutant
0.0065
-
in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, wild-type enzyme
0.00066
-
mutant A247E, aminoacylation reaction
0.0013
-
mutant D229E, second step of reaction, tRNA aminoacylation
0.00065
-
mutant G223C, aminoacylation reaction
0.0025
-
mutant G54A/A64P, aminoacylation reaction
0.00057
-
mutant G54S, aminoacylation reaction
0.00027
-
mutant G54S, second step of reaction, tRNA aminoacylation
0.00102
-
mutant G54S/L203S, second step of reaction, tRNA aminoacylation
0.00064
-
mutant I57N, aminoacylation reaction
0.00093
-
mutant I57N, second step of reaction, tRNA aminoacylation
0.0026
-
mutant I57N/A247E, aminoacylation reaction
0.00045
-
mutant I57N/G54S, aminoacylation reaction
0.00041
-
mutant I57N/H101N, second step of reaction, tRNA aminoacylation
0.00121
-
mutant I57N/I238F, aminoacylation reaction
0.00089
-
mutant I57N/V242F, aminoacylation reaction
0.01
-
mutant K860A, pH 7.5, 25°C
0.0172
-
mutant K863A, pH 7.5, 25°C
0.0033
-
mutant K880A, pH 7.5, 25°C
0.0163
-
mutant L213W, aminoacylation reaction
0.0017
-
mutant R857A, pH 7.5, 25°C
0.0057
-
mutant V108M, aminoacylation reaction
0.0032
-
mutant V216A, second step of reaction, tRNA aminoacylation
0.0008
-
mutant V216F/L203S, second step of reaction, tRNA aminoacylation
0.00096
-
pH 7.5, 25°C, mutant D369A
0.018
-
pH 7.5, 25°C, mutant D369K/K295D
0.006
-
pH 7.5, 25°C, mutant D369N
0.026
-
pH 7.5, 25°C, mutant K295V
0.0177
-
pH 7.5, 25°C, mutant KA295V
0.0102
-
pH 7.5, 25°C, wild-type enzyme
0.0039
-
pH 8.2, 37°C, human mitochondrial wild-type tRNAMet, recombinant enzyme
0.00016
-
pH 8.2, 37°C, initiator tRNA from Escherichia coli, recombinant enzyme
0.0021
-
pH 8.2, 37°C, mitochondrial tRNAMet from Bos taurus, recombinant enzyme
0.00015
-
recombinant C-terminally truncated enzyme, 37°C
1
-
recombinant cytoplasmic isozyme as GST-fusion protein, pH 7.8, 25°C
0.015
-
recombinant cytoplasmic isozyme, pH 7.8, 25°C
0.009
-
recombinant mitochondrial isozyme as GST-fusion protein, pH 7.8, 25°C
0.0004
-
substrate from Escherichia coli, 37°C
0.0032
-
wild-type enzyme
0.0009
-
wild-type enzyme in the multi-enzyme complex and mutant K866A, pH 7.5, 25°C
0.0039
-
wild-type enzyme, pH 7.5, 25°C
0.0035
-
wild-type, aminoacylation reaction
0.001
-
wild-type, second step of reaction, tRNA aminoacylation
0.00055
-
-
0.0005
-
30°C, aminoacylation
0.012
-
37°C, aminoacylation
0.0086
-
aminoacylation
0.24
-
aminoacylation, truncated enzyme
0.00005
-
aminoacylation, wild-type enzyme
at pH 7.6, temperature not specified in the publication
0.0073
-
H270G mutant, aminoacylation
0.41
-
in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication
0.0008628
-
mitochondrial isozyme, pH 7.5, 30°C
mutant E273A
0.0042
-
mutant E273A/D277A/K280A
0.0047
-
mutant E338A
0.0021
-
mutant enzyme DELTAG254-N261, at pH 7.5 and 37°C
0.00051
-
mutant enzyme DELTAG75-N97, at pH 7.5 and 37°C
0.00033
-
mutant enzyme DELTAG75-N97/DELTAG254-N261, at pH 7.5 and 37°C
0.00013
-
mutant G340V/G341A
0.0067
-
mutant K164A
0.0024
-
mutant N435A
0.283
-
mutant N435A/S437A
0.509
-
mutant N435A/S437A/W396A
1.191
-
mutant R147A
0.0146
-
mutant R267A
0.0034
-
mutant R347A
0.0063
-
mutant S437A
0.412
-
mutant ScSerRSDELTAC13 (deletion of 13 C-terminal residues), using yeast tRNA
0.00065
-
mutant W396A
0.76
-
mutant ZmcSerRSDELTAC12 (deletion of 12 C-terminal residues), using maize tRNA
0.00065
-
mutant ZmcSerRSDELTAC18 (deletion of 18 C-terminal residues), using maize tRNA
0.00066
-
pH 7.5, 50°C, recombinant MtSerRS
0.00561
-
pH 7.5, 50°C, recombinant MtSerRS in presence of recombinant MtArgRS
0.002
-
pH 7.6, 37°C
0.00054
-
substrate from Bombyx mori
0.00025
-
substrate from E. coli
0.02
-
substrate from rabbit liver
0.00026
-
substrate from yeast
0.00024
-
wild type enzyme, at pH 7.5 and 37°C
0.00077
-
wild-type
wild-type enzyme, mutant SerRS11, and mutant SerRS12, pH 7.2, 30°C
0.00004
-
wild-type, using maize tRNA
0.00058
-
wild-type, using yeast tRNA
0.00071
-
aspartyl-tRNA synthetase as a component of the multisynthetase complex or recombinant native enzyme
0.0005
-
Escherichia coli native tRNAAsp
0.00015
-
Homo sapiens wild type aspartyl-tRNA synthetase, amino acids 1-500
0.00016
-
human mitochondrial tRNAAsp
0.00013
-
native enzyme
0.000013
-
native enzyme, at pH 7.5 and 25°C
0.0011
-