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Ligand 12-ketochenodeoxycholic acid Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Basic Ligand Information Molecular Structure
C2 4 H3 8 O5
12-ketochenodeoxycholic acid
MIHNUBCEFJLAGN-DMMBONCOSA-N
(3alpha,5beta,7alpha)-3,7-dihydroxy-12-oxocholan-24-oic acid, 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate, 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid, 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate, 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate, 12-oxochenodeoxycholic acid
Roles as Enzyme Ligand
In Vivo Substrate in Enzyme-catalyzed Reactions (1 result)Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
12-ketochenodeoxycholic acid + NADH = cholic acid + NAD+
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In Vivo Product in Enzyme-catalyzed Reactions (2 results)Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cholate + NAD+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
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cholate + NADP+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
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Substrate in Enzyme-catalyzed Reactions (4 results)
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12-ketochenodeoxycholic acid + NAD+ = 7,12-diketochenodeoxycholic acid + NADH
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12-ketochenodeoxycholic acid + NADH = cholic acid + NAD+
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12-oxochenodeoxycholic acid + NADP+ = (3alpha,5beta,7alpha,12alpha)-3,7,12-trihydroxycholan-24-oic acid + NADPH + H+
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3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate + NADPH = 3alpha,7alpha,12beta-trihydroxy-5beta-cholanoate + NADP+
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Product in Enzyme-catalyzed Reactions (7 results)
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(3alpha,5beta)-3-hydroxy-7,12-dioxocholan-24-oic acid + NADH = (3alpha,5beta,7alpha)-3,7-dihydroxy-12-oxocholan-24-oic acid + NAD+
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7,12-diketolithocholic acid + NADH = 12-ketochenodeoxycholic acid + NAD+
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cholic acid + NADP+ = 12-ketochenodeoxycholic acid + NADPH
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cholic acid + NAD(P)+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oic acid + NAD(P)H
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cholate + NAD+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
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cholate + NADP+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADH + H+
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cholate + NADP+ = 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+
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Inhibitor in Enzyme-catalyzed Reactions (1 result)
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inhibition of the ADH from Lactobacillus brevis by bile acids, acetone prevents partially
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Enzyme Kinetic Parameters
KM Value (2 results)
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References & Links Literature References (6)
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Purification and properties of three kinds of alpha-hydroxysteroid dehydrogenase from Brevibacterium fuscum DC33
1988
Kinoshita, S.; Kadota, K.; Inoue, T.; Sawada, H.; Taguchi, H.
J. Ferment. Technol.
66
145-152
The specificity of a 7alpha-hydroxy steroid dehydrogenase from Escherichia coli
1976
Haslewood, E.S.; Haslewood, G.A.D.
Biochem. J.
157
207-210
Characterization of NADP-dependent 12beta-hydroxysteroid dehydrogenase from Clostridium paraputrificum
1988
Edenharder, R.; Pfutzner, A.
Biochim. Biophys. Acta
962
362-370
12 alpha-hydroxysteroid dehydrogenase from Clostridium group P, strain C 48-50. Production, purification and characterization
1991
Braun, M.; Lunsdorf, H.; Buckmann, A.F.
Eur. J. Biochem.
196
439-450
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Bile acids induction of 12 alpha-hydroxysteroid dehydrogenase in Brevibacterium fuscum
1993
Panichajakul, S.; Tawat, K.; Yoshida, T.; Taguchi, H.
Microb. Util. Renewable Resour.
8
112-118
Exploitation of the alcohol dehydrogenase-acetone NADP-regeneration system for the enzymatic preparative-scale production of 12-ketochenodeoxycholic acid
2006
Fossati, E.; Polentini, F.; Carrea, G.; Riva, S.
Biotechnol. Bioeng.
93
1216-1220
Links to other databases for 12-ketochenodeoxycholic acid
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