print visible entries

print all entries

Ligand ent-isokaurene

Basic Ligand Information

Molecular Structure

Show all BRENDA pathways known for ent-isokaurene

close all tables

open all tables

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (2 results)

top print hide

Show Natural Substrate (2 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.14.76

ent-isokaurene + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] = ent-isokaurene-2beta,3beta-diol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O

746144

-

1.14.14.76

ent-isokaurene + O2 + [reduced NADPH-hemoprotein reductase] = ent-isokauren-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O

744147

-

1.14.14.76

2 entries

In Vivo Product in Enzyme-catalyzed Reactions (3 results)

top print hide

Show Natural Product (3 entries)

EC NUMBER

PROVEN IN VIVO REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.2.3.103

ent-copalyl diphosphate = ent-isokaur-15-ene + diphosphate

-

-

4.2.3.103

ent-copalyl diphosphate = ent-isokaurene + diphosphate

-

-

4.2.3.103

2 entries

4.2.3.19

ent-copalyl diphosphate = ent-kaur-15-ene + diphosphate

-

-

Substrate in Enzyme-catalyzed Reactions (5 results)

top print hide

Show Substrate (5 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

1.14.14.123

ent-isokaurene + [reduced NADPH-hemoprotein reductase] + O2 = ?

717875

-

1.14.14.76

ent-isokaurene + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] = ent-isokaurene-2beta,3beta-diol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O

746144

-

1.14.14.76

ent-isokaurene + O2 + [reduced NADPH-hemoprotein reductase] = ent-isokauren-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O

744147

-

1.14.14.76

ent-isokaurene + [reduced NADPH-hemoprotein reductase] + O2 = ent-2alpha-hydroxyisokaurene + [oxidized NADPH-hemoprotein reductase] + H2O

717607

-

1.14.14.76

3 entries

1.14.14.86

ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O

746311

-

Product in Enzyme-catalyzed Reactions (4 results)

top print hide

Show Product (4 entries)

EC NUMBER

REACTION

REACTION DIAGRAM

LITERATURE

ENZYME 3D STRUCTURE

4.2.3.103

ent-copalyl diphosphate = ent-isokaur-15-ene + diphosphate

-

-

4.2.3.103

ent-copalyl diphosphate = ent-isokaurene + diphosphate

-

-

4.2.3.19

ent-copalyl diphosphate = ent-isokaur-15-ene + diphosphate

-

-

4.2.3.19

ent-copalyl diphosphate = ent-kaur-15-ene + diphosphate

-

-

4.2.3.103

2 entries

4.2.3.19

2 entries

Enzyme Kinetic Parameters

k_cat Value (Turnover Number) (2 results)

top print hide

Show Turnover Number (2 entries)

EC NUMBER

TURNOVER NUMBER [1/S]

TURNOVER NUMBER MAXIMUM [1/S]

COMMENTARY

LITERATURE

1.14.14.123

0.015

-

pH and temperature not specified in the publication

717875

1.14.14.76

0.01

-

pH and temperature not specified in the publication

717607

K_M Value (2 results)

top print hide

Show KM Value (2 entries)

EC NUMBER

KM VALUE [MM]

KM VALUE MAXIMUM [MM]

COMMENTARY

LITERATURE

1.14.14.123

0.015

-

pH and temperature not specified in the publication

717875

1.14.14.76

0.012

-

pH and temperature not specified in the publication

717607

References & Links

Literature References (5)

top print hide

Show Reference (5 entries)

BRENDA REFERENCE ID

PUBMED ID

TITLE

YEAR

AUTHORS

JOURNAL

VOLUME

PAGES

717607

21985968

Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7

2011

Wu, Y.; Hillwig, M.L.; Wang, Q.; Peters, R.J.

FEBS Lett.

585

3446-3451

717875

22215681

Characterization of CYP76M5-8 indicates metabolic plasticity within a plant biosynthetic gene cluster

2012

Wang, Q.; Hillwig, M.; Okada, K.; Yamazaki, K.; Wu, Y.; Swaminathan, S.; Yamane, H.; Peters, R.

J. Biol. Chem.

287

6159-6168

744147

25758958

Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis

2015

Kitaoka, N.; Wu, Y.; Xu, M.; Peters, R.J.

Appl. Microbiol. Biotechnol.

99

7549-7558

746144

29241565

Gene mapping and transcriptome profiling of a practical photo-thermo-sensitive rice male sterile line with seedling-specific green-revertible albino leaf

2018

Li, X.; He, Y.; Yang, J.; Jia, Y.; Zeng, H.

Plant Sci.

266

37-45

746311

26884192

Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis

2016

Mafu, S.; Jia, M.; Zi, J.; Morrone, D.; Wu, Y.; Xu, M.; Hillwig, M.L.; Peters, R.J.

Proc. Natl. Acad. Sci. USA

113

2526-2531

Links to other databases for ent-isokaurene

top print hide

Show Links (1 entry)

ChEBI

PubChem

-